Fully Automated Determination of Cannabinoids in Hair Samples using Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry

Mußhoff, Frank; Junker, Heike; Lachenmeier, Dirk W.; Kroener, Lars; Madea, Burkhard

doi:10.1093/jat/26.8.554

Cited by 75 publications

(59 citation statements)

References 24 publications

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“…The mixture was diluted with deionized water (1:100). Then, the mixture was vortexed for 1 minute [5]. Finally, the supernatant is the effective sample that can be aspirated and applied to the biochip immuno-analyzer array technology analyzer (Randox/Drugs of Abuse BAT Array).…”

Section: Toxicological Investigationsmentioning

confidence: 99%

Validity of Substances of Abuse Detection in Hair by Biochips Array Technology

Ragab¹,

Al-Mazroua²,

El-Harouny³

et al. 2018

J Alcohol Drug Depend

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Using hair as a biological specimen to analyze drug abuse has been receiving elevated attention during last years because of flexibility of collection procedure and because hair does not autolysis like other body fluids or tissues. Hair testing provides a broad-spectrum detection window after drug exposure than urine testing. Hair analysis has specific criteria that it provides a way of obtaining information that cannot be acquired by other commonly used forensic toxicology analytical procedures, such as blood or urine analysis. In the hair matrix, many xenobiotics are trapped permanently, in disparity to the situation with blood or urine where they are only identifiable for a few hours or days. Subsequently substance of abuse detection by hair analysis should be the target procedure in the clinical and forensic toxicology aspect when the evaluation of repeated or chronic exposure to a drug is indicated, e.g. in the case of pre-employment/workplace testing, criminal affairs or a driving license restoration. In the current study, seventy cases of the application of hair analyses using this technique for the determination of substances of abuse (opiates, cannabis, amphetamine, barbiturate, benzodiazepines, and cocaine) are investigated. Analytical toxicology procedure for detection of substance of abuse is critical important: Biochips array technology that confirmed with GC/MS has proved to be a highly sensitive and specific technique for the detection of substances of abuse at very low concentrations in hair except for cannabis.

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Section: Toxicological Investigationsmentioning

confidence: 99%

Validity of Substances of Abuse Detection in Hair by Biochips Array Technology

Ragab¹,

Al-Mazroua²,

El-Harouny³

et al. 2018

J Alcohol Drug Depend

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“…7.4). Reagents used for this purpose have high volatility, such as silylation reagents including n-methyl-n-(trimethylsilyl)trifluoroacetamide (MSTFA) [38], nmethyl-n-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) [39], hexamethyldisilazane [40], n-methylbis(trifluoroacetamide) [41], pentafluorobenzoyl chloride [42], or trifluoroacetic anhydride [43]. After DI SPME with appropriate fiber, on-fiber post-derivatization with silylation reagents have mainly focused on the analysis of aliphatic amines, phenolic compounds, acid pesticides, …”

Section: On-fiber Post-derivatizationmentioning

confidence: 99%

Application of Solid-Phase Microextraction Combined with Derivatization for Polar Compound Sampling in Environmental Analysis

Yang¹,

Luan²

2016

Solid Phase Microextraction

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Solid-phase microextraction (SPME) is a solvent-free sample preparation technique using a fused-silica fiber coated with an appropriate stationary phase. Derivatization is often necessary to determine substances with poor chromatographic behavior, high reactivity, and/or volatility or thermal instability. SPME derivatization technique can be operated under different combined modes and may be influenced by factors such as sample matrix, SPME, derivatization, and desorption condition. Combined with derivatization, HS-SPME, DI-SPME, membrane-protected SPME, and in-tube SPME have been applied to the analysis of a vast amount of chemicals, i.e., aldehydes and acetone, amines, organic acids, phenolic compounds, pesticide, metal and organic metal species, and pharmaceutical and personal care products (PPCPs) in a wide range of environmental matrices, including air, water, soil, and sediment samples. This chapter reviews the mode and method development of the derivatization technique, the derivatization reagents, and reaction mechanism, as well as its applications in environmental analysis.

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“…Automated in-fibre (or more accurately in-coating) derivatisation methods have been reported, for example in the analysis of aldehydes with pentafluorobenzyl hydroxylamine (PFBHA) derivatisation [40,41], cannabinoids with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) derivatisation [42,43] and carboxylic acids with pyrenyldiazomethane (PDAM) [44,45] derivatisation. The in-tube SPDE approach has also been used for in-coating derivatisation applications [30,32,34].…”

Section: Automation Of Procedures Involving In-fibre Derivatisationmentioning

confidence: 99%

Automation of solid‐phase microextraction

O’Reilly

Wang

Šetková

et al. 2005

J of Separation Science

107

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This review discusses developments and future challenges in the automation of solidphase microextraction (SPME). The emphasis of the review is placed on automated SPME-GC using fibre and in-tube configurations, and includes discussion of recent developments that may have significant implications for automation such as superelastic fibre assemblies and internally cooled fibre-SPME. Existing methods used for automated SPME-LC are summarised together with a more detailed overview of recent developments such as using solvent desorption followed by syringe injection using a robotic system. Progress towards automation of other SPME configurations is also discussed.

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Fully Automated Determination of Cannabinoids in Hair Samples using Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry

Cited by 75 publications

References 24 publications

Validity of Substances of Abuse Detection in Hair by Biochips Array Technology

Validity of Substances of Abuse Detection in Hair by Biochips Array Technology

Application of Solid-Phase Microextraction Combined with Derivatization for Polar Compound Sampling in Environmental Analysis

Automation of solid‐phase microextraction

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