FSH induced stimulation of catalase activity in goat granulosa cells in vitro

Behl, Rahul; Pandey, Rajesh

doi:10.1016/s0378-4320(02)00006-4

Cited by 39 publications

(33 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…13 Catalase activity and E 2 release were greater in large follicles than in medium or small follicles. Since the dominant follicle will be the follicle with the highest estrogen concentration, the concomitant increases in catalase and E 2 in response to FSH suggest a role for catalase in follicular selection and prevention of apoptosis.…”

Section: Resultsmentioning

confidence: 88%

Levels of prolactin, progesterone, estradiol, luteinizing hormone and follicle stimulating hormone in infertile women in Calabar, Nigeria

Isong

Okhormhe

Bassey

et al. 2016

Int J Reprod Contracept Obstet Gynecol

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 88%

Levels of prolactin, progesterone, estradiol, luteinizing hormone and follicle stimulating hormone in infertile women in Calabar, Nigeria

Isong

Okhormhe

Bassey

et al. 2016

Int J Reprod Contracept Obstet Gynecol

View full text Add to dashboard Cite

“…Little is known about the effects of GnRH or FSH on the vasculature or oxidative status. Behl and Pandey 48 described an activation of catalase by FSH in granulose cells. Although we cannot exclude that GnRH or FSH treatment affected SOD expression in circulating monocytes of these women, based on our findings in vitro and in vivo, the observed upregulation of ecSOD and MnSOD may at least partially be explained with increasing estrogen levels.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Antioxidant Enzyme Expression and Function by Estrogen

Strehlow¹,

Rotter²,

Waßmann³

et al. 2003

Circulation Research

407

259

View full text Add to dashboard Cite

Abstract-Oxidative stress plays a pivotal role in the pathogenesis of atherosclerosis and can be effectively influenced by radical scavenging enzyme activity and expression. The vasoprotective effects of estrogens may be related to antioxidative properties. Therefore, effects of 17␤-estradiol on production of reactive oxygen species and radical scavenging enzymes were investigated. 17␤-estradiol diminished angiotensin II-induced free radical production in vascular smooth muscle cells (DCF fluorescence laser microscopy). 17␤-estradiol time-and concentration-dependently upregulated manganese (MnSOD) and extracellular superoxide dismutase (ecSOD) expression (Northern and Western blotting) and enzyme activity (photometric assay). Nuclear run-on assays demonstrated that 17␤-estradiol increases MnSOD and ecSOD transcription rate. Half-life of MnSOD mRNA was not influenced, whereas ecSOD mRNA was stabilized by estrogen. Copper-zinc SOD, glutathione-peroxidase, and catalase were not affected by estrogen. Estrogen deficiency in ovariectomized mice induced a downregulation of ecSOD and MnSOD expression, which was associated with increased production of vascular free radicals and prevented by estrogen replacement or treatment with PEG-SOD. In humans, increased estrogen levels led to enhanced ecSOD and MnSOD expression in circulating monocytes. Estrogen acts antioxidative at least to some extent via stimulation of MnSOD and ecSOD expression and activity, which may contribute to its vasoprotective effects.

show abstract

“…shown that FSH stimulates catalase activity in goat granulosa cells modulating intracellular ROS levels [42]. ROS inhibitors, in a concentration-dependent manner decreased oocyte maturation induced by FSH [43].…”

Section: Discussionmentioning

confidence: 92%

Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

Mbemya¹,

Guerreiro²,

Donfack³

et al. 2017

JPP

View full text Add to dashboard Cite

Objectives: Evaluating the addition effect of J. insularis extract and FSH on the survival, activation and ROS production after in vitro culture of ovine preantral follicles enclosed in ovarian tissue. Methods: In the first experiment, ovarian fragments were fixed (non-cultured control) or in vitro cultured in α-MEM + (cultured control), α-MEM + supplemented with FSH 50 ng/mL, or in α-MEM + supplemented with J. insularis (JUS0.3; 1.25 or 5 mg/mL) for 1 or 7 days, at 39˚C, 5% CO 2 . In the second experiment, fragments were fixed or cultured in α-MEM + supplemented with anethole 300 µg/mL + FSH 50 ng/mL or in α-MEM + supplemented with anethole 300 µg/mL + 0.3 mg/mL JUS. Key findings: JUS0.3 was the only treatment that maintained the percentage of morphologically normal follicles similar to non-cultured control even after 7 days of culture. After 7 days of culture, a higher (p < 0.05) percentage of developing follicles was observed in JUS5 treatment compared with the other treatments except JUS1.25. In the second experiment, FSH maintained the percentage of normal follicles and promoted activation of primordial follicles. A reduction (p < 0.05) of stromal cell density was observed in MEM + +ANE supplemented with JUS or FSH. Conclusions: J. insularis in a concentration-dependent manner maintained the levels of ROS and improved in vitro follicular survival and activation of ovine primordial follicles.

show abstract

FSH induced stimulation of catalase activity in goat granulosa cells in vitro

Cited by 39 publications

References 21 publications

Levels of prolactin, progesterone, estradiol, luteinizing hormone and follicle stimulating hormone in infertile women in Calabar, Nigeria

Levels of prolactin, progesterone, estradiol, luteinizing hormone and follicle stimulating hormone in infertile women in Calabar, Nigeria

Modulation of Antioxidant Enzyme Expression and Function by Estrogen

Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

Contact Info

Product

Resources

About