From mesoderm to blood islands: patterns of key molecules during yolk sac erythropoiesis

Minkó, Krisztina; Bollérot, Karine; Drevon, C.; Hallais, Marie; Jaffredo, Thierry

doi:10.1016/s1567-133x(03)00053-x

Cited by 49 publications

(46 citation statements)

References 52 publications

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“…1A, 2A; arrows). The extraembryonic mesoderm at this stage strongly expresses hemangioblast, hematopoietic and endothelial precursor markers such as Gata2, Scl, Lmo2, Ets1 and Vegfr2 Minko et al, 2003;Nakazawa et al, 2006;Shin et al, 2009), indicating that Endoglin expression does correlate with the initial steps of endothelial precursor specification.…”

Section: Resultsmentioning

confidence: 92%

Dynamic expression of Endoglin, a TGF-beta co-receptor, during pre-circulation vascular development in chick

Alev¹,

McIntyre²,

Ota³

et al. 2010

Int. J. Dev. Biol.

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Section: Resultsmentioning

confidence: 92%

Dynamic expression of Endoglin, a TGF-beta co-receptor, during pre-circulation vascular development in chick

Alev¹,

McIntyre²,

Ota³

et al. 2010

Int. J. Dev. Biol.

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“…The discovery of Gata2 and characterization of its expression pattern (Minko et al, 2003), and the knockout phenotype of Gata2 -/-animals (Tsai et al, 1994), implicated Gata2 as an ideal candidate in molecular control of hemangioblast development and differentiation. A number of reports have implicated GATA factors as being downstream of BMP4 signaling, including Gata2 (Maeno et al, 1996;Friedle and Knochel, 2002) and Gata4 (Rojas et al, 2005).…”

Section: Gata2 and Bmp4 In Mesoderm Differentiationmentioning

confidence: 99%

GATA2 functions at multiple steps in hemangioblast development and differentiation

et al. 2007

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*Molecular mechanisms that regulate the generation of hematopoietic and endothelial cells from mesoderm are poorly understood. To define the underlying mechanisms, we compared gene expression profiles between embryonic stem (ES) cell-derived hemangioblasts (Blast-Colony-Forming Cells, BL-CFCs) and their differentiated progeny, Blast cells. Bioinformatic analysis indicated that BL-CFCs resembled other stem cell populations. A role for Gata2, one of the BL-CFC-enriched transcripts, was further characterized by utilizing the in vitro model of ES cell differentiation. Our studies revealed that Gata2 was a direct target of BMP4 and that enforced GATA2 expression upregulated Bmp4, Flk1 and Scl. Conditional GATA2 induction resulted in a temporal-sensitive increase in hemangioblast generation, precocious commitment to erythroid fate, and increased endothelial cell generation. GATA2 additionally conferred a proliferative signal to primitive erythroid progenitors. Collectively, we provide compelling evidence that GATA2 plays specific, contextual roles in the generation of Flk-1 + mesoderm, the Flk-1 + Scl + hemangioblast, primitive erythroid and endothelial cells.

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“…These gene expressions were preferentially found in cells located in the ventral aspect of the mesoderm and were accompanied by the appearance of morphologically distinct structures. At later stages, these structures became more conspicuous accompanied by the expression of SCL/Tal1, Lmo2 and the appearance of GATA-1 (Minko et al, 2003). Gene activities have been also thoroughly characterized at the level of the aorta.…”

Section: Molecular Characterisation Of the Hemangioblast And The Hemomentioning

confidence: 99%

Tracing the hemangioblast during embryogenesis: developmental relationships between endothelial and hematopoietic cells

Jaffredo¹,

Bollérot²,

Sugiyama³

et al. 2005

Int. J. Dev. Biol.

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We review here the development of the hematopoietic system and its relationship to the endothelium, with a special focus on the characterisation of the hemangioblast, the putative ancestor for endothelial cells and hematopoietic cells. Using the avian model, we have traced in vivo the progeny of embryonic endothelial cells and shown that aortic-born hematopoietic cells (known to generate the definitive hematopoietic lineage) derive from endothelial cells in the floor of the aorta. During this process, endothelial cells undergo a switch from endothelial cells to hematopoietic cells characterised by a downgrading of endothelial cell-specific genes and the parallel upgrading of hematopoietic cell-specific genes. Using a similar approach, we have shown that generation of hematopoietic cells from endothelial cells also takes place during mouse embryonic development. We have thoroughly characterised the dynamics of key molecules (several of which we have cloned) specifically expressed by the yolk sac or aortic hemangioblast. The yolk sac hemangioblast is characterized by the specific expression of SCL/Tal-1 and Lmo2, whereas the aortic hemangioblast expresses Runx-1 (a runt domain transcription factor). Finally, we have demonstrated the existence of a new site for hematopoiesis, namely the allantois. Using quail/chick grafts, we show that this embryonic appendage autonomously produces endothelial cells and hematopoietic cells, these latter being endowed with the attributes of the definitive hematopoietic lineage.

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From mesoderm to blood islands: patterns of key molecules during yolk sac erythropoiesis

Cited by 49 publications

References 52 publications

Dynamic expression of Endoglin, a TGF-beta co-receptor, during pre-circulation vascular development in chick

Dynamic expression of Endoglin, a TGF-beta co-receptor, during pre-circulation vascular development in chick

GATA2 functions at multiple steps in hemangioblast development and differentiation

Tracing the hemangioblast during embryogenesis: developmental relationships between endothelial and hematopoietic cells

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