Full-genome analysis and pathogenicity of a genetically distinct Russian PRRSV-1 Tyu16 strain

Yuzhakov, Anton G.; Raev, S.A.; Щетинин, А. М.; Гущин, В.А.; Alekseev, Konstantin P.; Stafford, Viсtoria V.; Komina, A.K.; Zaberezhny, Alexei D.; Gulyukin, Alexey; Алипер, Т.И.

doi:10.1016/j.vetmic.2020.108784

Cited by 11 publications

(11 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An in-depth genetic and phenotypic characterization of emerging PRRSV isolates is also pivotal for the control and update of diagnostic methods, the monitoring of vaccine efficacy against new isolates, and also potential changes in virus pathogenicity and virulence. In general, the virulence of different PRRSV isolates was mainly evaluated for respiratory disease in young pigs ( 6 , 13 , 26 , 36 , 54 , 55 ). Additionally, challenge experiments investigating the mechanism of PRRSV-induced reproductive failure were predominately performed with PRRSV-2 ( 41 , 56 – 58 ).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to the other strains, no experimental infection trial was conducted ( 14 ). c According to the authors of the cited publication, Tyu16 belongs to PRRSV-1 with considerable distance to other PRRSV-1 strains ( 36 ). In ORF7, this isolate forms a monophyletic group with subtype 1 strains, whereas in ORF5 there is a grouping with subtype 2 strains.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Phenotypic Characterization of a Virulent PRRSV-1 Isolate in a Reproductive Model With and Without Prior Heterologous Modified Live PRRSV-1 Vaccination

Kreutzmann

Stadler²,

Knecht

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Reproductive disorders induced by porcine reproductive and respiratory syndrome virus (PRRSV) cause high economic losses in the pig industry worldwide. In this study, we aimed to phenotypically characterize a virulent PRRSV-1 subtype 1 isolate (AUT15-33) in a reproductive model. Furthermore, the protective effect of a heterologous modified live virus vaccine (ReproCyc® PRRS EU) was evaluated. In addition, PRRSV AUT15-33 was genotypically compared to other well-characterized isolates. Sixteen gilts were equally divided into four groups: a vaccinated and infected group (V–I), a vaccinated and non-infected group (V–NI), a non-vaccinated and infected group (NV–I), and a non-vaccinated and non-infected (NV–NI) group. After PRRSV infection on gestation day 84, all gilts were clinically examined on a daily basis, and blood samples were taken at five timepoints. Necropsy was performed 3 weeks after infection. The fetal preservation status was assessed, and PRRSV RNA concentrations were measured in the blood and tissue samples from all gilts and fetuses. After infection, all four gilts in the NV–I group were viremic throughout 17 days post-infection (dpi), whereas two gilts in the V–I group were viremic at only one timepoint at 6 dpi. The viral load was significantly higher in gilt serum, tracheobronchial lymph nodes, uterine lymph nodes, maternal endometrium, and fetal placenta of NV–I gilts compared to the V–I ones (p < 0.05). Moreover, the preservation status of the fetuses derived from NV–I gilts was significantly impaired (55.9% of viable fetuses) compared to the other groups (p < 0.001). Upon comparison with other known isolates, the phylogenetic analyses revealed the closest relation to a well-characterized PRRSV-1 subtype 1 field isolate from Belgium. In conclusion, the high virulence of AUT15-33 was phenotypically confirmed in an experimental reproductive model. The vaccination of the gilts showed promising results in reducing viremia, fetal damage, and transplacental transmission of the PRRSV-1 strain characterized in this study.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Phenotypic Characterization of a Virulent PRRSV-1 Isolate in a Reproductive Model With and Without Prior Heterologous Modified Live PRRSV-1 Vaccination

Kreutzmann

Stadler²,

Knecht

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…Severe disease is unusual with PRRSV1-1 variants. Historically, the subtype 1 viruses are typically of lower pathogenicity [25]. Without sequencing, the system would have been unaware that it was struggling with two different outbreaks caused by the same viral genus.…”

Section: Discussionmentioning

confidence: 99%

A Molecular and Epidemiological Description of a Severe Porcine Reproductive and Respiratory Syndrome Outbreak in a Commercial Swine Production System in Russia

Havas

Makau

Шаповалов³

et al. 2022

Viruses

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating disease of swine in many parts of the world. Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 is endemic in Europe, and prevalence of the subtypes differ spatially. In this study, we investigated a severe PRRS outbreak reported in 30 farms located in eastern Russia that belong to a large swine production company in the region that was also experiencing a pseudorabies outbreak in the system. Data included 28 ORF5 sequences from samples across 18 of the 25 infected sites, reverse transcriptase real-time polymerase chain reaction (RT-qPCR) results from diagnostic testing, reports of clinical signs, and animal movement records. We observed that the outbreak was due to two distinct variants of wildtype PRRSV type 1 subtype 1 with an average genetic distance of 15%. Results suggest that the wildtype PRRSV variants were introduced into the region around 2019, before affecting this production system (i.e., sow farms, nurseries, and finisher farms). Clinical signs did not differ between the variants, but they did differ by stage of pig production. Biosecurity lapses, including movement of animals from infected farms contributed to disease spread.

show abstract

“…Thus, the detection of a new member of PRRSV1-2 in the present study offers additional proof of the wide distribution of this genotype in Russia. Isolates of PRRSV1-1 (including the so-called Russian group of viruses), PRRSV1-2 , and PRRSV1-3 differ significantly in pathogenicity [ 11 , 12 , 19 , 20 ]. Marked differences in the nucleotide sequence between PRRSV1-2 isolates and PRRSV1 vaccine strains, which all belong to PRRSV1-1, including differences in the ORF5 sequence (which encodes a major epitope: the envelope glycoprotein), could potentially hamper vaccine efficacy [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Russia, PRRS is mainly caused by PRRSV1-1 (including the so-called atypical Russian group of viruses) and PRRSV1-2 [ 9 , 11 , 12 ]. Only limited data are available on PRRSV2 epidemiology in Russia.…”

Section: Introductionmentioning

confidence: 99%

An Outbreak of a Respiratory Disorder at a Russian Swine Farm Associated with the Co-Circulation of PRRSV1 and PRRSV2

Raev

Yuzhakov

Bulgakov

et al. 2020

Viruses

View full text Add to dashboard Cite

We conducted a cross-sectional study to identify the major respiratory pathogen responsible for an outbreak of respiratory disease at a swine farm in West Siberia in 2019. We discovered that the peak of morbidity and mortality coincided with a high level of porcine reproductive and respiratory syndrome virus (PRRSV) 1 and 2-related viremia. Based on longer PRRSV2 viremia, the dominant role of PRRSV2 over PRRSV1 in the outbreak was assumed. Phylogenetic analysis revealed that the PRRSV1 strain belonged to sub-genotype 2—one of the predominant groups of genotype 1 PRRSVs in Russia. A partial open reading frame 7 sequence of the PRRSV2 isolate demonstrated a high identity with modified live vaccine-related strains from Denmark (93%) and wild-type VR2332 (92%). We identified the first instance of PRRSV1/PRRSV2 mixed infection in Russia. This finding indicates that further field investigations are needed to access PRRSV2 epidemiology in eastern Europe.

show abstract

Full-genome analysis and pathogenicity of a genetically distinct Russian PRRSV-1 Tyu16 strain

Cited by 11 publications

References 33 publications

Phenotypic Characterization of a Virulent PRRSV-1 Isolate in a Reproductive Model With and Without Prior Heterologous Modified Live PRRSV-1 Vaccination

Phenotypic Characterization of a Virulent PRRSV-1 Isolate in a Reproductive Model With and Without Prior Heterologous Modified Live PRRSV-1 Vaccination

A Molecular and Epidemiological Description of a Severe Porcine Reproductive and Respiratory Syndrome Outbreak in a Commercial Swine Production System in Russia

An Outbreak of a Respiratory Disorder at a Russian Swine Farm Associated with the Co-Circulation of PRRSV1 and PRRSV2

Contact Info

Product

Resources

About