FTIR and XPS studies of protein adsorption onto functionalized bioactive glass

Gruian, C.; Vanea, E.; Simon, S.; Simon, V.

doi:10.1016/j.bbapap.2012.04.008

Cited by 86 publications

(85 citation statements)

References 42 publications

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“…For tested SBF amide groups. 64,[66][67][68][70][71][72] In the case of the DCC samples, one can notice a significant increase in the intensity of this component, in good agreement with the N1s findings ( Figure 15A). Therefore, the XPS results confirmed the hypothesis of the formation of an organic screening layer (of amino acid and/or protein nature) that induced latency in the bioactivity process.…”

Section: Discussionsupporting

confidence: 78%

“…The peaks situated at lower binding energy (ie, ~398.9 eV and 399.9-400.7 eV) were associated with neutral amine and carbon-bonded nitrogen in an amidic bond, respectively. [64][65][66][67][68][69][70][71][72] The peak situated at higher binding energy (~400.3-402.2 eV) pertained to the protonated amine groups. In the case of samples tested in DS (which contained only amino acids), the spectra exhibited only the maxima of amino groups: protonated amino groups at higher binding energy (~400.3 eV), and unprotonated (neutral) amino groups at lower binding energies (~398.9 eV) ( Figure 15A).…”

Section: Discussionmentioning

confidence: 99%

“…65,68,69 When amino acids and proteins are present in the environment (DS, DC, DCC), a new component appears at ~285.9-286.1 eV, associated with C-N bonds. 64,66,67,[70][71][72] Its position is rather robust, irrespective of changes in the testing conditions (Table 3).…”

Section: Discussionmentioning

confidence: 99%

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Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

Popa¹,

Stan²,

Husanu³

et al. 2017

IJN

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Synthetic physiological fluids are currently used as a first in vitro bioactivity assessment for bone grafts. Our understanding about the interactions taking place at the fluid–implant interface has evolved remarkably during the last decade, and does not comply with the traditional International Organization for Standardization/final draft International Standard 23317 protocol in purely inorganic simulated body fluid. The advances in our knowledge point to the need of a true paradigm shift toward testing physiological fluids with enhanced biomimicry and a better understanding of the materials’ structure-dissolution behavior. This will contribute to “upgrade” our vision of entire cascades of events taking place at the implant surfaces upon immersion in the testing media or after implantation. Starting from an osteoinductive bioglass composition with the ability to alleviate the oxidative stress, thin bioglass films with different degrees of polymerization were deposited onto titanium substrates. Their biomineralization activity in simulated body fluid and in a series of new inorganic–organic media with increasing biomimicry that more closely simulated the human intercellular environment was compared. A comprehensive range of advanced characterization tools (scanning electron microscopy; grazing-incidence X-ray diffraction; Fourier-transform infrared, micro-Raman, energy-dispersive, X-ray photoelectron, and surface-enhanced laser desorption/ionization time-of-flight mass spectroscopies; and cytocompatibility assays using mesenchymal stem cells) were used. The information gathered is very useful to biologists, biophysicists, clinicians, and material scientists with special interest in teaching and research. By combining all the analyses, we propose herein a step forward toward establishing an improved unified protocol for testing the bioactivity of implant materials.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

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Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

Popa¹,

Stan²,

Husanu³

et al. 2017

IJN

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“…3,39,46 This biopolymer has regions already identified as amide I, between 1,700-1,600 cm, , due to the deformation of CH 2 and CH 3 ; amide II between 1,600-1,500 cm -1 , due to C = N stretching and N = H deformation, and amide III between 1,300-1,180 cm -1 , by the C-N stretching and N-H deformation, which are the main components in the formation of tissue repair. 3,34,35 At 7 days, the spectra were similar for all treatment groups compared with the control. However, at 14 days, the LED + D group had higher amounts of type I collagen (higher a-helix value) and more organized collagenous fibers, confirming the results of the histological analysis.…”

Section: Discussionmentioning

confidence: 67%

“…To identify the main vibrational bands contributions, the spectra were divided in seven regions and the band assignments are shown in Table 1. 3,26,[32][33][34][35][36][37][38] The spectral second derivatives were performed at an experimental time of 14 days, as depicted in Fig. 6.…”

Section: Morphometric Analysismentioning

confidence: 99%

Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions

Cronemberger

Raniero

Bueno

et al. 2016

Advances in Wound Care

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Objective: Compare the efficacy of light-emitting diode (LED) and therapeutic ultrasound (TUS), combined with a semipermeable dressing (D), at forming collagen in skin lesions by morphometry and Fourier transform infrared spectroscopy (FT-IR). Materials and Methods: Surgical skin wounds (2.5 cm) were created on 84 male Wistar rats divided into four groups (n = 21): Group I (Control), Group II (LED), Group III (LED + D), and Group IV (US + D). On days 7, 14, and 21, the tissue samples were removed and divided into two pieces, one was used for histological examination (collagen) and the other for FT-IR. Results: The histomorphometric analysis showed no significant differences among groups for collagen deposition at 7 days. However, at 14 days, more deposition of collagen was noted in the groups LED ( p < 0.05) and LED + D ( p < 0.001) than in the control. At 21 days, the groups LED, LED + D, and US + D presented significantly greater deposition of collagen when compared with the control. The FT-IR spectra, at 14 days, LED + D had greater amounts of type I collagen, a better organization of fibers, and greater difference of mean separation between the groups, not observed at 7 and 21 days. Innovation: The histomorphometric and FT-IR analyses suggest that the association of semipermeable dressing to LED therapy and to TUS modulates biological events, increasing fibroblast/collagen response and accelerating dermal maturation. Conclusion: The histomorphometric and FT-IR analyses showed that LED therapy is more efficacious than TUS, when combined with a semipermeable dressing, and induced the collagen production in skin lesions.

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