Fully room temperature and label free biosensing based on an ink-jet printed polymer microdisk laser

Abstract

Materials with biomolecule-compatible functional groups are desirable for the fabrication of microdisk lasers used in bio-sensing applications. In this study, a microdisk laser was fabricated using a low-viscosity hyper branched polymer FC-V-50 using ink-jet printing, and was surface-modified at room temperature within a relatively short time compared to conventional methods. The carboxyl functional group of the FC-V-50 polymer was used for surface modification and biotinylation. The adsorption characteristics of the microdisk laser were evaluated using bovine serum albumin, avidin, and streptavidin. This study reports the first demonstration of label-free biosensing using the FC-V-50 polymer-based microdisk laser.

H3 (Hydrogel‐Based, High‐Sensitivity, Hybrid) Plasmonic Transducers for Biomolecular Interactions Monitoring

“…Chemicals and Reagents: Trisodium citrate (Na 3 C 6 H 5 O 7 ), tetrachloroauric acid (HAuCl 4 ), Poly(Ethylene Glycol) Diacrylate (PEGDA, average Mn 10 000), 2-Hydroxy-2-methylpropiophenone (Darocur 1173), cysteamine, sulfo-N-hydroxysuccinimide biotin (biotin-NHS) watersoluble, Bovine Serum Albumin (BSA), Phosphate Buffered Saline (PBS), Tween20, Streptavidin (SA) from Streptomyces avidinii, and Streptavidin− Cy3 (Cy3-SA) from Streptomyces avidinii, Protein G from Streptococcus sp. Ink-jet printed polymer microdisk laser 1.9 1.9-94 [57] Poly T-templated copper NPs 0.1 0.5-1000 [58] LSPR biosensor based on Au nanorods 2.0 2.0-50 [44] Present work Nonlabeled SA Cy3-SA 1.6 0.0001 1.5-750 0.0001-150 were purchased from Merck KGaA (DE); Wheat Germ Agglutinin (WGA), Alexa Fluor 555 Conjugate was purchased from Thermo Fisher Scientific.…”

“…The results obtained with the proposed transducer were very promising and competitive with other platforms, which have been recently reported. [44,45,[56][57][58] The direct comparison between the results achieved with the H 3 transducer as a novel functional material and the platforms available in the literature for SA sensing is summarized in Table 1. The high MW PEGDA hydrogel combined with AuNPs enabled the design of a simple and efficient platform with a good LOD in labelfree mode and a broad dynamic range, which could be mainly ascribed to the 3D nature of our platform.…”

Device parameter to evaluate exciton energy transfer in organic whispering-gallery-mode microresonators and its dependence on the amplified spontaneous emission threshold

“…Because of their high quality factors and small mode volumes, WGMs have attracted much attention for potential applications in optical devices, such as lasers [1][2][3] and sensors. [4][5][6][7][8] WGM resonators are constructed from spheres, 1 disks, 9 and other microstructures, [10][11][12] and thus the standing modes are controllable by combining their unit structures (i.e., a microresonator) with inter-resonator structures. Owing to the similarity to the wavefunctions of electrons, the former and latter are called photonic atoms 13 and photonic molecules, 14 respectively.…”

Estimation of the dynamic range of the angular velocity sensor based on a fiber-optic resonator

“…Такие резонаторы находят применение в самых различных областях науки и техники: в лазерной технике для стабилизации частоты излучения [1], в метрологии для сверхточного измерения перемещений [2], в спектроскопии для измерения концентрации различных веществ [3], в биофизике для определения наличия патогенов [4], в качестве неклассических источников фотонов [5], датчиков температуры [6], оптоэлектронных генераторов [7] и т.д.…”