Frizzled 2 is a key component in the regulation of TOR signaling-mediated egg production in the mosquito Aedes aegypti

Weng, Shih-Che; Shiao, Shin-Hong

doi:10.1016/j.ibmb.2015.03.010

Cited by 22 publications

(22 citation statements)

References 23 publications

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“…Here, we find that Wnt and Fz2 mRNA levels are up-regulated in OV_FC (Fig 4A and S2 Table). Additionally, Wnt and ToR signaling interact synergistically in the vitellogenic process [37] and supporting this finding, we showed mToR signaling is active in OV_FC [17]. Also, the non-canonical Wnt pathway indicates that Wnt/Fz signaling leads to the release of intracellular calcium through trimeric G proteins [37].…”

Section: Protein and Hormone Analysissupporting

confidence: 72%

“…The Wnt (glycoprotein ligand) and Frizzled (Fz, transmembrane Wnt receptor) proteins interact with structural components at the cell surface to initiate the signaling cascades that result in transcriptional regulation of gene expression. In A. aegypti, a fundamental role of Frizzled 2 (Fz2) was reported in egg production [37]. Here, we find that Wnt and Fz2 mRNA levels are up-regulated in OV_FC (Fig 4A and S2 Table).…”

Section: Protein and Hormone Analysissupporting

confidence: 56%

“…Additionally, Wnt and ToR signaling interact synergistically in the vitellogenic process [37] and supporting this finding, we showed mToR signaling is active in OV_FC [17]. Also, the non-canonical Wnt pathway indicates that Wnt/Fz signaling leads to the release of intracellular calcium through trimeric G proteins [37]. The calcium release and intracellular accumulation activates several Ca 2+ -sensitive proteins, including protein kinase C (PKC), calcineurin and calcium/calmodulin-dependent kinase II (CamKII).…”

Section: Protein and Hormone Analysismentioning

confidence: 99%

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What happens after a blood meal? A transcriptome response from the main tissues involved in egg production inRhodnius prolixus, an insect vector of Chagas disease

Leyria

Orchard

Lange

2020

Preprint

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The blood-sucking hemipteran Rhodnius prolixus is a vector of Chagas disease, one of the most neglected tropical diseases affecting several million people, mostly in Latin America.The blood meal is an event with a high epidemiological impact since in adult mated females it initiates the production of hundreds of eggs. By means of RNA-Sequencing (RNA-Seq) we have examined how a blood meal influences mRNA expression in the central nervous system (CNS), fat body and ovaries in order to promote egg production, focusing on tissue-specific responses under controlled nutritional conditions. We illustrate the cross talk between reproduction and a) lipids, proteins and trehalose metabolism, b) neuropeptide and neurohormonal signaling, and c) the immune system. Overall, our molecular evaluation confirms and supports previous studies and provides an invaluable molecular resource for future investigations on different tissues involved in successful reproductive events. Analyses like this can be used to increase the chances of developing novel strategies of vector population control by translational research, with less impact on the environment and more specificity for a particular organism.

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Section: Protein and Hormone Analysissupporting

confidence: 72%

Section: Protein and Hormone Analysissupporting

confidence: 56%

Section: Protein and Hormone Analysismentioning

confidence: 99%

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What happens after a blood meal? A transcriptome response from the main tissues involved in egg production inRhodnius prolixus, an insect vector of Chagas disease

Leyria

Orchard

Lange

2020

Preprint

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“…The high AalVg expression observed in Aalbdsx-knockdown blood-fed mosquitoes was obviously contrary to a previous report that DSX is a transcriptional activator of Vg in insects (19,31,61). Hemolymph amino acid levels regulate TOR and the transmembrane receptor Frizzled 2 (Fz2), inducing the target gene phosphorylation of S6 kinase (S6K) in the fat body (73,74). Then, upregulated S6K activates the translation level of the GATA factor, which is a key regulatory factor in Vg gene transcription (75,76).…”

Section: High Expression Of Aalvg Mainly Originates From Feedback Regmentioning

confidence: 99%

The direct regulation ofAalbdsxonAalVgRis indispensable for ovarian development inAedes albopictus

Jin

Zhao

Liu

et al. 2020

Preprint

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BACKGROUNDAedes albopictus is an important vector with an extensive worldwide distribution. Only female mosquitoes play a significant role in the transmission of pathogens. Doublesex (dsx) is a central nexus gene in the insect somatic sex determination hierarchy.RESULTSIn this study, we characterized the full-length sex-specific splicing forms of the Ae. albopictus dsx gene (Aalbdsx). Then, we identified 15 direct target genes of DSX in adult females using digital gene expression (DGE) combined with qPCR following a chromatin immunoprecipitation assay (ChIP) with specific DSX antibodies. The knockdown of Aalbdsx suppressed ovarian development, and the transcript levels of the Aalbdsx target vitellogenin receptor (VgR) gene decreased, whereas vitellogenin (Vg) expression showed an increase in the fat body. Genes in the major Vg regulatory pathway were also upregulated. Our results suggest that the effects of Aalbdsx RNAi on ovarian development are exerted mainly via VgR rather than Vg.CONCLUSIONThe results of our study not only provide a reference for the further elucidation of the sex determination cascade and comparative analyses of dsx target interactions in mosquitoes but also reveal potential molecular targets for application to the development of sterile male mosquitoes to be released for vector control.

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“…A recent study has shown a possible crosstalk between wingless (Wnt) and TOR signaling pathways in A. aegypti vitellogenesis [26]. Depletion of Frizzled 2, the transmembrane Wnt receptor that is predominantly expressed in the mosquito fat body after a blood meal, causes a significant reduction in S6K phosphorylation and subsequently in Vg expression [27].…”

Section: The Amino Acid/tor Pathway As a Nutritional Sensormentioning

confidence: 99%

Nutritional control of insect reproduction

Smýkal

Raikhel

2015

Current Opinion in Insect Science

100

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The amino acid-Target of Rapamycin (AA/TOR) and insulin pathways play a pivotal role in reproduction of female insects, serving as regulatory checkpoints that guarantee the sufficiency of nutrients for developing eggs. Being evolutionary older, the AA/TOR pathway functions as an initial nutritional sensor that not only activates nutritional responses in a tissue-specific manner, but is also involved in the control of insect insulin-like peptides (ILPs) secretion. Insulin and AA/TOR pathways also assert their nutritionally linked influence on reproductive events by contributing to the control of biosynthesis and secretion of juvenile hormone and ecdysone. This review covers the present status of our understanding of the contributions of AA/TOR and insulin pathways in insect reproduction.

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Frizzled 2 is a key component in the regulation of TOR signaling-mediated egg production in the mosquito Aedes aegypti

Cited by 22 publications

References 23 publications

What happens after a blood meal? A transcriptome response from the main tissues involved in egg production inRhodnius prolixus, an insect vector of Chagas disease

What happens after a blood meal? A transcriptome response from the main tissues involved in egg production inRhodnius prolixus, an insect vector of Chagas disease

The direct regulation ofAalbdsxonAalVgRis indispensable for ovarian development inAedes albopictus

Nutritional control of insect reproduction

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