Full-length transcriptome sequencing reveals the molecular mechanism of monoterpene and sesquiterpene biosynthesis in Cinnamomum burmannii

Abstract

Essential oil of Cinnamomum burmannii is rich in monoterpenes and sesquiterpenes and is widely used in cosmetics and medicines. Knowledge about the enzymes that catalyze the formation of monoterpenes and sesquiterpenes in C. burmannii is insufficient. Therefore, anatomy observation of C. burmannii at the four developmental stages (7 days, CBS1; 14 days, CBS2; 21 days, CBS3, and 28 days, CBS4) were conducted to elucidate the origins of essential oil production. Twelve full-length transcriptomes of C. burmannii leaves at the four stages were generated using Oxford Nanopore Technologies. GC-MS analysis revealed 15 monoterpene and sesquiterpenes dramatically increased from CBS1 to CBS4. A weighted correlation network analysis (WGCNA) in association and differentially expressed genes across four developmental stages were performed. A total of 44 differentially expressed genes (DEGs) were involved in terpenoid syntheses during leaf development. Among them, the DEGs of the mevalonate acid (MVA) pathway were predominantly expressed at CBS1, while those of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway showed increased expression from CBS2 to CBS4. Besides, fourteen genes were associated with monoterpene synthesis and nine with sesquiterpene synthesis. Functions of these DEGs were further predicted with regard to gene expression profile and phylogenetic relationship with those characterized in previous studies. In addition, 922 long noncoding RNAs (lncRNAs) were detected, of which twelve were predicted to regulate monoterpene and sesquiterpene biosynthesis. The present study provided new insights the molecular mechanisms of monoterpenoid and sesquiterpenoid syntheses of C. burmannii.

Reference Genes Selection and Validation for Cinnamomum burmanni by Real-Time Quantitative Polymerase Chain Reaction

“…Mature leaves from different borneol clones of C. burmannii (Cb-H, 51.96%; Cb-M, 27.65%; and Cb-L, 0.00%) were collected. Leaves at different developmental stages (Cb-S1, Cb-S2, Cb-S3 and Cb-S4) were collected from the same material, in accordance with our previous research [27]. All the samples were frozen immediately in liquid nitrogen and stored at −80 • C and all the treatments were conducted in triplicate.…”

“…In particular, the borneol-type essential oil is an important raw material for cosmetics and medicine due to the better permeability and antibacterial properties of borneol [23]. In the past few decades, the studies of C. burmannii mainly focused on the extraction, composition analysis of the compounds and biological activity [21,[24][25][26], but there is only limited research on gene regulation [27][28][29]. To some extent, this hindered the genetic improvement of the crop, and understanding the biological function of this crop is very important for further molecular breeding.…”

Potensi Antibakteri Minuman Fungsional Tradisional Jawa (Wedang Uwuh) Berdasarkan Variasi Waktu Rebusan

“…Senyawa bioaktif yang terdapat dalam kayu manis ini memiliki potensi sebagai antibakteri dan antihiperglikemia (Ginting et al, 2021). Daun kayu manis juga mengandung alkaloid, flavonoid, fenilik hidrokuinon, saponin, dan tanin (Hou et al, 2023). Daun kayu manis mengandung senyawa-senyawa aktif seperti eugenol, sinamaldehid, dan asam sinamat yang memiliki sifat antibakteri.…”

Identification of key genes controlling monoterpene biosynthesis of Citral-type Cinnamomum bodinieri Levl. Based on transcriptome and metabolite profiling

“…RNA-Seq technology was widely used in mining functional genes, probing active ingredient metabolic pathways and identifying key enzyme genes in Cinnamomum camphora (C. camphora) [18][19][20][21], C. burmannii [19]. Metabolites are the phenotypes in the central dogma of molecular biology, which can be regard as a fingerprint of the joint action of genes and the environment [22,23].…”