Metabolic engineering of Vibrio natriegens

Abstract

Vibrio natriegens is emerging as a promising host for biotechnology which is basically due to the remarkable intrinsic properties such as the exceptionally high growth and substrate consumption rates. The facultatively anaerobic marine bacterium possesses a versatile metabolism, is able to utilize a variety of substrates as carbon and energy sources and is easy to handle in the lab. These features initiated the rapid development of genetic tools and resulted in extensive engineering of production strains in the past years. Although recent examples illustrate the potential of V. natriegens for biotechnology, a comprehensive understanding of the metabolism and its regulation is still lacking but essential to exploit the full potential of this bacterium. In this review, we summarize the current knowledge on the physiological traits and the genomic organization, provide an overview of the available genetic engineering tools and recent advances in metabolic engineering of V. natriegens. Finally, we discuss the obstacles which have to be overcome in order to establish V. natriegens as industrial production host.

Genetic Code Expansion in the Engineered Organism Vmax X2: High Yield and Exceptional Fidelity

“… 77 , 78 Another potential factor is degradation of the ncAA due to cellular metabolic processes, which may differ between E. coli and V. natriegens . 79 , 80 Although ncAA side chains can also degrade during MS analysis or sample workup, as all samples were prepared identically, we would expect that such occurrences would be fairly constant. Nevertheless, if the goal is a homogeneous and uniquely modified ncAA-containing protein in high yield and purity, Vmax X2 outperformed other strains, even those that funnel GCE through a single UAG channel.…”

Choice of Microbial System for In-Situ Resource Utilization on Mars

“…It also has a rapid metabolism and growth rate, exceeded by few other organisms. Vibrio natrigens is an emerging workhorse of white biotechnology, able to use a wide range of common organic molecules as its sole source of carbon and energy (Austin et al, 1978); its biggest advantage, however, being a doubling-time just under 10 min (Thoma and Blombach, 2021). It's exceptionally fast metabolism alone may warrant employment for specialty applications in Space Synthetic Biology, potentially in combination with adaptive evolution, which strongly depends on rate of replication (Dragosits and Mattanovich, 2013).…”

Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens

“…Vibrio natriegens is an emerging non-pathogenic bacterial chassis with appealing characteristics for synthetic biology and metabolic engineering applications ( Hoff et al, 2020 ; Blombach et al, 2021 ; Thoma and Blombach, 2021 ). The characteristics include a short doubling time of less than 10 min ( Eagon, 1962 ); ability to produce small molecules ( Hoffart et al, 2017 ) and recombinant proteins in high titer ( Weinstock et al, 2016 ; Schleicher et al, 2018 ); tolerance to high salt concentrations and pH range ( Payne et al, 1961 ); and ability to grow in minimal media containing different carbon sources ( Hoffart et al, 2017 ).…”