Full sensitivity of P<sub>2times2</sub> purinoceptor to ATP revealed by changing extracellular pH

King, Brian F.; Ziganshina, L. E.; Pintor, Jesús; Burnstock, Geoffrey

doi:10.1111/j.1476-5381.1996.tb15293.x

Cited by 129 publications

(125 citation statements)

References 11 publications

(19 reference statements)

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“…This inhibition can be relieved by increasing extracellular calcium. These results are in contrast to studies on P2X 2 receptors in which responses to ATP may be potentiated by acidification (King et al 1996). The P2X 4 receptor ion channel is thus likely to be functionally modulated by the level of surrounding synaptic activity via local changes in extracellular pH and calcium in a way that differs from other P2X receptors.…”

Section: * Laboratory For Molecular Cell Biology and T Department Of contrasting

confidence: 54%

“…1996) whilst P2X 2 receptor-mediated responses have been shown to be potentiated by acidification (King et al 1996). In the present study we have investigated the effects of altering extracellular pH on a brain-localized P2X receptor (Garcia-Guzman et al 1997), the human P2X 4 receptor, expressed in Xenopusoocytes.…”

Section: * Laboratory For Molecular Cell Biology and T Department Of mentioning

confidence: 99%

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General – Ion Channels

1998

The Journal of Physiology

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Section: * Laboratory For Molecular Cell Biology and T Department Of contrasting

confidence: 54%

Section: * Laboratory For Molecular Cell Biology and T Department Of mentioning

confidence: 99%

General – Ion Channels

1998

The Journal of Physiology

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“…P2X 2 homomeric receptors show only an increase in peak currents when pH is changed from control (pH 7.4) to a pH of 6.5 [26,45,51]. In addition, pH variations to 5.5 caused an increase in P2X 2 peak currents but a decrease on P2X 2/6 peak currents [27].…”

Section: Discussionmentioning

confidence: 95%

Mouse Leydig cells express multiple P2X receptor subunits

Antonio

Costa

Gomes

et al. 2008

Purinergic Signalling

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ATP acts on cellular membranes by interacting with P2X (ionotropic) and P2Y (metabotropic) receptors. Seven homomeric P2X receptors (P2X 1 -P2X 7 ) and seven heteromeric receptors (P2X 1/2 , P2X 1/4 , P2X 1/5 , P2X 2/3 , P2X 2/6 , P2X 4/6 , P2X 4/7 ) have been described. ATP treatment of Leydig cells leads to an increase in [Ca 2+ ] i and testosterone secretion, supporting the hypothesis that Ca 2+ signaling through purinergic receptors contributes to the process of testosterone secretion in these cells. Mouse Leydig cells have P2X receptors with a pharmacological and biophysical profile resembling P2X 2 . In this work, we describe the presence of several P2X receptor subunits in mouse Leydig cells. Western blot experiments showed the presence of P2X 2 , P2X 4 , P2X 6 , and P2X 7 subunits. These results were confirmed by immunofluorescence. Functional results support the hypothesis that heteromeric receptors are present in these cells since 0.5 μM ivermectin induced an increase (131.2±5.9%) and 3 μM ivermectin a decrease (64.2±4.8%) in the whole-cell currents evoked by ATP. These results indicate the presence of functional P2X 4 subunits. P2X 7 receptors were also present, but they were non-functional under the present conditions because dye uptake experiments with Lucifer yellow and ethidium bromide were negative. We conclude that a heteromeric channel, possibly P2X 2/4/6 , is present in Leydig cells, but with an electrophysiological and pharmacological phenotype characteristic of the P2X 2 subunit.

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“…We have shown recently that ATP-evoked excitation of inspiratory neurones underlies carbon dioxide-mediated excitation of respiratory activity . Our observation that P2X 2 reactivity was present in the VLM is therefore particularly signi¢cant, since this receptor subtype is known to be highly sensitive to changes in pH (King et al, 1996).…”

Section: Nsc 5204 5-11-01mentioning

confidence: 90%

Evidence for the involvement of purinergic signalling in the control of respiration

et al. 2001

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AbstractöThe ventrolateral medulla has a critical role in the generation and patterning of respiration via an extensive network of respiratory neurones. We have investigated the e¡ects of activating purinergic P2 receptors within the ventrolateral medulla of the anaesthetised rat on the overall pattern of respiratory activity. In addition, using immunohistochemical techniques, we have identi¢ed the subtypes of P2X receptors in the ventrolateral medulla. Unilateral microinjection of ATP into the ventrolateral medulla reduced in a dose-dependent manner, or abolished, resting phrenic nerve discharge recorded as an indication of central inspiratory drive. ATP also elicited increases in blood pressure and variable changes in heart rate. These e¡ects were mimicked by microinjection of the P2X receptor agonist K,L-methylene ATP into the ventrolateral medulla. Whilst microinjection of suramin, a P2 receptor antagonist, had no e¡ect on resting cardiorespiratory variables it blocked the respiratory and cardiovascular e¡ects of ATP microinjected into the ventrolateral medulla. Immunohistochemical staining using IgG antibodies showed that P2X 1 , P2X 2 , P2X 5 and P2X 6 , but not P2X 3 , P2X 4 or receptor subunits were localised in the rostral ventrolateral medulla.Our results indicate that several P2X receptor subtypes are localised within areas of the ventrolateral medulla that are important for cardiorespiratory control (including the pre-Bo « tzinger and Bo «tzinger complexes), and that activation of these receptors can have profound e¡ects on both the cardiovascular and the respiratory networks. Our pharmacological data suggest that di¡erent P2X subunits in this region may co-assemble to form hetero-oligomeric assemblies as well as homomultimers within this region. ß

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Full sensitivity of P_2times2 purinoceptor to ATP revealed by changing extracellular pH

Cited by 129 publications

References 11 publications

General – Ion Channels

General – Ion Channels

Mouse Leydig cells express multiple P2X receptor subunits

Evidence for the involvement of purinergic signalling in the control of respiration

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Full sensitivity of P2times2 purinoceptor to ATP revealed by changing extracellular pH

Cited by 129 publications

References 11 publications

General – Ion Channels

General – Ion Channels

Mouse Leydig cells express multiple P2X receptor subunits

Evidence for the involvement of purinergic signalling in the control of respiration

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Full sensitivity of P_2times2 purinoceptor to ATP revealed by changing extracellular pH