From microbial genomics to meta-genomics

Counterselectable Markers: Untapped Tools for Bacterial Genetics and Pathogenesis

“…However, to date a great number of open reading frames (ORFs) remain unannotated since they present no homology with sequences already present in the databases (9,13). The precise function of some of these unknown genes will probably be deduced through in silico predictions, by comparing different genomes, or by the use of modern genetic strategies such as serial analysis of gene expression, in vivo expression technology, representational difference analysis, and signature tagged mutagenesis that help in extraction of information without a priori knowledge of the sequence (9,17). Nevertheless, reverse genetic analysis, one of the logical approaches to be undertaken, will be necessary to identify a phenotype and to attribute a precise function to many undefined ORFs.…”

Structure‐activity relationship approaches and applications

“…By enabling the rapid synthesis and biological evaluation of new chemical entities, combinatorial chemistry and HTS have led to the creation of vast libraries of ''drug like'' chemicals and associated biological data [1,2]. Microarray and protein array systems are just as rapidly discovering genes and proteins, many of which will serve as the key targets for drugs that treat unmet medical needs [3][4][5][6][7][8][9][10].…”

A Web-Based Research Tool for Functional Genomics of the Microcirculation: The Leukocyte Adhesion Cascade

“…Complete genomic information has recently become available for several prokaryotes (9), the yeast saccharomyces cerevisiae (23), and the nematode caenorhabditis elegans (7,28). The first mammalian genome to be completed is likely to be the human genome, which is predicted to be available within 2-3 years.…”