Fumonisin B<sub>1</sub> production by <i>fusarium proliferatum</i> strain M5991 in a modified myro liquid medium

Dantzer, William R.; Pometto, AL; Murphy, Patricia A.

doi:10.1002/19960404nt4

Cited by 21 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The column was washed with 20% acetonitrile:80% water containing 0.1% trifluoroacetic acid (TFA), and FB 1 was eluted with 50% acetonitrile:50% water containing 0.1% TFA. The purity of the isolated FB1 was determined by analytical HPLC of the o-phthaldialdehyde (OPA) derivative (Hopmans and Murphy, 1993 Dantzer et al (1996a) by inoculating capped baffled Erlenmeyer flasks containing 500 mL of modified Myro medium with a 4 day shake flask culture of F. proliferatum strain M5991. Fumonisin B 1 was isolated and purified by the procedure as described in Dantzer et al (1996b).…”

Section: Methodsmentioning

confidence: 99%

Reaction with Fructose Detoxifies Fumonisin B₁ while Stimulating Liver-Associated Natural Killer Cell Activity in Rats

Dantzer

Hopmans

et al. 1997

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Fumonisin B1 (FB1) was reacted with fructose in an attempt to detoxify this mycotoxin. Fischer 344/N rats were initiated with diethylnitrosamine (15 mg/kg body weight) and then fed 69.3 μmol FB1/kg diet or 69.3 μmol FB1 reacted with fructose (FB1−fructose)/kg diet for 4 weeks. In comparison with the rats fed basal diet or FB1−fructose, the FB1-fed rats had significantly increased plasma cholesterol (P < 0.01), plasma alanine aminotransferase activity (P < 0.05), and endogenous hepatic prostaglandin production (P < 0.05). Placental glutathione S-transferase-positive and γ-glutamyl transferase-positive altered hepatic foci occurred only in the FB1-fed rats. Liver-associated natural killer (NK) cell activity was significantly decreased in the FB1-fed rats and increased in the group fed FB1-fructose, as compared with the basal group (P < 0.03). Therefore, modifying FB1 with fructose seems to prevent FB1-induced hepatotoxicity and promotion of hepatocarcinogenesis while stimulating liver-associated NK cell activity in rats. Fumonisin B 1 (FB 1 ) was reacted with fructose in an attempt to detoxify this mycotoxin. Fischer 344/N rats were initiated with diethylnitrosamine (15 mg/kg body weight) and then fed 69.3 µmol FB 1 /kg diet or 69.3 µmol FB 1 reacted with fructose (FB 1 -fructose)/kg diet for 4 weeks. In comparison with the rats fed basal diet or FB 1 -fructose, the FB 1 -fed rats had significantly increased plasma cholesterol (P < 0.01), plasma alanine aminotransferase activity (P < 0.05), and endogenous hepatic prostaglandin production (P < 0.05). Placental glutathione S-transferase-positive and γ-glutamyl transferase-positive altered hepatic foci occurred only in the FB 1 -fed rats. Liver-associated natural killer (NK) cell activity was significantly decreased in the FB 1 -fed rats and increased in the group fed FB 1 -fructose, as compared with the basal group (P < 0.03). Therefore, modifying FB 1 with fructose seems to prevent FB 1 -induced hepatotoxicity and promotion of hepatocarcinogenesis while stimulating liver-associated NK cell activity in rats.

show abstract

Section: Methodsmentioning

confidence: 99%

Reaction with Fructose Detoxifies Fumonisin B₁ while Stimulating Liver-Associated Natural Killer Cell Activity in Rats

Dantzer

Hopmans

et al. 1997

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…F. verticillioides MRC 826 corn culture material (after inoculation with 10À20 beads of fumonisin spores, in modified Myro medium (0.5 g/L of MgSO 4 ) plus 1% corn-hull-extract incubated for 69 days) containing 9807 μg of FB1/g was obtained from the Veterinary Medical Diagnostic Laboratory at the University of Missouri, Columbia, MO. 9 Rice culture infected with F. graminearum R6575 (containing 640 μg of deoxynivalenol/g and 57 μg of 15-acetyldeoxynivalenol/g), after inoculation on potato dextrose agar plates and incubation in carboxymethylcellulose media (composed of carboxymethylcellulose, NH 4 NO 3 3 7H 2 O, and yeast extract) mixed with enriched white rice, was prepared previously by Dr. Hendrich's laboratory according to the method of Clifford et al 10 Diet. Rats were divided into five groups: control (fed AIN93G diet obtained from Harlan Teklad, Madison, WI), Grainsure E (0.5% in AIN 93G), toxins (mixture of fumonisin B1, deoxynivalenol, and zearalenone in AIN93G), toxins + Grainsure E, and pair-fed controls (fed daily the mean food intake of toxin group as measured on the previous day).…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Efficacy of a Mycotoxin Binder against Dietary Fumonisin, Deoxynivalenol, and Zearalenone in Rats

Qiang

Truong²,

Meynen³

et al. 2011

J. Agric. Food Chem.

View full text Add to dashboard Cite

It was hypothesized that a mycotoxin binder, Grainsure E, would inhibit adverse effects of a mixture of fumonisin B1, deoxynivalenol, and zearalenone in rats. For 14 and 28 days, 8-10 Sprague-Dawley rats were fed control diet, Grainsure E (0.5%), toxins (7 μg fumonisin B1/g, 8 μg of deoxynivalenol/g and 0.2 μg of zearalenone/g), toxins (12 μg of fumonisin B1/g, 9 μg of deoxynivalenol/g, and 0.2 μg of zearalenone/g + Grainsure E), or pair-fed to control for food intake of toxin-fed rats. After 28 days, decreased body weight gain was prevented by Grainsure E in toxin-fed female rats, indicating partial protection against deoxynivalenol and fumonisin B1. Two effects of fumonisin B1 were partly prevented by Grainsure E in toxin-fed rats, increased plasma alanine transaminase (ALT) and urinary sphinganine/sphingosine, but sphinganine/sphingosine increase was not prevented in females at the latter time point. Grainsure E prevented some effects of fumonisin B1 and deoxynivalenol in rats. KeywordsAmes Laboratory, fumonisin; deoxynivalenol; zearalanone; mycotoxin binder; Grainsure E ABSTRACT: It was hypothesized that a mycotoxin binder, Grainsure E, would inhibit adverse effects of a mixture of fumonisin B1, deoxynivalenol, and zearalenone in rats. For 14 and 28 days, 8À10 SpragueÀDawley rats were fed control diet, Grainsure E (0.5%), toxins (7 μg fumonisin B1/g, 8 μg of deoxynivalenol/g and 0.2 μg of zearalenone/g), toxins (12 μg of fumonisin B1/g, 9 μg of deoxynivalenol/g, and 0.2 μg of zearalenone/g + Grainsure E), or pair-fed to control for food intake of toxin-fed rats. After 28 days, decreased body weight gain was prevented by Grainsure E in toxin-fed female rats, indicating partial protection against deoxynivalenol and fumonisin B1. Two effects of fumonisin B1 were partly prevented by Grainsure E in toxin-fed rats, increased plasma alanine transaminase (ALT) and urinary sphinganine/sphingosine, but sphinganine/sphingosine increase was not prevented in females at the latter time point. Grainsure E prevented some effects of fumonisin B1 and deoxynivalenol in rats.

show abstract

“…Fumonisin production of cultures grown in a modified Myro medium containing 1% (w/v) corn-hullextract [24] for 5 days on rotary shaker (125 rpm) at 25 °C, in the dark was measured by means of the Toxiklon ELISA kit [25].…”

Section: Secondary Metabolite Productionmentioning

confidence: 99%

Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum

Kohut

Oláh

Ádám

et al. 2010

J. Basic Microbiol.

View full text Add to dashboard Cite

A homologue of the adenylyl cyclase (AC) gene of Neurospora crassa, named Fpacy1 was cloned from the genomic library of Fusarium proliferatum ITEM 2287 by screening the library with a DNA fragment amplified by using PCR primers designed from conserved sequences of the catalytic domain of AC genes from other fungi. The deduced FPACY1 protein had 53-77% identity with the AC proteins of other fungi. DeltaFpacy1 mutants obtained by targeted gene disruption showed retarded vegetative growth, increased conidiation and delayed conidial germination. Colonization capability of the mutants, assessed on maize seedlings and tomato fruits also was adversely affected. In sexual crosses the AC mutants retained full male fertility, but their female fertility decreased significantly. Disruption of Fpacy1 abolished vegetative self-incompatibility, suggesting that the AC gene is involved in multiple developmental processes related to vegetative growth, as well as sexual and parasexual events. The elevated thermo- and H(2)O(2)-tolerance of the DeltaFpacy1 mutants was coupled to an increased sensitivity towards Cd and Cu, indicating that the cAMP signaling pathway may have both negative and positive regulatory roles on the stress response mechanisms of fungal cells. When grown under nitrogen limitation conditions, the DeltaFpacy1 mutants produced an average of approximately 274 microg g(-1) bikaverin, whereas only traces of this metabolite was detected in the wild type. This finding provides further evidence of the role of the cAMP-PKA pathway in regulating bikaverin production.

show abstract

Fumonisin B₁ production by fusarium proliferatum strain M5991 in a modified myro liquid medium

Cited by 21 publications

References 17 publications

Reaction with Fructose Detoxifies Fumonisin B₁ while Stimulating Liver-Associated Natural Killer Cell Activity in Rats

Reaction with Fructose Detoxifies Fumonisin B₁ while Stimulating Liver-Associated Natural Killer Cell Activity in Rats

Efficacy of a Mycotoxin Binder against Dietary Fumonisin, Deoxynivalenol, and Zearalenone in Rats

Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum

Contact Info

Product

Resources

About

Fumonisin B1 production by fusarium proliferatum strain M5991 in a modified myro liquid medium

Cited by 21 publications

References 17 publications

Reaction with Fructose Detoxifies Fumonisin B1 while Stimulating Liver-Associated Natural Killer Cell Activity in Rats

Reaction with Fructose Detoxifies Fumonisin B1 while Stimulating Liver-Associated Natural Killer Cell Activity in Rats

Efficacy of a Mycotoxin Binder against Dietary Fumonisin, Deoxynivalenol, and Zearalenone in Rats

Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum

Contact Info

Product

Resources

About

Fumonisin B₁ production by fusarium proliferatum strain M5991 in a modified myro liquid medium

Reaction with Fructose Detoxifies Fumonisin B₁ while Stimulating Liver-Associated Natural Killer Cell Activity in Rats

Reaction with Fructose Detoxifies Fumonisin B₁ while Stimulating Liver-Associated Natural Killer Cell Activity in Rats