From Acetate to Bio-Based Products: Underexploited Potential for Industrial Biotechnology

Kiefer, Dirk; Merkel, Manuel; Lilge, Lars; Henkel, Marius; Hausmann, Rudolf

doi:10.1016/j.tibtech.2020.09.004

Cited by 101 publications

(68 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Through strain development carbon fluxes of V. natriegens were successfully re-directed towards small molecule production using mainly glucose as substrate. Since the future bioeconomy relies on the circular use of biogenic resources and waste streams such as lignocellulosic hydrolysates, biorefinery side streams and non-sugar carbon sources such as organic acids [1,[45][46][47], the (parallel) consumption of such substrates by V. natriegens, potentially impacted by catabolite repression, has to be investigated and might be optimized by substrate utilization engineering [48]. For such applications the tolerance of V. natriegens towards alternative carbon sources, (lignocellulosic) inhibitors and the envisioned products has to be analyzed and might be increased to levels of industrial relevance by methods such as adaptive laboratory evolution [49].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, most biotechnological production processes are carbohydrate-based (e.g. glucose or sucrose) [1] applying industrially established microbial systems such as Escherichia coli or Corynebacterium glutamicum. Although sugar-based fermentation approaches are generally state of the art, various developed processes do not reach commercialization level since the overall costs cannot compete with existing chemical approaches.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic engineering of Vibrio natriegens

Thoma

Blombach

2021

Essays in Biochemistry

View full text Add to dashboard Cite

Vibrio natriegens is emerging as a promising host for biotechnology which is basically due to the remarkable intrinsic properties such as the exceptionally high growth and substrate consumption rates. The facultatively anaerobic marine bacterium possesses a versatile metabolism, is able to utilize a variety of substrates as carbon and energy sources and is easy to handle in the lab. These features initiated the rapid development of genetic tools and resulted in extensive engineering of production strains in the past years. Although recent examples illustrate the potential of V. natriegens for biotechnology, a comprehensive understanding of the metabolism and its regulation is still lacking but essential to exploit the full potential of this bacterium. In this review, we summarize the current knowledge on the physiological traits and the genomic organization, provide an overview of the available genetic engineering tools and recent advances in metabolic engineering of V. natriegens. Finally, we discuss the obstacles which have to be overcome in order to establish V. natriegens as industrial production host.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of Vibrio natriegens

Thoma

Blombach

2021

Essays in Biochemistry

View full text Add to dashboard Cite

show abstract

“…However, the segmented approach (CO 2 to acetate, acetate to PHB) prevents incompatibilities that arise from combining electrochemistry and biological catalysts such as the requirement to maintain neutral pH. Additionally, a segmented process flow allows for individual unit optimization and overall biomanufacturing modularity ( Fast and Papoutsakis, 2012 ; Kiefer et al, 2021 ). For these reasons, we have designed our bioprocess to include a base medium used for both bioreactions–acetate and PHB generation.…”

Section: Introductionmentioning

confidence: 99%

Production of PHB From CO2-Derived Acetate With Minimal Processing Assessed for Space Biomanufacturing

Cestellos-Blanco¹,

Friedline²,

Sander³

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Providing life-support materials to crewed space exploration missions is pivotal for mission success. However, as missions become more distant and extensive, obtaining these materials from in situ resource utilization is paramount. The combination of microorganisms with electrochemical technologies offers a platform for the production of critical chemicals and materials from CO2 and H2O, two compounds accessible on a target destination like Mars. One such potential commodity is poly(3-hydroxybutyrate) (PHB), a common biopolyester targeted for additive manufacturing of durable goods. Here, we present an integrated two-module process for the production of PHB from CO2. An autotrophic Sporomusa ovata (S. ovata) process converts CO2 to acetate which is then directly used as the primary carbon source for aerobic PHB production by Cupriavidus basilensis (C. basilensis). The S. ovata uses H2 as a reducing equivalent to be generated through electrocatalytic solar-driven H2O reduction. Conserving and recycling media components is critical, therefore we have designed and optimized our process to require no purification or filtering of the cell culture media between microbial production steps which could result in up to 98% weight savings. By inspecting cell population dynamics during culturing we determined that C. basilensis suitably proliferates in the presence of inactive S. ovata. During the bioprocess 10.4 mmol acetate L –1 day–1 were generated from CO2 by S. ovata in the optimized media. Subsequently, 12.54 mg PHB L–1 hour–1 were produced by C. basilensis in the unprocessed media with an overall carbon yield of 11.06% from acetate. In order to illustrate a pathway to increase overall productivity and enable scaling of our bench-top process, we developed a model indicating key process parameters to optimize.

show abstract

“…A biological equivalent to a liquid intermediate-substrate that can serve as unified feedstock for various heterotrophic microbial cell factories is acetate. As alternative to methanol carbonylation 3 , numerous bioprocesses have emerged that sequestrate CO 2 into acetate and it is considered a next-generation biotech-feedstock (Kiefer et al, 2021). Processes with chemoorganoheterotrophic organisms using soluble substrates can achieve high productivities and allow for streamlined process design.…”

Section: From Autotrophy To Heterotrophy-impact On Process Parameters and Complexitymentioning

confidence: 99%

Choice of Microbial System for In-Situ Resource Utilization on Mars

Averesch

2021

Front. Astron. Space Sci.

View full text Add to dashboard Cite

Various microbial systems have been explored for their applicability to in-situ resource utilisation (ISRU) on Mars and suitability to leverage Martian resources and convert them into useful chemical products. Considering only fully bio-based solutions, two approaches can be distinguished, which comes down to the form of carbon that is being utilized: (a) the deployment of specialised species that can directly convert inorganic carbon (atmospheric CO2) into a target compound or (b) a two-step process that relies on independent fixation of carbon and the subsequent conversion of biomass and/or complex substrates into a target compound. Due to the great variety of microbial metabolism, especially in conjunction with chemical support-processes, a definite classification is often difficult. This can be expanded to the forms of nitrogen and energy that are available as input for a biomanufacturing platform. To provide a perspective on microbial cell factories that may be suitable for Space Systems Bioengineering, a high-level comparison of different approaches is conducted, specifically regarding advantages that may help to extend an early human foothold on the red planet.

show abstract

From Acetate to Bio-Based Products: Underexploited Potential for Industrial Biotechnology

Cited by 101 publications

References 104 publications

Metabolic engineering of Vibrio natriegens

Metabolic engineering of Vibrio natriegens

Production of PHB From CO2-Derived Acetate With Minimal Processing Assessed for Space Biomanufacturing

Choice of Microbial System for In-Situ Resource Utilization on Mars

Contact Info

Product

Resources

About