Full-Range Intracellular pH Sensing by an Aggregation-Induced Emission-Active Two-Channel Ratiometric Fluorogen

Chen, Sijie; Hong, Yuning; Liu, Yang; Liu, Jianzhao; Leung, Chi Man; Li, Min; Kwok, Ryan T. K.; Zhao, Engui; Lam, Jacky W. Y.; Yu, Yong; Tang, Ben Zhong

doi:10.1021/ja400337p

Cited by 401 publications

(255 citation statements)

References 23 publications

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“…However, they suffer from defects of the double bonds: (1) E/Z isomers with different properties in photophysics and sensing are formed during syntheses. The isomers are very hard to separate and thus they are often used in a mixture, leading to a complicated situation of mechanistic understanding; [13] (2) many double-bond containing AIEgens, such as Schiff bases and dicynao-substituted compounds, are vulnerable to nucleophiles, such as CN − , HS − , and OH − , [14] making them chemically unstable; and (3) the double bonds easily undergo photoisomerization and photooxidization upon excitation, leading to low photostability. [12] On the other hand, Type II AIEgens are of well-defined structures with more clear AIE mechanism, better chemical and photostabilities.…”

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confidence: 99%

Multifunctional AIEgens: Ready Synthesis, Tunable Emission, Mechanochromism, Mitochondrial, and Bacterial Imaging

Jiang

Kwok

et al. 2017

Adv Funct Materials

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Luminogens with aggregation-induced emission characteristics (AIEgens) are intriguing due to its rapid expansion in various high-tech applications. However, there is still in high demand on the development of novel AIEgens with easy preparation and functionalization, stable structures, tunable emissions, and high quantum efficiency. In this contribution, three AIEgens based on diphenyl isoquinolinium (IQ) derivatives are reported. They can be facilely synthesized and possess high structural stability, favorable visible light excitation, large Stokes shifts, high quantum yields, tunable colors, and sufficient two-photon absorption of near-infrared light. Importantly, they exhibit multifunctionalities. They exhibit mechanochromic property, making them capable to be applied for rewritable papers. They can also be applied in mitochondrial imaging with high specificity, cell permeability, brightness, biocompatibility, and photostability. They are promising for the applications in evaluation of mitochondrial membrane potential and image-guided cancer cell ablation. Last, they are able to stain bacteria in a wash-free manner. All these intriguing results suggest such readily accessible and multifunctional diphenyl IQ-based AIEgens provide a new platform for construction of advanced materials for practical applications.

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confidence: 99%

Multifunctional AIEgens: Ready Synthesis, Tunable Emission, Mechanochromism, Mitochondrial, and Bacterial Imaging

Jiang

Kwok

et al. 2017

Adv Funct Materials

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“…[ 22 ] This unusual phenomenon has been extensively explored and shown a wide range of applications, such as fl uorescence sensors, biological probes, and organic light-emitting diodes (OLEDs). [23][24][25][26][27] It is noteworthy that the morphology and crystallinity of the aggregated state of AIE molecules gave significant effects on their optical and electronic properties. [ 28,29 ] The organic AIE units can be controlled to assemble to various structures such as nanoparticles, nanowires and nanoplates, via different strategies.…”

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confidence: 99%

Single‐Layer Transition Metal Dichalcogenide Nanosheet‐Assisted Assembly of Aggregation‐Induced Emission Molecules to Form Organic Nanosheets with Enhanced Fluorescence

Tan

Huang

et al. 2013

Advanced Materials

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Single-layer transition metal dichalcogenide nanosheets including MoS2, TiS2 and TaS2 can be used as novel platforms for the fluorescence enhancement of aggregation-induced emission fluorophores. The small organic AIE unit can be assembled into a two-dimensional sheet structure via a simple precipitation technique assisted by these monolayers. The resultant organic sheets possess a size of 0.2-2 μm and a thickness of 9-20 nm.

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“…Chen et al [10]. Upon diffusing into cells, the sensor had been successfully applied for pH i imaging and monitoring with the use of confocal microscopy.…”

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confidence: 99%

Vibration-assisted optical injection of a single fluorescent sensor into a target cell

Liu

Maruyama

Masuda

et al. 2015

Sensors and Actuators B: Chemical

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Please cite this article as: H. Liu, H. Maruyama, Vibration-assisted optical injection of a single fluorescent sensor into a target cell, Sensors and Actuators B: Chemical (2015), http://dx.A c c e p t e d M a n u s c r i p t Highlights:  Selective adhesion and rapid injection of a fluorescent sensor into a target cell is proposed.  Multi-fluorescent sensors are encapsulated in liposome layers containing photochromic material for zeta potential control. Local vibration stimulus drove by optical tweezers is applied on the single sensor for rapid injection. With vibration stimulus, the sensor is successfully injected into cytoplasm in 30 min with a high injection rate of 80%. AbstractIn this paper, we propose the selective adhesion and rapid injection of a fluorescent sensor into a target cell via the optical control of zeta potential and local vibration stimulus using optical tweezers. A multi-fluorescent sensor, which can respond to both temperature and pH, was encapsulated in anionic lipid layers containing a photochromic material (spiropyran) via the layer-by-layer method. The zeta potential of the lipid layers containing spiropyran was adjusted from negative to positive by photo-isomerization of spiropyran using UV illumination. A single sensor was manipulated by optical tweezers and transferred to a cell surface, thereafter adhering selectively to the cell surface under UV illumination without excess sensor adhesion. We then drove the focal point of the optical tweezers to move up and down circularly near the sensor, mimicking a vibration on the sensor or rapid injection. The surface zeta potential of the liposome layers was measured using a zeta potential analyzer. The fluorescence resonance energy transfer (FRET) method was used to observe the changes in contact area between the adhered sensor and cell membrane before and after vibration. Holographic optical tweezers (HOT) and laser confocal microscopy were used to manipulate the single sensor and to capture Page 3 of 33 A c c e p t e d M a n u s c r i p t fluorescent images. The results showed that the vibration applied on the sensor could push down the sensor, inducing a downward displacement. This displacement caused a corresponding deformation of the cell membrane, which increased the contact area between the sensor and the cell membrane. Without vibration, the sensor was injected into the cytoplasm in 5 h at an injection rate of 40%. By applying the vibration stimulus, we succeeded in the rapid injection of the sensor in 30 min at an injection rate of 80%.

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Full-Range Intracellular pH Sensing by an Aggregation-Induced Emission-Active Two-Channel Ratiometric Fluorogen

Cited by 401 publications

References 23 publications

Multifunctional AIEgens: Ready Synthesis, Tunable Emission, Mechanochromism, Mitochondrial, and Bacterial Imaging

Multifunctional AIEgens: Ready Synthesis, Tunable Emission, Mechanochromism, Mitochondrial, and Bacterial Imaging

Single‐Layer Transition Metal Dichalcogenide Nanosheet‐Assisted Assembly of Aggregation‐Induced Emission Molecules to Form Organic Nanosheets with Enhanced Fluorescence

Vibration-assisted optical injection of a single fluorescent sensor into a target cell

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