Full characterization of an IncR plasmid harboring qnrS1 recovered from a VIM-11-producing Pseudomonas aeruginosa

Elena, Alan Xavier; Quinteros, Mirta; Conza, José Alejandro Di; Gutkind, Gabriel; Cejas, Daniela; Radice, Marcela

doi:10.1016/j.ram.2019.12.001

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…Thus, such plasmids play an important role in the dissemination of resistances against last resort antimicrobials. Furthermore, several IncX3 plasmids have been reported to carry qnrB, qnrS and bla genes [41,42,44,45], highlighting the importance of this plasmid for the dissemination of antimicrobial resistance genes in Enterobacteriaceae [37]. In contrast to the published results, the majority of our IncX3 plasmids of this study lacked the IS6 transposase gene.…”

Section: Characteristics Of Plasmids Assigned To the Reference Plasmid Pkpvst101_6contrasting

confidence: 89%

“…In general, it has been shown that this plasmid type is able to be spread to different species of Enterobacteriaceae. Although qnrS1-IncR plasmids had been described before [45], the combined presence of IncX and IncR seems to be rare, as we could not find any description in the current literature.…”

Section: Characteristics Of the Plasmid Assigned To The Unnamed Reference Plasmid Of The Strain R1701mentioning

confidence: 58%

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Dissection of Highly Prevalent qnrS1-Carrying IncX Plasmid Types in Commensal Escherichia coli from German Food and Livestock

et al. 2021

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Plasmids are mobile genetic elements, contributing to the spread of resistance determinants by horizontal gene transfer. Plasmid-mediated quinolone resistances (PMQRs) are important determinants able to decrease the antimicrobial susceptibility of bacteria against fluoroquinolones and quinolones. The PMQR gene qnrS1, especially, is broadly present in the livestock and food sector. Thus, it is of interest to understand the characteristics of plasmids able to carry and disseminate this determinant and therewith contribute to the resistance development against this class of high-priority, critically important antimicrobials. Therefore, we investigated all commensal Escherichia (E.) coli isolates, with reduced susceptibility to quinolones, recovered during the annual zoonosis monitoring 2017 in the pork and beef production chain in Germany (n = 2799). Through short-read whole-genome sequencing and bioinformatics analysis, the composition of the plasmids and factors involved in their occurrence were determined. We analysed the presence and structures of predominant plasmids carrying the PMQR qnrS1. This gene was most frequently located on IncX plasmids. Although the E. coli harbouring these IncX plasmids were highly diverse in their sequence types as well as their phenotypic resistance profiles, the IncX plasmids-carrying the qnrS1 gene were rather conserved. Thus, we only detected three distinct IncX plasmids carrying qnrS1 in the investigated isolates. The IncX plasmids were assigned either to IncX1 or to IncX3. All qnrS1-carrying IncX plasmids further harboured a β-lactamase gene (bla). In addition, all investigated IncX plasmids were transmissible. Overall, we found highly heterogenic E. coli harbouring conserved IncX plasmids as vehicles for the most prevalent qnr gene qnrS1. These IncX plasmids may play an important role in the dissemination of those two resistance determinants and their presence, transfer and co-selection properties require a deeper understanding for a thorough risk assessment.

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Section: Characteristics Of Plasmids Assigned To the Reference Plasmid Pkpvst101_6contrasting

confidence: 89%

Section: Characteristics Of the Plasmid Assigned To The Unnamed Reference Plasmid Of The Strain R1701mentioning

confidence: 58%

Dissection of Highly Prevalent qnrS1-Carrying IncX Plasmid Types in Commensal Escherichia coli from German Food and Livestock

et al. 2021

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“…Este tipo de características determina a P. mirabilis más como un agente oportunista que como uno patógeno. De igual forma, la cantidad de secuencias proteicas de codificación en el genoma se encuentra ligado a la capacidad selectiva de la célula de incluir marcadores de resistencia a antimicrobianos (Elena et al, 2020).…”

Section: Total Cepas 17unclassified

Identificación de Proteus mirabilis en carne de pollo procedente de comercios en Cantón Ambato

Cortez Guerra,

Escobar Aguilar,

Gómez Usiña

et al. 2023

Pentaciencias

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Proteus mirabilis es una bacteria capaz de causar patologías de interés en salud pública. Se presenta de forma común en la carne de pollo. P. mirabilis no destaca por sus niveles de patogenicidad, pero sí por su difícil abordaje terapéutico una vez desarrollada la infección, debido a la resistencia a antibióticos que ha presentado. En este estudio se recolectaron 135 muestras de carne de pollo en distintos centros de expendio de Ambato (45 formales, 45 informales y 45 camales). Se sembró en medios de cultivo selectivo (Agar McConkey y Agar XLD), se usó Tinción de Gram y se realización pruebas bioquímicas (Prueba Oxidasa y TSI) e identificación molecular mediante la amplificación de la región 16S del ADN ribosomal, resultando 48 muestras de P. mirabilis con 17 cepas diferentes, siendo la más prevalente NCTC 11938. P. mirabilis se identificó sobre todo en centros de expendio formales, seguido por camales y centros de expendio informales. Esta contaminación se relaciona con el incumplimiento de protocolos de procesamiento de alimentos. La gran prevalencia de esta bacteria representa una problemática de interés público, por los peligros que se relacionan a la contaminación de la carne con ella.

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“…Thereby, locally adapted populations of pathogenic bacteria can, under the selective pressure of antibiotics, acquire multiple antibiotic resistances. In recent studies, plasmid-borne carbapenemases such as bla GPC-1 , bla VIM-1 , bla VIM-4 , bla VIM-11 , bla GIM , bla SIM , bla I MP-13 and bla NDM-type have been reported as causes of carbapenem resistance in P. aeruginosa in Germany, 8 Egypt, 9 Algeria, 10 Argentina, 11 UK, 12 India 13 and South Korea. 14 , 15 …”

Section: Introductionmentioning

confidence: 99%

High incidence of carbapenemase-producingPseudomonas aeruginosaclinical isolates from Lagos, Nigeria

Olalekan

Bader

Iwalokun

et al. 2023

JAC-Antimicrobial Resistance

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Background Carbapenem-resistant Pseudomonas aeruginosa strains are on the rise worldwide. This study characterized clinical isolates of P. aeruginosa from three Nigerian hospitals for carbapenem resistance. Methods Strains isolated from wounds (n = 88), urine/catheter tips (n = 25), sputum/tracheotomy aspirates (n = 5), ear swabs (n = 4) and vaginal swabs (n = 1) were identified by MALDI-TOF and antibiotic susceptibility testing was performed using the VITEK 2 system. The genomic DNA of each isolate was subject to sequencing using Illumina and Oxford nanopore technology. Bioinformatics analyses were performed to detect antimicrobial resistance genes, clonal affiliations and phylogenetic relations of 123 non-duplicate P. aeruginosa isolates, whereas assembly of the nanopore reads using the plasmIDent pipeline enabled the identification of plasmids. Results Forty-three percent of the isolates were resistant to all antibiotic categories tested. More than 40% of the isolates were resistant to the carbapenems imipenem and/or meropenem (39% and 44%, respectively). Among the meropenem-resistant isolates, 48 (89%) carried at least one carbapenemase gene. The predominant one was blaNDM-1 (n = 34), which conferred resistance to all five antibiotic categories and highly increased the MICs of both meropenem and imipenem. The other recurrent carbapenemase genes were blaVIM-2 (n = 4), and blaVIM-5-like (n = 11), which co-existed with blaNDM-1 in two isolates. Conclusions The study revealed a high rate of carbapenem resistance and conjugative, broad host range plasmids carrying carbapenemase-encoding genes, especially the NDM-1 type, among isolates of P. aeruginosa. This may forebode the emergency of ubiquitous carbapenem resistance urging the implementation of infection control and antimicrobial stewardship strategies in Nigerian hospitals.

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Full characterization of an IncR plasmid harboring qnrS1 recovered from a VIM-11-producing Pseudomonas aeruginosa

Cited by 6 publications

References 12 publications

Dissection of Highly Prevalent qnrS1-Carrying IncX Plasmid Types in Commensal Escherichia coli from German Food and Livestock

Dissection of Highly Prevalent qnrS1-Carrying IncX Plasmid Types in Commensal Escherichia coli from German Food and Livestock

Identificación de Proteus mirabilis en carne de pollo procedente de comercios en Cantón Ambato

High incidence of carbapenemase-producingPseudomonas aeruginosaclinical isolates from Lagos, Nigeria

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