Nutritional control of insect reproduction

Smýkal, Vlastimil; Raikhel, Alexander S.

doi:10.1016/j.cois.2015.08.003

Cited by 107 publications

(96 citation statements)

References 64 publications

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“…In female mosquitoes, a blood meal triggers a chain of regulatory signals that results in activation of mRNA expression and synthesis of YPPs in the FB. The amino acid (aa)-induced target of rapamycin (TOR) signaling pathway and 20-hydroxyecdysone (20E) are the main factors controlling YPP gene expression (5,6,27). The vitellogenin (Vg) gene, widely taken as an indicator of FB vitellogenic status, reaches its maximal expression at 24 h PBM and declines dramatically thereafter at around 36-40 h PBM (28).…”

Section: Significancementioning

confidence: 99%

Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle of Aedes aegypti

Zhang

Aksoy

Girke

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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The mosquito Aedes aegypti is a major vector of numerous viral diseases, because it requires a blood meal to facilitate egg development. The fat body, a counterpart of mammalian liver and adipose tissues, is the metabolic center, playing a key role in reproduction. Therefore, understanding of regulatory networks controlling its functions is critical, and the role of microRNAs (miRNAs) in the process is largely unknown. We aimed to explore miRNA expression and potential targets in the female fat body of Ae. aegypti, as well as their changes postblood meal (PBM). Small RNA library analysis revealed five unique miRNA patterns sequentially expressed at five sampled time points, likely responding to, and affecting, waves of upstream hormonal signals and gene expression in the same period. To link miRNA identities with downstream targets, transcriptome-wide mRNA 3′ UTR interaction sites were experimentally determined at 72 h posteclosion and 24 h PBM through Argonaute 1 cross-linking and immunoprecipitation followed by high-throughput sequencing. Several target sites were validated by means of in vitro luciferase assays with wild-type and mutated 3′ UTRs for six miRNA families. With established transgenic lines, consistent results were observed with spatiotemporal knockdown of miR-8 and luciferase assays. We further investigated miRNAs potentially regulating various physiological processes based on Clusters of Orthologous Groups functional categories. Hence, the present work comprehensively elucidated miRNA expression and target dynamics in the female mosquito fat body, providing a solid foundation for future functional studies of miRNA regulation during the gonadotrophic cycle.Aedes aegypti | fat body | microRNA | microRNA targets | Argonaute 1

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Section: Significancementioning

confidence: 99%

Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle of Aedes aegypti

Zhang

Aksoy

Girke

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…(A) Mosquitoes were fed with: (1) 10% sugar, (2) blood or (3) P. falciparum -infected blood. At 18 h post blood feeding, mosquitoes from each group were dissected.…”

Section: Resultsmentioning

confidence: 99%

“…The gonotrophic cycle is divided into two phases – the pre-vitellogenic (PV) and post blood meal (PBM) phase. During the PV phase, egg maturation (vitellogenesis) is arrested at a premature stage until the blood meal-mediated uptake of amino acids and lipids (1–3). The PV phase, maintained by the juvenile hormone (JH), prepares the carbohydrate-consuming female mosquitoes for blood feeding by accumulating energy resources in the fat body, the major storage tissue in insects, and by activating their host-seeking behavior (4–9).…”

Section: Introductionmentioning

confidence: 99%

microRNA tissue atlas of the malaria mosquitoAnopheles gambiae

Lampe

Levashina

2017

Preprint

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Anopheles gambiae mosquitoes transmit the human malaria parasite Plasmodium falciparum, which causes the majority of fatal malaria cases worldwide. The hematophagous life style defines the mosquito reproductive biology and is exploited by P. falciparum for its own sexual reproduction and transmission. The two main phases of the mosquito reproductive cycle, pre-vitellogenic (PV) and post-blood meal (PBM) shape its capacity to transmit malaria. Transition between these phases is tightly coordinated to ensure homeostasis between mosquito tissues and successful reproduction. One layer of control is provided by microRNAs, well known regulators of blood meal digestion and egg development in Aedes mosquitoes. Here, we report a global overview of tissue-specific miRNA expression during the PV and PBM phases and identify miRNAs regulated during PV to PBM transition. The observed coordinated changes in the expression levels of a set of miRNAs in the energy-storing tissues suggest a role in the regulation of blood meal-induced metabolic changes.

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“…These included studies from the laboratories of Maestro (Spain), Léopold (France), Rewitz (Denmark), Sano (Japan), and Stocker (Switzerland). We also had a number of studies focusing on novel nutrient‐dependent phenomena and/or mechanisms (Danielsen, Moeller, & Rewitz, ; Koyama, Mendes, & Mirth, ; Mirth & Piper, ; Smykal & Raikhel, ), particularly by Uemura's laboratory (Japan) and Nishimura's laboratory (Japan). Size control was also studied by several groups, including by wet biology groups such as Bashirullah's laboratory (USA) and Koyama and Mirth's laboratory (Portugal‐Australia), and by theoretical biology groups such as Hironaka and Nishimura's laboratories (Japan).…”

Section: The Scientific Program Of Iihw2017mentioning

confidence: 99%

Assembly of insect hormone enthusiasts at Nasu Highland, Japan: Report of the 3rd International Insect Hormone (21st Ecdysone) Workshop

Niwa

Nishimura²

2017

Genes to Cells

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The 3rd International Insect Hormone (21st Ecdysone) Workshop (IIHW2017) was held in July 2017 at Nasu Highland, Japan. In the 40 years of the workshop's history, this was the first to be held in an Asian country. A total of 109 insect hormone researchers from 18 countries (62 overseas and 47 domestic participants) attended IIHW2017. During the workshop, all participants stayed on-site at the venue's hotel; this was ideal for fostering communication between participants, in particular, interactions between principal investigators and young scientists. The workshop featured one keynote, 64 oral, and 35 poster presentations spanning molecular biology, cell biology, developmental biology, neurobiology, chemical biology, physiology, and ecology of insect hormones, including ecdysteroids, juvenile hormones, and a variety of neuropeptides. The workshop provided an ideal platform for discussing insect hormone biology using not only the typical genetic model insect, the fruit fly Drosophila, but also a diversity of interesting insects, such as the silkworm, the red flour beetle, the cricket, the dragonfly, the social ant, the bloodsucking tick, and so on. The participants succeeded in sharing the latest knowledge in a wide range of insect hormone research fields and in joining active and constructive scientific discussions.

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Nutritional control of insect reproduction

Cited by 107 publications

References 64 publications

Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle of Aedes aegypti

Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle of Aedes aegypti

microRNA tissue atlas of the malaria mosquitoAnopheles gambiae

Assembly of insect hormone enthusiasts at Nasu Highland, Japan: Report of the 3rd International Insect Hormone (21st Ecdysone) Workshop

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