Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation

El-Far, Yousra M.; Khodir, Ahmed E.; Emarah, Ziad; Ebrahim, Mohamed A.; Al‐Gayyar, Mohammed M.H.

doi:10.1080/01635581.2020.1798478

Cited by 14 publications

(9 citation statements)

References 36 publications

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“…Quantitative real-time polymerase chain reaction (RT–PCR) . The PCR analysis was performed as described previously by our group [ 4 ]. The sequence of Nrf2 the forward primer 5′-GAGACGGCCATGACTGAT-3′ and reverse primer, 5′-GTGAGGGGATCGATGAGTAA-3′ and for GAPDH, the forward primer 5′- CCATCAACGACCCCTTCATT-3′ and reverse primer 5′- CACGACATACTCAGCACCAGC-3′.…”

Section: Methodsmentioning

confidence: 99%

“…HCC can result from exposure to numerous factors, including chronic hepatitis C or hepatitis B viral infection, high alcohol consumption and diabetes [ 2 ]. HCC is an inflammatory disease, with more than 90% of patients with HCC developing chronic liver damage [ 3 , 4 ]. The prognosis of HCC is poor due to the high rate of metastasis and recurrence of the disease [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the aim of the present study was to assess the chemopreventive and hepatoprotective effects of genistein in HCC via investigating its effect on the versican/PDGF/PKC and ERK signaling pathways. For induction of HCC in rats, thioacetamide was used [ 3 , 4 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemopreventive and hepatoprotective effects of genistein via inhibition of oxidative stress and the versican/PDGF/PKC signaling pathway in experimentally induced hepatocellular carcinoma in rats by thioacetamide

et al. 2022

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Objective Genistein is a recognized isoflavone present in soybeans with antioxidant, anti-inflammatory, antiangiogenic and antitumor activities. This study aimed to test ability of genistein in modulating versican/platelet derived growth factor (PDGF) axis in HCC. Methods HCC was experimentally induced in male Sprague-Dawley rats then treated with 25 or 75 mg/kg genistein. Antioxidant activities of genistein was assessed by measuring the gene expression of Nrf2 and the hepatic levels of malondialdehyde (MDA), superoxide dismutase (SOD) and reduced glutathione. Expression of versican, PDGF, protein kinase C (PKC) and ERK-1 protein was assessed by Western blotting and immunostaining. Results HCC induced an elevation in oxidative stress, PDGF, versican, PKC and ERK protein expression levels. Genistein significantly reduced an HCC-induced increase in oxidative stress. Moreover, genistein dose-dependently reduced HCC-induced elevation of PDGF, versican, PKC and ERK protein expression levels. Moreover, genistein helped retain a normal hepatocyte structure and reduced fibrous tissue deposition, especially in high dose. Conclusions Genistein exerted antitumor and antioxidant effects and therefore suppress HCC development via inhibition of the PDGF/versican bidirectional axis, suppressing both ERK1 and PKC as downstream regulators. Therefore, genistein is a potential novel therapeutic candidate for improving the outcome of patients with HCC.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemopreventive and hepatoprotective effects of genistein via inhibition of oxidative stress and the versican/PDGF/PKC signaling pathway in experimentally induced hepatocellular carcinoma in rats by thioacetamide

et al. 2022

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“…Furthermore, in an in vivo model of hepatocellular carcinoma (HCC), fucoidan significantly improved survival, inhibited cellular proliferation, and decreased serum AFP expression levels in HCC rats. Additionally, fucoidan reduced the upregulation of miR-143 and NF-κB expression levels induced by HCC ( El-Far et al, 2021 ). The miR-143/NF-κB pathway was proven to be an important component involved in tumor progression and invasion ( Zheng et al, 2018 ).…”

Section: Ncrnas As Oncogenes or Tumor Suppressors Involved In The Ant...mentioning

confidence: 99%

Non-coding RNA-related antitumor mechanisms of marine-derived agents

et al. 2022

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In the last two decades, natural active substances have attracted great attention in developing new antitumor drugs, especially in the marine environment. A series of marine-derived compounds or derivatives with potential antitumor effects have been discovered and developed, but their mechanisms of action are not well understood. Emerging studies have found that several tumor-related signaling pathways and molecules are involved in the antitumor mechanisms of marine-derived agents, including noncoding RNAs (ncRNAs). In this review, we provide an update on the regulation of marine-derived agents associated with ncRNAs on tumor cell proliferation, apoptosis, cell cycle, invasion, migration, drug sensitivity and resistance. Herein, we also describe recent advances in marine food-derived ncRNAs as antitumor agents that modulate cross-species gene expression. A better understanding of the antitumor mechanisms of marine-derived agents mediated, regulated, or sourced by ncRNAs will provide new biomarkers or targets for potential antitumor drugs from preclinical discovery and development to clinical application.

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“…One of these natural compounds is fucoidan, which is sulfated polysaccharide of brown seaweed. It demonstrates various pharmacological actions such as anti-inflammatory, anti-tumor and anti-bacterial effects [ 11 ]. Moreover, it has been investigated in preclinical studies for immunomodulatory, anti-oxidant, anti-angiogenic, anti-viral and anti-hyperglycemic activities [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Curative effects of fucoidan on acetic acid induced ulcerative colitis in rats via modulating aryl hydrocarbon receptor and phosphodiesterase-4

Bagalagel

Diri

Noor

et al. 2022

BMC Complement Med Ther

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Background Ulcerative colitis (UC) is an inflammatory bowel disease. Fucoidan, sulfated polysaccharide of brown seaweed, demonstrates various pharmacological actions as anti-inflammatory, anti-tumor and anti-bacterial effects. Therefore, we opt to investigate the potential curative effects of fucoidan in experimentally induced UC in rats through modulating aryl hydrocarbon receptor (AhR), phosphodiesterase-4 (PDE4), nuclear factor erythroid 2-related factor 2 (Nrf2) and Heme Oxygenase-1 (HO-1). Methods UC was induced in rats using intracolonic 2 ml of 4% acetic acid. Some rats were treated with 150 mg/kg fucoidan. Samples of colon were used to investigate gene and protein expression of AhR, PDE4, Nrf2, HO-1 and cyclic adenosine monophosphate (cAMP). Sections of colon were stained with hematoxylin/eosin, Alcian blue or immune-stained with anti-PDE4 antibodies. Results Investigation of hematoxylin/eosin stained micro-images of UC rats revealed damaged intestinal glands, severe hemorrhage and inflammatory cell infiltration, while sections stained with Alcian Blue revealed damaged and almost absent intestinal glands. UC results in elevated gene and protein expression of PDE4 associated with reduced gene and protein expression of AhR, IL-22, cAMP, Nrf2 and HO-1. Finally, UC increased the oxidative stress and reduced antioxidant activity in colon tissues. All morphological changes as well as gene and protein expressions were ameliorated by fucoidan. Conclusion Fucoidan could treat UC induced in rats. It restored the normal weight and length of colon associated with morphological improvement as found by examining sections stained with hematoxylin/eosin and Alcian Blue. The curative effects could be explained by enhancing antioxidant activity, reducing the expression of PDE4 and increasing the expression of AhR, IL-22 and cAMP.

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Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation

Cited by 14 publications

References 36 publications

Chemopreventive and hepatoprotective effects of genistein via inhibition of oxidative stress and the versican/PDGF/PKC signaling pathway in experimentally induced hepatocellular carcinoma in rats by thioacetamide

Chemopreventive and hepatoprotective effects of genistein via inhibition of oxidative stress and the versican/PDGF/PKC signaling pathway in experimentally induced hepatocellular carcinoma in rats by thioacetamide

Non-coding RNA-related antitumor mechanisms of marine-derived agents

Curative effects of fucoidan on acetic acid induced ulcerative colitis in rats via modulating aryl hydrocarbon receptor and phosphodiesterase-4

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