Fuchsiella alkaliacetigena gen. nov., sp. nov., an alkaliphilic, lithoautotrophic homoacetogen from a soda lake

Abstract

The first alkaliphilic obligately anaerobic hydrogenotrophic homoacetogenic bacterium, strain Z-7100T, was isolated from sediments of the soda-depositing soda lake Tanatar III (Altay, Russia). Cells were thin, flexible rods, motile, Gram-negative and spore-forming. The organism was an obligate alkaliphile, growing at pH 8.5 to 10.5, with optimum growth at pH 8.8–9.3, and it grew in soda brines containing 1.9–4.7 M total Na+ (optimum at 2.8–3.3 M). It exhibited an obligate dependence upon sodium carbonate but not upon chloride ions with an NaCl range for growth of 0–14 % (w/v) and an optimum at 7.0–8.5 % (w/v). The isolate was mesophilic and grew at temperatures from 25 to 45 °C, with an optimum at 40 °C. An H2+CO2 mixture, ethanol, pyruvate and lactate were utilized with the formation of acetate as the sole metabolic product. Carbohydrates and amino acids did not support growth. The isolate had a respiratory type of metabolism, reducing NO 3 – , SeO4 2− or anthraquinone-2,6-disulfonate (as electron acceptors with ethanol as an electron donor). It was able to grow chemolithotrophically on H2+CO2 in medium supplemented with a vitamin solution only. The major cellular fatty acids were the saturated fatty acids anteiso-C15, C14 : 0 and C16 : 0 and the aldehydes C16, C14 and anteiso-C15. The DNA G+C content of the isolate was 32.0 mol%. 16S rRNA gene sequence analysis showed that strain Z-7100T is a member of the order Halanaerobiales and represents a new branch within the family Halobacteroidaceae , clustering with the type strain of Selenihalanaerobacter shriftii (92.9 % gene sequence similarity). On the basis of its physiological characteristics and phylogenetic position, the isolate is considered to represent a novel species in a new genus within the family Halobacteroidaceae . The name Fuchsiella alkaliacetigena gen. nov., sp. nov. is proposed. The type strain of the type species is Z-7100T ( = DSM 24880T = VKM B-2667T).

Coprobacter fastidiosus gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from infant faeces

“…Cellular fatty acids and menaquinones were determined in late exponential phase cultures grown at the optimal temperature in EG broth. Long-chain fatty acids were analysed using GC-MS. Fatty acid methyl esters were prepared by acid methanolysis of dry biomass and extracted as described previously (Zhilina et al, 2012) and processed on an AT-5850/5973 GC-MS system (Agilent Technologies) according to the method of Shcherbakova et al (2005). Respiratory quinones were detected following the procedure of Collins (1985).…”

Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

“…Cellular fatty acids and menaquinone profiles were analysed in the late exponential phase cultures in MRS broth. Longchain fatty acids were separated and detected using GC-MS according to Zhilina et al (2012). Respiratory quinones were detected following the procedure of Collins (1985).…”

Inmirania thermothiophila gen. nov., sp. nov., a thermophilic, facultatively autotrophic, sulfur-oxidizing gammaproteobacterium isolated from a shallow-sea hydrothermal vent

“…Cellular fatty acids composition was analysed by GC-MS according to Zhilina et al (2012). The genomic DNA G+C content was determined from the melting point according to Marmur & Doty (1962) with DNA of Escherichia coli K-12 as a reference.…”