Fully Dried Two-Dimensional Paper Network for Enzymatically Enhanced Detection of Nucleic Acid Amplicons

Byers, Kristin M.; Bird, Anna R.; Cho, HyunDae D.; Linnes, Jacqueline C.

doi:10.1021/acsomega.0c00115

Cited by 13 publications

(14 citation statements)

References 25 publications

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“…Other paper-based devices have been developed to diagnose bacterial infections [24][25][26][27][28] , detect viral infections 29 , and concentrate infectious DNA 30 , but only a few paper platforms perform some kind of susceptibility testing. Deiss et al 31 portable paper chip replicates the Kirby-Bauer disk diffusion method using PrestoBlue, but the readout requires measuring the zone of inhibition around each disk and comparing the results to published CLSI standards.…”

Section: Discussionmentioning

confidence: 99%

Multiplexed paper-based assay for personalized antimicrobial susceptibility profiling of Carbapenem-resistant Enterobacterales performed in a rechargeable coffee mug

Oeschger

Kret

Erickson

2022

Sci Rep

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The increasing prevalence of antibiotic resistance threatens to make currently treatable bacterial diseases deadly again. As drug resistance rises, antibiotic susceptibility testing needs to adapt to allow for widespread, individualized testing. Paper-based diagnostics offer low-cost, disposable alternatives to traditional time consuming and costly in-house methods. Here, we describe a paper-based microfluidic device, called the Bac-PAC, capable of categorizing the antibiotic susceptibly of individual strains of Carbapenem-resistant Enterobacterales. Each chip provides a colored readout with actionable susceptibility classification of three antibiotics, thus maximizing the chances of identifying a viable therapy. We verified the technology on thirty bacterial strains with two dyes using six clinically relevant antibiotics. We demonstrated that the dried tests are stable for one month and can be incubated in a rechargeable coffee mug that reduces the need for external infrastructure.

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Section: Discussionmentioning

confidence: 99%

Multiplexed paper-based assay for personalized antimicrobial susceptibility profiling of Carbapenem-resistant Enterobacterales performed in a rechargeable coffee mug

Oeschger

Kret

Erickson

2022

Sci Rep

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“… 68 Similarly, Byers et al developed a 2D paper network to perform immunoassay for the detection of nucleic acids of SARS-CoV-2 with the POCT format. 69 …”

Section: Emerging Techniques To Detect Sars-cov-2mentioning

confidence: 99%

“…68 Similarly, Byers et al developed a 2D paper network to perform immunoassay for the detection of nucleic acids of SARS-CoV-2 with the POCT format. 69 Although nucleic acid based lateral flow assays are sensitive, lateral flow immunoassays have gained interest in the massive surveillance of the COVID-19 pandemic because of their simplicity and cheap cost. Currently, IgM/IgG rapid test kits are available for qualitative antibody testing of COVID-19.…”

Section: Diagnostic Testing Has Become Indispensable For Diagnosis Pr...mentioning

confidence: 99%

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Detecting the Coronavirus (COVID-19)

2020

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The COVID-19 pandemic has created huge damage to society and brought panic around the world. Such panic can be ascribed to the seemingly deceptive features of COVID-19: Compared to other deadly viral outbreaks, it has medium transmission and mortality rates. As a result, the severity of the causative coronavirus, SARS-CoV-2, was deeply underestimated by society at the beginning of the COVID-19 outbreak. Based on this, in this review, we define the viruses with features similar to those of SARS-CoV-2 as the Panic Zone viruses. To contain those viruses, accurate and fast diagnosis followed by effective isolation and treatment of patients are pivotal at the early stage of virus breakouts. This is especially true when there is no cure or vaccine available for a transmissible disease, which is the case for the current COVID-19 pandemic. As of July 2020, more than 100 kits for COVID-19 diagnosis on the market have been surveyed in this review, while emerging sensing techniques for SARS-CoV-2 are also discussed. It is of critical importance to rationally use these kits for efficient management and control of the Panic Zone viruses. Therefore, we discuss guidelines to select diagnostic kits at different outbreak stages of the Panic Zone viruses, SARS-CoV-2 in particular. While it is of utmost importance to use nucleic acid based detection kits with low false negativity (high sensitivity) at the early stage of an outbreak, the low false positivity (high specificity) gains importance at later stages of the outbreak. When society is set to reopen from the lockdown stage of the COVID-19 pandemic, it becomes critical to have immunoassay based kits with high specificity to identify people who can safely return to society after their recovery from SARS-CoV-2 infections. Finally, since a massive attack from a viral pandemic requires a massive defense from the whole society, we urge both government and the private sector to research and develop affordable and reliable point-of-care testing (POCT) kits, which can be used massively by the general public (and therefore called massive POCT) to contain Panic Zone viruses in the future.

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“…Applying CNT‐functionalized fibronectin based on protein for a conductance biosensor construction to detect the SARS virus is one of the applications of these nanomaterials. 331 Also, antibody‐coated graphene sheet has led to the development of a voltammetric biosensor for SARS‐Covid 2 detection. 324 …”

Section: Nanotechnology and Corona Virusesmentioning

confidence: 99%

COVID‐19: A systematic review and update on prevention, diagnosis, and treatment

Aliabadi

Eivazzadeh‐Keihan

Parikhani

et al. 2022

MedComm

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Since the rapid onset of the COVID‐19 or SARS‐CoV‐2 pandemic in the world in 2019, extensive studies have been conducted to unveil the behavior and emission pattern of the virus in order to determine the best ways to diagnosis of virus and thereof formulate effective drugs or vaccines to combat the disease. The emergence of novel diagnostic and therapeutic techniques considering the multiplicity of reports from one side and contradictions in assessments from the other side necessitates instantaneous updates on the progress of clinical investigations. There is also growing public anxiety from time to time mutation of COVID‐19, as reflected in considerable mortality and transmission, respectively, from delta and Omicron variants. We comprehensively review and summarize different aspects of prevention, diagnosis, and treatment of COVID‐19. First, biological characteristics of COVID‐19 were explained from diagnosis standpoint. Thereafter, the preclinical animal models of COVID‐19 were discussed to frame the symptoms and clinical effects of COVID‐19 from patient to patient with treatment strategies and in‐silico/computational biology. Finally, the opportunities and challenges of nanoscience/nanotechnology in identification, diagnosis, and treatment of COVID‐19 were discussed. This review covers almost all SARS‐CoV‐2‐related topics extensively to deepen the understanding of the latest achievements (last updated on January 11, 2022).

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Fully Dried Two-Dimensional Paper Network for Enzymatically Enhanced Detection of Nucleic Acid Amplicons

Cited by 13 publications

References 25 publications

Multiplexed paper-based assay for personalized antimicrobial susceptibility profiling of Carbapenem-resistant Enterobacterales performed in a rechargeable coffee mug

Multiplexed paper-based assay for personalized antimicrobial susceptibility profiling of Carbapenem-resistant Enterobacterales performed in a rechargeable coffee mug

Detecting the Coronavirus (COVID-19)

COVID‐19: A systematic review and update on prevention, diagnosis, and treatment

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