Mouse Leydig cells express multiple P2X receptor subunits

Antonio, Ligia Subitoni; Costa, Roberta Ribeiro; Gomes, Marcelo D.; Varanda, Wamberto Antônio

doi:10.1007/s11302-008-9128-9

Cited by 26 publications

(30 citation statements)

References 52 publications

(87 reference statements)

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“…The presence of ecto-5 0 -nucleotidase in interstitial cells was detected in both macrophages, which also express NTPDase1, and Leydig cells. Although the P2 receptor pattern in mouse Leydig cells (Antonio et al 2009;Glass et al 2001) and extracellular ATP stimulation of testosterone secretion (Foresta et al 1996) have been welldefined, adenosine receptors have not yet been identified at interstitial cells and would be an interesting issue to address.…”

Section: Discussionmentioning

confidence: 99%

High expression and activity of ecto-5′-nucleotidase/CD73 in the male murine reproductive tract

Martı́n-Satué

Lavoie

Fausther

et al. 2010

Histochem Cell Biol

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Extracellular ATP and its hydrolysis product adenosine modulate various reproductive functions such as those requiring contraction, steroidogenesis, and maintenance of fluid composition. Interestingly, adenosine might act as a key capacitative effector for mammalian spermatozoa to acquire the capacity for fertilisation. Extracellular nucleotide levels are affected by cell surface ectonucleotidases, amongst which the ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) family regroups the most abundant and effective enzymes to hydrolyse ATP and ADP to AMP in physiological conditions. In the male reproductive tract three members of this family have been indentified: NTPDase1, NTPDase2 and NTPDase3 (Martín-Satué et al. in Histochem Cell Biol 131:615-628, 2009). The purpose of the present study was to characterize in the male reproductive tract the expression profile of the main enzyme responsible for the generation of adenosine from AMP, namely the ecto-5'-nucleotidase (CD73). The enzyme was identified by immunological techniques and by in situ enzymatic assays, including inhibition experiments with alpha,beta-methylene-ADP, a specific CD73 inhibitor. High levels of ecto-5'-nucleotidase were detected in testes in association with both germinal and somatic cells, in smooth muscle cells throughout the tract, in secretory epithelia from exocrine glands, and remarkably, in principal cells of epididymis, where co-localization with NTPDase3 was found. The relevance of this co-expression on nucleotide hydrolysis in these cells directly involved in the control of sperm fluid composition was addressed biochemically. This study suggests close regulation of extracellular nucleoside and nucleotide levels in the genital tract by ecto-5'-nucleotidase that, in concurrence with NTPDases, may impact male fertility.

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Section: Discussionmentioning

confidence: 99%

High expression and activity of ecto-5′-nucleotidase/CD73 in the male murine reproductive tract

Martı́n-Satué

Lavoie

Fausther

et al. 2010

Histochem Cell Biol

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“…IVM-sensitive currents were also detected in numerous mouse cell types, including macrophages (Sim et al, 2007), cortical neurons (Lalo et al, 2007), glial cells (Raouf et al, 2007), ventricular myocytes (Shen et al, 2006), submandibular gland ductal cells (Pochet et al, 2007), and Leydig cells (Antonio et al, 2009). IVM was also used to study the functional interactions of P2X4R with other members of this family of channels (Alqallaf et al, 2009;CasasPruneda et al, 2009).…”

Section: Ivermectin As the P2x4 Receptor-specific Modulatormentioning

confidence: 99%

Activation and Regulation of Purinergic P2X Receptor Channels

et al. 2011

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“…Thus, activation of p38 MAPK may be a critical mechanistic step of convergence in the P2X7 and P2X4 receptor signaling pathways in neuropathic pain. P2X7 and P2X4 receptors also interact to form complexes with unique channel properties (Antonio et al, 2009;Boumechache et al, 2009;Casas-Pruneda et al, 2009;Guo et al, 2007;Nicke, 2008). This raises the interesting possibility that structural and functional interactions, as well as converging signaling pathways, might be critical cellular mechanisms that underlie involvement of microglial P2X7 and P2X4 receptors in neuropathic pain.…”

Section: Role Of Microglial P2x7 Receptors In Neuropathic Painmentioning

confidence: 99%

ATP receptors gate microglia signaling in neuropathic pain

Trang

Beggs

Salter

2012

Experimental Neurology

136

104

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Microglia were described by Pio del Rio-Hortega (1932) as being the 'third element' distinct from neurons and astrocytes. Decades after this observation, the function and even the very existence of microglia as a distinct cell type was a topic of intense debate and conjecture. However, considerable advances have been made towards understanding the neurobiology of microglia resulting in a radical shift in our view of them as being passive bystanders that have solely immune and supportive roles, to being active principal players that contribute to central nervous system pathologies caused by disease or following injury. Converging lines of evidence implicate microglia as being essential in the pathogenesis of neuropathic pain, a debilitating chronic pain condition that can occur after peripheral nerve damage caused by disease, infection, or physical injury. A key molecule that modulates microglial activity is ATP, an endogenous ligand of the P2-purinoceptor family consisting of P2X ionotropic and P2Y metabotropic receptors. Microglia express several P2 receptor subtypes, and of these the P2X4, P2X7, and P2Y12 receptor subtypes have been implicated in neuropathic pain. The P2X4 receptor has emerged as the core microglianeuron signaling pathway: activation of this receptor causes release of brain-derived neurotrophic factor (BDNF) which causes disinhibition of pain-transmission neurons in spinal lamina I. The present review highlights recent advances in understanding the signaling and regulation of P2 receptors expressed in microglia and the implications for microglia-neuron interactions for the management of neuropathic pain. KeywordsMicroglia; P2 purinoceptors; Neuropathic pain; Nerve injury; ATP; BDNF; spinal cord Pain is a double-edged sword that can be protective or cause considerable suffering. Acute nociceptive pain warns against imminent or existing tissue damage, whereas chronic pain has no known defensive or beneficial function and is unremitting for those who suffer from this condition. Acute pain is produced by physiological functioning of the normal peripheral and central nervous systems. However, the processes initiated during acute pain can sometimes progress to chronic pain that is characterized as persisting long after the initiating event has healed. This transition to chronicity is highly variable between individuals and the degree of injury is not necessarily predictive of the severity or chronicity of the pain. There is mounting evidence that the transition from acute to chronic pain involves discrete CIHR Author ManuscriptCIHR Author Manuscript CIHR Author Manuscript pathophysiological steps that alter the cellular, molecular, and anatomical organization of nociceptive neural networks in the spinal dorsal horn (Latremoliere and Woolf, 2009;Scholz and Woolf, 2002;Voscopoulos and Lema, 2010;Woolf and Salter, 2000). In this pathologically altered system, the balance of inhibitory and excitatory control is shifted such that inhibitory mechanisms are weakened while excitatory mechanisms ar...

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Mouse Leydig cells express multiple P2X receptor subunits

Cited by 26 publications

References 52 publications

High expression and activity of ecto-5′-nucleotidase/CD73 in the male murine reproductive tract

High expression and activity of ecto-5′-nucleotidase/CD73 in the male murine reproductive tract

Activation and Regulation of Purinergic P2X Receptor Channels

ATP receptors gate microglia signaling in neuropathic pain

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