From folklore to pharmacy: Putting plants into practice

Graham‐Brown, R.A.C.; Healsmith, Mark F.

doi:10.1016/j.clindermatol.2018.03.002

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…Medicinal plants are low in costs and show strong pharmacological potential, exhibit anti-oxidant, anti-inflammatory, or anti-proliferative potential, and are therefore increasingly tested against various morbidities [ 13 ]. Medicinal plants have been used for millennia, but only over the last few decades did molecular approaches of drug discovery allow for identification of active fractions and components, and toxicological as well as pharmacodynamical studies have been performed [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Maca against Echinococcosis?—A Reverse Approach from Patient to In Vitro Testing

et al. 2021

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Drug-based treatment of alveolar echinococcosis (AE) with benzimidazoles is in most cases non-curative, thus has to be taken lifelong. Here, we report on a 56-year-old male AE patient who received standard benzimidazole treatment and biliary plastic stents, and additionally self-medicated himself with the Peruvian plant extract Maca (Lepidium meyenii). After 42 months, viable parasite tissue had disappeared. Based on this striking observation, the anti-echinococcal activity of Maca was investigated in vitro and in mice experimentally infected with Echinococcus multilocularis metacestodes. Albendazole (ABZ)-treated mice and mice treated with an ABZ+Maca combination exhibited a significantly reduced parasite burden compared to untreated or Maca-treated mice. As shown by a newly established UHPLC-MS/MS-based measurement of ABZ-metabolites, the presence of Maca during the treatment did not alter ABZ plasma levels. In vitro assays corroborated these findings, as exposure to Maca had no notable effect on E. multilocularis metacestodes, and in cultures of germinal layer cells, possibly unspecific, cytotoxic effects of Maca were observed. However, in the combined treatments, Maca inhibited the activity of ABZ in vitro. While Maca had no direct anti-parasitic activity, it induced in vitro proliferation of murine spleen cells, suggesting that immunomodulatory properties could have contributed to the curative effect seen in the patient.

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Section: Introductionmentioning

confidence: 99%

Maca against Echinococcosis?—A Reverse Approach from Patient to In Vitro Testing

et al. 2021

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“…Medicinal plants have been in use since ever for the treatment of human diseases, and over the past decades they have attracted increasing interest in the search for treatments against infectious agents. They exhibit powerful pharmacological activities, they are accessible, are relatively low in cost, and have generally low toxicities (Graham-Brown and Healsmith, 2018; Rogozea, 2018). The pharmaceutical properties of aromatic plants are partially attributed to essential oils (EO) (Maggiore et al ., 2012).…”

Section: Introductionmentioning

confidence: 99%

Activity of Thymus capitatus essential oil components against in vitro cultured Echinococcus multilocularis metacestodes and germinal layer cells

et al. 2019

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The essential oil (EO) of Thymus capitatus, seven fractions (F1–F7) obtained from silica gel chromatography, and several pure EO components were evaluated with respect to in vitro activities against Echinococcus multilocularis metacestodes and germinal layer (GL) cells. Attempts to evaluate physical damage in metacestodes by phosphoglucose isomerase (PGI) assay failed because EO and F1–F7 interfered with the PGI-activity measurements. A metacestode viability assay based on Alamar Blue, as well as transmission electron microscopy, demonstrated that exposure to EO, F2 and F4 impaired metacestode viability. F2 and F4 exhibited higher toxicity against metacestodes than against mammalian cells, whereas EO was as toxic to mammalian cells as to the parasite. However, none of these fractions exhibited notable activity against isolated E. multilocularis GL cells. Analysis by gas chromatography-mass spectrometry showed that carvacrol was the major component of the EO (82.4%), as well as of the fractions F3 (94.4%), F4 (98.1%) and F5 (90.7%). Other major components of EO were β-caryophyllene, limonene, thymol and eugenol. However, exposure of metacestodes to these components was ineffective. Thus, fractions F2 and F4 of T. capitatus EO contain potent anti-echinococcal compounds, but the activities of these two fractions are most likely based on synergistic effects between several major and minor constituents.

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Commentary: Complementary dermatology

Jakus

Siegel

2018

Clinics in Dermatology

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From folklore to pharmacy: Putting plants into practice

Cited by 3 publications

References 31 publications

Maca against Echinococcosis?—A Reverse Approach from Patient to In Vitro Testing

Maca against Echinococcosis?—A Reverse Approach from Patient to In Vitro Testing

Activity of Thymus capitatus essential oil components against in vitro cultured Echinococcus multilocularis metacestodes and germinal layer cells

Commentary: Complementary dermatology

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