From rotten grapes to industrial exploitation: Komagataeibacter europaeus SGP37, a micro-factory for macroscale production of bacterial nanocellulose

Dubey, Swati; Sharma, Raj Kumar; Agarwal, Pragati; Singh, Jaswant; Sinha, Neeraj; Singh, Rajesh

doi:10.1016/j.ijbiomac.2016.12.016

Cited by 62 publications

(26 citation statements)

References 34 publications

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“…It has long been recognized that the addition of ethanol significantly increases BNC yields from several strains of the Komagataeibacter genus (Naritomi et al 1998b; Krystynowicz et al 2002; Park et al 2003; Dubey et al 2017; Liu et al 2018; Molina-Ramírez et al 2018). Given the spectacular influence of ethanol on cellulose synthesis, efforts have been made to better understand its molecular basis.…”

Section: Discussionmentioning

confidence: 99%

“…Alternative substrates, such as ethanol, acetic acid, lactic acid, and sodium citrate have been tested for their ability to promote cellulose production (Matsuoka et al 1996; Naritomi et al 1998a, b; Li et al 2012; Molina-Ramírez et al 2018). Ethanol has been shown to have a particularly strong inducing effect on BNC synthesis in the case of the Komagataeibacter species (Naritomi et al 1998b; Krystynowicz et al 2002; Park et al 2003; Dubey et al 2017; Liu et al 2018; Molina-Ramírez et al 2018). Ethanol is naturally present in the environments from which many Komagataeibacter species are isolated, such as vinegar or the fermented tea beverage, Kombucha.…”

Section: Introductionmentioning

confidence: 99%

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Effect of ethanol supplementation on the transcriptional landscape of bionanocellulose producer Komagataeibacter xylinus E25

Ryngajłło

Jacek

Cielecka

et al. 2019

Appl Microbiol Biotechnol

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Ethanol exerts a strong positive effect on the cellulose yields from the widely exploited microbial producers of the Komagataeibacter genus. Ethanol is postulated to provide an alternative energy source, enabling effective use of glucose for cellulose biosynthesis rather than for energy acquisition. In this paper, we investigate the effect of ethanol supplementation on the global gene expression profile of Komagataeibacter xylinus E25 using RNA sequencing technology (RNA-seq). We demonstrate that when ethanol is present in the culture medium, glucose metabolism is directed towards cellulose production due to the induction of genes related to UDP-glucose formation and the repression of genes involved in glycolysis and acetan biosynthesis. Transcriptional changes in the pathways of cellulose biosynthesis and c-di-GMP metabolism are also described. The transcript level profiles suggest that Schramm-Hestrin medium supplemented with ethanol promotes bacterial growth by inducing protein biosynthesis and iron uptake. We observed downregulation of genes encoding transposases of the IS 110 family which may provide one line of evidence explaining the positive effect of ethanol supplementation on the genotypic stability of K. xylinus E25. The results of this study increase knowledge and understanding of the regulatory effects imposed by ethanol on cellulose biosynthesis, providing new opportunities for directed strain improvement, scaled-up bionanocellulose production, and wider industrial exploitation of the Komagataeibacter species as bacterial cellulose producers. Electronic supplementary material The online version of this article (10.1007/s00253-019-09904-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of ethanol supplementation on the transcriptional landscape of bionanocellulose producer Komagataeibacter xylinus E25

Ryngajłło

Jacek

Cielecka

et al. 2019

Appl Microbiol Biotechnol

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“…The most widely used species of Gluconacetobacter is Gluconacetobacter xylinus (synonyms Acetobacter xylinus , Komagataeibacter xylinus ) [1,49]. Other important species include Gluconacetobacter hansenii [3,50,51], Gluconacetobacter kombuchae [52], Komagataeibacter (Gluconacetobacter) europaeus [53], and low pH-resistant strain Komagataeibacter (Gluconacetobacter) medellinensis [42]. The bacterial growth and production of nanocellulose can be further enhanced by the presence of yeasts or yeast extract in the culture medium [52,54], or by symbiotic co-cultivation with Мedusomyces gisevii [55].…”

Section: Introductionmentioning

confidence: 99%

Versatile Application of Nanocellulose: From Industry to Skin Tissue Engineering and Wound Healing

et al. 2019

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Nanocellulose is cellulose in the form of nanostructures, i.e., features not exceeding 100 nm at least in one dimension. These nanostructures include nanofibrils, found in bacterial cellulose; nanofibers, present particularly in electrospun matrices; and nanowhiskers, nanocrystals, nanorods, and nanoballs. These structures can be further assembled into bigger two-dimensional (2D) and three-dimensional (3D) nano-, micro-, and macro-structures, such as nanoplatelets, membranes, films, microparticles, and porous macroscopic matrices. There are four main sources of nanocellulose: bacteria (Gluconacetobacter), plants (trees, shrubs, herbs), algae (Cladophora), and animals (Tunicata). Nanocellulose has emerged for a wide range of industrial, technology, and biomedical applications, namely for adsorption, ultrafiltration, packaging, conservation of historical artifacts, thermal insulation and fire retardation, energy extraction and storage, acoustics, sensorics, controlled drug delivery, and particularly for tissue engineering. Nanocellulose is promising for use in scaffolds for engineering of blood vessels, neural tissue, bone, cartilage, liver, adipose tissue, urethra and dura mater, for repairing connective tissue and congenital heart defects, and for constructing contact lenses and protective barriers. This review is focused on applications of nanocellulose in skin tissue engineering and wound healing as a scaffold for cell growth, for delivering cells into wounds, and as a material for advanced wound dressings coupled with drug delivery, transparency and sensorics. Potential cytotoxicity and immunogenicity of nanocellulose are also discussed.

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“…Nanocellulose fibers have attracted significant interest in the scientific community over the past 20 years due to outstanding mechanical properties [28,29], high specific surface area and interesting optical characteristics [30]. Great attention was paid to the isolation of nanofibers from cellulosic material and different methods are known to obtain this type of fibers such as acid hydrolysis [31], mechanical treatment [32][33][34] and bacterial nanocellulose [35]. Different kinds of surface functionalization were adopted to improve the adhesion between the hydrophilic surface of the cellulose and the hydrophobic behavior of PLA to improve the adhesion properties.…”

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confidence: 99%

Polylactic acid-lauryl functionalized nanocellulose nanocomposites: Microstructural, thermo-mechanical and gas transport properties

Rigotti¹,

Checchetto²,

Tarter³

et al. 2019

Express Polym. Lett.

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According to the European Bioplastics association, in 2017, the so-called bioplastics represented only 2.06 million tons of the 320 million tons of plastics produced annually [1]. The global production capacity is expected to increase up to 2.62 million tons in 2023. This growth is pushed by the demand of more sophisticated biopolymers, new applications and products. From packaging, agriculture, consumer electronics, textile to automotive, bioplastics are used in an increasing number of applications. In fact, biopolymers offer a number of additional advantages if compared to conventional plastics, such as a reduced carbon footprint and additional waste management options. Among bio-based and biodegradable plastics, polylactic acid (PLA) is one of the most interesting ones due to their good mechanical properties, good workability, excellent barrier properties. Therefore, it is a good candidate for the replacement of polystyrene (PS), polypropylene (PP), and acrylonitrile butadiene styrene (ABS) in many applications. However, PLA presents some weak points mainly represented by its low ductility, poor toughness, low glass transition temperature, high sensitivity to moisture and relatively low gas barrier, that limit its use in packaging applications [2, 3]. Indeed, the main

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From rotten grapes to industrial exploitation: Komagataeibacter europaeus SGP37, a micro-factory for macroscale production of bacterial nanocellulose

Cited by 62 publications

References 34 publications

Effect of ethanol supplementation on the transcriptional landscape of bionanocellulose producer Komagataeibacter xylinus E25

Effect of ethanol supplementation on the transcriptional landscape of bionanocellulose producer Komagataeibacter xylinus E25

Versatile Application of Nanocellulose: From Industry to Skin Tissue Engineering and Wound Healing

Polylactic acid-lauryl functionalized nanocellulose nanocomposites: Microstructural, thermo-mechanical and gas transport properties

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