FT-IR spectral studies on certain human urinary stones in the patients of rural area

Selvaraju, R.; Thiruppathi, G.; Raja, A.

doi:10.1016/j.saa.2012.03.028

Cited by 41 publications

(24 citation statements)

References 13 publications

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“…2A. This FT-IR spectral pattern was in concordance with several lines of evidence reporting the signal stretching vibration of O-H group at 3600–2800 cm −1 , C=O group at 1615 cm −1 , C-O group at 1315 cm −1 , and O-C=O/C-C group at 780 cm −1 in the CaOx molecule 38, 39 . Fortunately, COM and COD crystals could be discriminated from each other by curve fitting analysis using FT-IR spectra between 3600–2800 cm −1 wavenumber 28, 29 .…”

Section: Discussionsupporting

confidence: 90%

Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells

Manissorn

Fong-ngern

Peerapen

et al. 2017

Sci Rep

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Urine pH has been thought to be an important factor that can modulate kidney stone formation. Nevertheless, there was no systematic evaluation of such pH effect. Our present study thus addressed effects of differential urine pH (4.0–8.0) on calcium oxalate (CaOx) crystallization, crystal-cell adhesion, crystal internalization into renal tubular cells, and binding of apical membrane proteins to the crystals. Microscopic examination revealed that CaOx monohydrate (COM), the pathogenic form, was crystallized with greatest size, number and total mass at pH 4.0 and least crystallized at pH 8.0, whereas COD was crystallized with the vice versa order. Fourier-transform infrared (FT-IR) spectroscopy confirmed such morphological study. Crystal-cell adhesion assay showed the greatest degree of crystal-cell adhesion at the most acidic pH and least at the most basic pH. Crystal internalization assay using fluorescein isothiocyanate (FITC)-labelled crystals and flow cytometry demonstrated that crystal internalization into renal tubular cells was maximal at the neutral pH (7.0). Finally, there were no significant differences in binding capacity of the crystals to apical membrane proteins at different pH. We concluded that the acidic urine pH may promote CaOx kidney stone formation, whereas the basic urine pH (i.e. by alkalinization) may help to prevent CaOx kidney stone disease.

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Section: Discussionsupporting

confidence: 90%

Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells

Manissorn

Fong-ngern

Peerapen

et al. 2017

Sci Rep

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“…All COM crystals had five stretching vibration peaks of O-H bond of crystal water at 3485-3047 cm − 1 , which was a characteristic peak of COM, whereas COD only had a single broad absorption peak in this region (Fig. 5B) [25]. The asymmetric stretching vibration (ν as ) and symmetric stretching vibration (ν s ) of carboxyl group (-COO) in COM were about at 1624 cm − 1 and 1319 cm −1 , respectively, while COD appeared at 1647 cm −1 and 1326 cm −1 [24].…”

Section: Stirring Speedmentioning

confidence: 99%

Preparation, characterization, and in vitro cytotoxicity of COM and COD crystals with various sizes

Sun

Ouyang

Liu

et al. 2015

Materials Science and Engineering: C

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“…The weak bands around at 501cm-1, 503cm-1 are due to O-C-O plane bending of undoped and manganese doped calcium oxalate monohydrate (15,16).…”

Section: Ft-raman Analysismentioning

confidence: 99%

Growth and characterization of manganese doped and undoped calcium oxalate monohydrate crystal

Selvaraju¹,

Sulochana²

2017

Int J Recent Sci Res

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Urinary stone disease is one of the major diseases in the human history. The human urinary calculi are mainly formed from calcium oxalate, Struvite and uric acid crystals. It is important for the physician and surgeon to distinguish the two types of hydrates of calcium oxalate mainly mono and di-hydrate. Manganese doped Calcium Oxalate Monohydrate (MnCaOxM) crystals were grown by silica gel method. Manganese is a trace mineral that is present in tiny amounts in the body. It is found mostly in bones and blood clotting factors. It is also plays a role in fat and carbohydrate metabolism. Manganese is also necessary for normal brain and nerve function. The grown crystals were characterized by FTIR, FT-Raman XRD, SEM-EDX, TGA/DTA analysis and UV-Visible analysis.

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FT-IR spectral studies on certain human urinary stones in the patients of rural area

Cited by 41 publications

References 13 publications

Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells

Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells

Preparation, characterization, and in vitro cytotoxicity of COM and COD crystals with various sizes

Growth and characterization of manganese doped and undoped calcium oxalate monohydrate crystal

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