FUCOSYLATED Α-Fetoprotein AS MARKER OF EARLY HEPATOCELLULAR CARCINOMA

Aoyagi, Yoshinobu; Isemura, Mamoru; Suzuki, Yuzuru; Sekine, Chuichi; Soga, Kenji; Ozaki, Toshihiko; Ichida, Fumihiro

doi:10.1016/s0140-6736(85)92643-1

Cited by 72 publications

(46 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The molecular basis for this LCA-reactive species of AFP is fucosylation of the sugar chain (Aoyagi et al, 1984(Aoyagi et al, , 1985a(Aoyagi et al, , b, 1986 (Palmer & Wolfe, 1976;Palmer et al, 1980;Ord6fiez & Manning, 1984) that immunoreactive AAT is present in HCC cells by the immunoperoxidase method, it was suggested that HCC cells might produce AAT, of an abnormal type i.e., that the change in the reactivity of AAT with LCA occurs in association with neoplastic transformation of hepatocytes, irrespective of serum AAT concentration. During the followup of the patients with chronic liver diseases, measurement of LCA-reactive species of AAT might thus be useful for the diagnosis of HCC, especially when serum AFP concentration is within the normal range.…”

Section: Discussionmentioning

confidence: 99%

“…The LCA-reactive species of AFP is known to contain a carbohydrate chain of the fucosylated biantennary complex type and most of the patients with HCC are known to have an elevated serum concentration of fucosylated AFP (Aoyagi et al, 1984(Aoyagi et al, , 1985a(Aoyagi et al, ,b, 1986. Since a carbohydrate structure of AAT is similar to that of AFP (Mega et al, 1980), LCAreactive species of AAT is supposed to be a fucosylated fraction.…”

Section: Discussionmentioning

confidence: 99%

“…The measurement of serum alpha-foetoprotein (AFP) concentration has been used extensively for the detection of HCC. Furthermore, our previous studies have shown that measurement of the fucosylated fraction of AFP is very useful for the early diagnosis of HCC and that it can distinguish an AFP species due to malignancy from one due to benign liver diseases (Aoyagi et al, 1984(Aoyagi et al, , 1985a(Aoyagi et al, ,b, 1986. There are, however, also HCCs, the corresponding serum AFP concentrations of which are within the normal range.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The reactivity of alpha-1-antitrypsin with Lens culinaris agglutinin and its usefulness in the diagnosis of neoplastic diseases of the liver

Sekine

Aoyagi²,

Suzuki³

et al. 1987

Br J Cancer

View full text Add to dashboard Cite

Summary The reactivity of alpha-l-antitrypsin (AAT) with Lens culinaris agglutinin (LCA) was studied by crossed immuno-affinity electrophoresis of the sera of 246 subjects from 6 groups (acute virus hepatitis, chronic hepatitis, liver cirrhosis, hepatocellular carcinoma (HCC), carcinoma metastatic to the liver and normal controls). Two species of AAT (LCA-reactive and -nonreactive species) were detected on crossed immuno-affinity electrophoresis in a gel containing LCA. The percentages of LCA-reactive species of AAT in neoplastic diseases of the liver were significantly higher than those in benign liver diseases and normal controls. There was no correlation between the percentage of LCA-reactive species of AAT and serum AAT concentration in any group. Furthermore, in studying 15 pairs of serum samples before and after the subsequent development of HCC, the percentage of LCA-reactive species of AAT after HCC occurrence was significantly higher than that before, although there was no statistically significant difference between the serum AAT concentration before and after development of the disease. The latter 15 patients were all of the normal protease inhibitor phenotype (PiMM) and no change in phenotype was observed before and after the development of HCC. The results indicate that measurement of the reactivity of AAT with LCA can be a useful marker for the diagnosis of HCC and carcinoma metastatic to the liver, especially when serum concentrations of alpha-foetoprotein or other tumour markers are within the normal ranges.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

The reactivity of alpha-1-antitrypsin with Lens culinaris agglutinin and its usefulness in the diagnosis of neoplastic diseases of the liver

Sekine

Aoyagi²,

Suzuki³

et al. 1987

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Fucosylated alpha-fetoprotein (AFP), which is named the AFP-L3 fraction after the measurement method used, has been clinically used as a specific cancer biomarker for hepatocellular carcinoma (HCC) since 1996 in Japan and 2005 in the United States (14,15). In addition, advanced large-scale analytical methods involving a fucose-recognizing lectin for human serum glycoproteome recently revealed glycoprotein 73 (GP73) is a novel cancer biomarker for HCC (16).…”

Section: Cancer and Fucosylationmentioning

confidence: 99%

Roles of Fucosylation in Tumor Immunology

Moriwaki

Miyoshi

2010

TIGG

View full text Add to dashboard Cite

The explosive progress of glycobiology in the past few decades has revealed that fucose is indispensable in various organisms and is involved in many biological events. Many researchers have spent a lot of time in order to unveil the mysterious functions of fucosylation. However, the findings which have ever been clarified are not sufficient to explain all fucosylation-related biological events. In the field of oncology, although several fucosylated molecules have been reported to be increased in cancer and have been used as cancer biomarkers, it remains unknown how dynamic changes of fucosylation are associated with cancer biology. Recently, we found that fucosylation is involved in NK cell-mediated tumor immune surveillance through regulation of the susceptibility to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is an apoptosis-inducing molecule. In this review, we describe the relationship between cancer biology and fucosylation, according to our recent findings. A. IntroductionGlycosylation comprises the enzymatic attachment of sugars to biological macromolecules, such as proteins and lipids, and is one of the most important post-translational modifications. Fucose is a kind of monosaccharide that forms oligosaccharides through binding via glycosidic linkages. L-fucose is equivalent to 6-deoxy-L-galactose and exhibits two structural differences from other hexoses in mammals. One is the lack of a hydroxyl group on the carbon at the 6-position and the other is the L-configuration. Fucosylation is a reaction by which an L-fucose residue is attached to oligosaccharides on glycoproteins and glycolipids or proteins. Fucosylation can confer unique functional properties to oligosaccharides or proteins and is closely associated with various physiological events, including development, incompatible blood transfusion reaction, and leukocyte trafficking (1). In addition,

show abstract

“…Several galactohydrolase was from Boeringer Mannheim Bioinvestigators have reported heterogeneous reactivity of AFP chemica (W. Germany). with lectins (Smith et al, 1977;Bayard & Kerckaert, 1977;Miyazaki et al, 1981; Monoclonal anti-AFP antibodies Breborowicz et al, 1981;Taketa et al, 1983) (Aoyagi et al, 1984(Aoyagi et al, , 1985a(Aoyagi et al, , 1986 (Aoyagi et al, 1984). Fucosylation of the sugar chain is the isolated immunoglobulins were pure as revelaed by sodium molecular basis for this variation of AFP (Aoyagi et al, dodecyl sulphate polyacrylamide gel electrophoresis andim1985a,b, 1986).…”

Section: Alpha-foetoprotein (Afp) Is One Of the Major Plasmamentioning

confidence: 99%

Close topographical relationship in alpha foetoprotein (AFP) between a lens culinaris binding glycan and the epitope recognized by AFP-reactive monoclonal antibody, 18H4

Suzuki¹,

Aoyagi²,

Muramatsu³

et al. 1987

Br J Cancer

View full text Add to dashboard Cite

Summary Monoclonal antibodies 18H4 and 19F12 against alpha-foetoprotein (AFP) were examined by enzyme immunoassay for binding to two forms of AFP that were separated on the basis of the reactivity with lentil lectin (LCA). LCA-binding and LCA non-binding AFP, coated on a solid phase, reacted with 18H4 but reactivity with the LCA-binding species was inhibited by 60% following pretreatment of the AFP with LCA. The lectin was a very poor inhibitor of binding of 18H4 to the AFP which did not interact with LCA. In an alternative binding assay, a polyclonal anti-AFP coated solid phase was reacted with P-galactosidase-labelled 18H4. Pre-treatment with LCA of the LCA-reactive AFP gave 56% inhibition of binding of conjugated 18H4 while little inhibition was achieved with the LCA-nonreactive AFP component. These findings show that the epitope recognised by 18H4 is distinct from the glycan sequence that is reactive with LCA. However, the LCA-binding oligosaccharides occur in close proximity to the 18H4 epitope in the native AFP.

show abstract

FUCOSYLATED Α-Fetoprotein AS MARKER OF EARLY HEPATOCELLULAR CARCINOMA

Cited by 72 publications

References 1 publication

The reactivity of alpha-1-antitrypsin with Lens culinaris agglutinin and its usefulness in the diagnosis of neoplastic diseases of the liver

The reactivity of alpha-1-antitrypsin with Lens culinaris agglutinin and its usefulness in the diagnosis of neoplastic diseases of the liver

Roles of Fucosylation in Tumor Immunology

Close topographical relationship in alpha foetoprotein (AFP) between a lens culinaris binding glycan and the epitope recognized by AFP-reactive monoclonal antibody, 18H4

Contact Info

Product

Resources

About