FTIR spectroscopy as an analytical tool to compare glycosylation in therapeutic monoclonal antibodies

Derenne, Allison; Derfoufi, Kheiro-Mouna; Cowper, Ben; Delporte, Cédric; Goormaghtigh, Erik

doi:10.1016/j.aca.2020.03.038

Cited by 55 publications

(49 citation statements)

References 23 publications

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“…Here, the authors obtained valuable information from only the infrared spectrum of the glycoprotein, with no labelling or separation required to determine an accurate fingerprint of the glycosylation profile ( Fig. 5) [2]. Previous reports have shown contrasting relationships between protein synthesis rates and glycosylation of different proteins [73], however, glycosylation of the final product is certainly influenced by the host cell line, the cell culture and the purification process [70].…”

Section: In-linementioning

confidence: 92%

“…FTIR spectra of glycoproteins, displaying different spectral signatures, due to the sensitivity of spectra to minor changes in glycan and monosaccharide composition[2]. (Reprinted with permission from Derenne, A.; Derfoufi, K. M.; Cowper, B.; Delporte, C.; Goormaghtigh, E., FTIR spectroscopy as an analytical tool to compare glycosylation in therapeutic monoclonal antibodies.…”

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confidence: 99%

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ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

Tiernan

Byrne

Kazarian

2020

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

140

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Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a label-free, non-destructive technique that can be applied to a vast range of biological applications, from imaging cancer tissues and live cells, to determining protein content and protein secondary structure composition. This review summarises the recent advances in applications of ATR-FTIR spectroscopy to biopharmaceuticals, the application of this technique to biosimilars, and the current uses of FTIR spectroscopy in biopharmaceutical production. We discuss the use of ATR-FTIR spectroscopic imaging to investigate biopharmaceuticals, and finally, give an outlook on the possible future developments and applications of ATR-FTIR spectroscopy and spectroscopic imaging to this field. Throughout the review comparisons will be made between FTIR spectroscopy and alternative analytical techniques, and areas will be identified where FTIR spectroscopy could perhaps offer a better alternative in future studies. This review focuses on the most recent advances in the field of using ATR-FTIR spectroscopy and spectroscopic imaging to characterise and evaluate biopharmaceuticals, both in industrial and academic research based environments.

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Section: In-linementioning

confidence: 92%

mentioning

confidence: 99%

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

Tiernan

Byrne

Kazarian

2020

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

140

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“…5,6,7,8 In contrast with other detection methods, FTIR spectroscopy allows to simultaneously collect a wealth of information such as secondary structure and post-translational modifications. 9,10 Therefore, FTIR spectroscopy with attenuated internal total reflection (ATR) configuration is currently applied to study mono-and multilayers of bioorganic samples. 11 Because the evanescent wave propagates outside of the ATR element on a very short distance only, the ATR configuration allows the detection of analytes in aqueous media when they are brought in close contact with the optical element.…”

Section: Introductionmentioning

confidence: 99%

Grafting of Oligo(ethylene glycol)-Functionalized Calix[4]arene-Tetradiazonium Salts for Antifouling Germanium and Gold Surfaces

et al. 2018

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Biosensors that can determine protein concentration and structure are highly desired for biomedical applications. For the development of such biosensors, the use of Fourier transform infrared (FTIR) spectroscopy with the attenuated internal total reflection (ATR) configuration is particularly attractive, but it requires appropriate surface functionalization of the ATR optical element. Indeed, the surface has to specifically interact with a target protein in close contact with the optical element and must display antifouling properties to prevent nonspecific adsorption of other proteins. Here, we report robust monolayers of calix[4]arenes bearing oligo(ethylene glycol) (oEG) chains, which were grafted on germanium and gold surfaces via their tetradiazonium salts. The formation of monolayers of oEGylated calix[4]arenes was confirmed by AFM, IR, and contact angle measurements. The antifouling properties of these modified surfaces were studied by ATR-FTIR spectroscopy and fluorescence microscopy, and the nonspecific absorption of bovine serum albumin was found to be reduced by 85% compared to that of unmodified germanium. In other words, the organic coating by oEGylated calix[4]arenes provides remarkable antifouling properties, opening the way for the design of germanium- or gold-based biosensors.

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“…In this region, bands could be observed at 1225, 1110 and 1050 cm −1 . These vibrations were attributed primarily to C-C and C-O(H) bonds and in conjunction with the contribution of O-H bonds in the ~3370 cm −1 region, they provide an indication of the presence of carbohydrate moieties in the peptide [52,53]. Finally, it should be noted that a shoulder was observed in the amide I band at 1720 cm −1 .…”

Section: Cod Removal and The Effect Of Different Iron Formsmentioning

confidence: 87%

Iron-Stimulated Production and Antimicrobial Potential of a Novel Biosurfactant Produced by a Drilling Waste-Degrading Pseudomonas citronellolis Strain

et al. 2021

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A Pseudomonas citronellolis strain was isolated from drilling waste (DW). This strain utilizes DW as the sole energy and carbon source to produce biosurfactants (BSs). The BS produced was thermally stable, amorphous and includes a peptide structure. FeSO4, FeCl3 and Fe(NO3)3 were supplemented at various concentration levels to assess possible enhancement of BS production and DW biodegradation. The limit concentration of Fe compounds between the increase in BS formation and microbial toxicity was 0.1 mM. FeCl3 enhanced DW biodegradation and more than doubled the BS formation yield, determining an optimization strategy for BS production. The BS was then partially purified and used against several Gram-negative and positive multi-drug resistant bacteria (such as Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli spp, Acinetobacter baumaniii, Enterococcus faecalis spp, Streptococcus pneumoniae, Staphylococcus aureus, Salmonella enterica). The minimum inhibitory concentration was defined at a range of 0.25 to 10 mg/mL. The antimicrobial properties of the partially purified BS established its effectiveness and suggested a down-stream processing cost reduction, as no additional purification steps were necessary. The study could lead to a sustainable low-cost bioprocess towards a circular bioeconomy because waste, a non-expensive substrate, is used; while the BS holds great potential as a novel compound with antibiotic and disinfectant-like action, following toxicity testing with human cells.

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FTIR spectroscopy as an analytical tool to compare glycosylation in therapeutic monoclonal antibodies

Cited by 55 publications

References 23 publications

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

Grafting of Oligo(ethylene glycol)-Functionalized Calix[4]arene-Tetradiazonium Salts for Antifouling Germanium and Gold Surfaces

Iron-Stimulated Production and Antimicrobial Potential of a Novel Biosurfactant Produced by a Drilling Waste-Degrading Pseudomonas citronellolis Strain

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