Fully automated laser ablation liquid capture surface analysis using nanoelectrospray ionization mass spectrometry

Lorenz, Matthias; Ovchinnikova, Olga S.; Berkel, Gary J. Van

doi:10.1002/rcm.6904

Cited by 21 publications

(25 citation statements)

References 34 publications

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“…This assumes 100% extraction efficiency in the case of the direct liquid extraction, and this is a reasonable assumption because the spot sampled (ca 50 µm square) was much smaller than the size of the liquid junction (at least 1.9 mm) and because the blue ink was very soluble in the methanol extraction solvent. This estimated capture efficiency was more than double the efficiency that we have reported with LA into a horizontally configured non‐contact continuous flow LMJ‐SSP/ESI emitter probe or with LA into a suspended droplet …”

Section: Resultsmentioning

confidence: 61%

Transmission geometry laser ablation into a non‐contact liquid vortex capture probe for mass spectrometry imaging

Ovchinnikova

Bhandari

Lorenz

et al. 2014

Rapid Comm Mass Spectrometry

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Section: Resultsmentioning

confidence: 61%

Transmission geometry laser ablation into a non‐contact liquid vortex capture probe for mass spectrometry imaging

Ovchinnikova

Bhandari

Lorenz

et al. 2014

Rapid Comm Mass Spectrometry

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“…MSI can map the localization of ions across a selected surface. [Zebrafish ion images in (B) reproduced from Lostun et al 50 ] Single Probe (SP) 16 Liquid extraction 10-20 μm (8.5 μm) a Liquid Microjunction-Surface Sampling Probe (LMJ-SSP) 17 Liquid extraction 0.5-1 mm (0.5 mm) a Laser Ablation-Liquid Capture Surface Analysis (LA-LCSA) 18 Liquid extraction (50 μm) a…”

Section: Ambient Ms Methods With Imaging Capabilitiesmentioning

confidence: 99%

Review and perspectives on the applications of mass spectrometry imaging under ambient conditions

Pérez

Bagga

Prova

et al. 2018

Rapid Comm Mass Spectrometry

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Ambient mass spectrometry (AMS)-based techniques are performed under ambient conditions in which the ionization and desorption occur in the open environment allowing the direct analysis of molecules with minimal or no sample preparation. A selected group of AMS techniques demonstrate imaging capabilities that can provide information about the localization of molecules on complex sample surfaces such as biological tissues. 2D, 3D, and multimodal imaging have unlocked an array of applications to systematically address complex problems in many areas of research such as drug monitoring, natural products, forensics, and cancer diagnostics. In the present review, we summarize recent advances in the field with respect to the implementation of new ambient ionization techniques and current applications in the last 5 years. In more detail, we mainly focus on imaging applications in topics related to animal whole bodies and tissues, single cells, cancer diagnostics and biomarkers, microbial cultures and co-cultures, plant and natural product metabolomics, and forensic applications. Finally, we discuss new areas of research, future perspectives, and the overall direction that the field may take in the years to come.

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“…The first category [22,23] is represented by the out-of-plane devices, where 100 or 400 nanospray emitters are integrated onto a single silicon substrate, from which electrospray is established perpendicular to the substrate. These systems are known under the commercial name of NanoMate robot (Fig.…”

Section: Nanochips For Electrospray Ionization Mass Spectrometrymentioning

confidence: 99%

Nanofluidics-Based Mass Spectrometry. Applications for Biomarker Discovery in Lysosomal Storage Diseases

Sârbu

Zamfir

2014

Nanoparticles' Promises and Risks

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Substantial efforts are currently invested in development of micro-and nanofluidics-based systems as front end technology for electrospray ionization (ESI) mass spectrometry (MS). Since its first introduction in biological MS chipbased ESI demonstrated a high potential to discover novel biopolymer species due to the efficient ionization properties, preferential formation of multiply charged ions and the elevated reproducibility and sensitivity. In combination with highresolution mass spectrometers or instruments able to perform multistage fragmentation, chip-electrospray confirmed its unique ability to offer structural elucidation of minor species in complex mixtures, which often represent valuable biomarkers of severe diseases. This aspect is of particular importance for the applicability of chip MS in clinical investigation where only minute amounts of biological material are available. In view of these major advantages of nanofluidics in conjunction with modern MS, this chapter reviews the strategies, which allowed a successful application of chip technology for early diagnostic of lysosomal storage diseases. The first part is dedicated to the principles of ESI MS and to advanced nanochip systems for ESI MS. The second part highlights important achievements of nanochip ESI MS in biomarker discovery and diagnostic of LSDs such as Fabry and Schindler diseases. Finally, this chapter emphasizes that advanced chip ESI MS has real perspectives to become a routine method for early diagnosis and therapy of severe pathologies such as LSDs.

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Fully automated laser ablation liquid capture surface analysis using nanoelectrospray ionization mass spectrometry

Cited by 21 publications

References 34 publications

Transmission geometry laser ablation into a non‐contact liquid vortex capture probe for mass spectrometry imaging

Transmission geometry laser ablation into a non‐contact liquid vortex capture probe for mass spectrometry imaging

Review and perspectives on the applications of mass spectrometry imaging under ambient conditions

Nanofluidics-Based Mass Spectrometry. Applications for Biomarker Discovery in Lysosomal Storage Diseases

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