FRET detection of DNA sequence via electrostatic interaction of polycationic phenyleneethynylene dendrimer with DNA/PNA hybrid

Rashatasakhon, Paitoon; Vongnam, Kunnigar; Siripornnoppakhun, Warathip; Vilaivan, Tirayut; Sukwattanasinitt, Mongkol

doi:10.1016/j.talanta.2011.11.041

Cited by 17 publications

(10 citation statements)

References 39 publications

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“…The fluorescin-labeled pyrolidinyl PNA (Fl-acpcPNA), the energy acceptor is showed superior specificity in the DNA hybridization comparing to the classical PNA. The complementary DNA sequence is able to detect by the sensing In order to increase the PNA performance in the hybridization with complementary DNA, gold nanoparticle-PNA (AuNP-PNA) conjugates have produced surfactant polyoxyethylene (20) sorbitan monolaurate (Tween 20) [48,49]. The ability of AuNP-PNA conjugates have hybridized to its complementary DNA and verified using enzymelinked immunoassay.…”

Section: Solubility Of Pnamentioning

confidence: 99%

A Review of Peptide Nucleic Acid

Siddiquee¹,

Rovina²,

Azriah³

2015

Adv Tech Biol Med

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Peptide Nucleic Acid (PNA) is a nucleobase oligomer in which the whole backbone is mainly replaced by N-(2aminoethyl) glycine units. PNA is considered as DNA with a neutral peptide backbone due to negative charged sugar-phosphate backbone. It is chemically stable and resistant to hydrolytic cleavage. PNA can be categorized specific sequences of DNA and RNA according to Watson-Crick hydrogen bonding structure. Hybridization process showed high thermal stability and unique ionic strength effects. It is formed a stable PNA/DNA/PNA triplex with a looped-out DNA strand. PNA hybridization technology is promptly developed within in situ hybridization. In our review paper was elaborated the PNA superior hybridization characteristics, importance's of PNA and major applications of PNA in the diagnostic and pharmaceutical fields. And also PNA could be replaced DNA in uses as a probe for many investigation purposes. PNAs antisense activities have found in nerve cells and even in rats upon injection into the brain, and in Escherichia coli.

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Section: Solubility Of Pnamentioning

confidence: 99%

A Review of Peptide Nucleic Acid

Siddiquee¹,

Rovina²,

Azriah³

2015

Adv Tech Biol Med

View full text Add to dashboard Cite

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“…The use of a labeled PNA probe can be avoided by employing a fluorescence dye that can bind to PNA–DNA duplexes or triplexes, such as thiazole orange (TO) [ 123 ]. A polycationic dendritic fluorophore has also been used as a FRET donor to fluorescein-labeled pyrrolidinyl PNA probes giving a highly sequence-specific DNA detection at room temperature without requiring S1 digestion at a subnanomolar detection limit [ 124 ].…”

Section: Reviewmentioning

confidence: 99%

Fluorogenic PNA probes

Vilaivan¹

2018

Beilstein J. Org. Chem.

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Fluorogenic oligonucleotide probes that can produce a change in fluorescence signal upon binding to specific biomolecular targets, including nucleic acids as well as non-nucleic acid targets, such as proteins and small molecules, have applications in various important areas. These include diagnostics, drug development and as tools for studying biomolecular interactions in situ and in real time. The probes usually consist of a labeled oligonucleotide strand as a recognition element together with a mechanism for signal transduction that can translate the binding event into a measurable signal. While a number of strategies have been developed for the signal transduction, relatively little attention has been paid to the recognition element. Peptide nucleic acids (PNA) are DNA mimics with several favorable properties making them a potential alternative to natural nucleic acids for the development of fluorogenic probes, including their very strong and specific recognition and excellent chemical and biological stabilities in addition to their ability to bind to structured nucleic acid targets. In addition, the uncharged backbone of PNA allows for other unique designs that cannot be performed with oligonucleotides or analogues with negatively-charged backbones. This review aims to introduce the principle, showcase state-of-the-art technologies and update recent developments in the areas of fluorogenic PNA probes during the past 20 years.

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“…Such dyes have potential application as targeted probes in sensitive homogeneous (no wash) imaging. Inherently fluorescent cationic dendritic structures comprising primarily phenylene-ethynylene have been used as donors for the detection of peptide nucleic acid (PNA)/DNA hybridization, where the neutral PNA probe was labeled with a fluorescein acceptor [127]. Davis et al used inherently fluorescent cationic and anionic diphenylacetylene dendrimers doped into cellulose acetate to generate solid-state electrospun nanofiber sensor arrays for protein detection [128].…”

Section: Dendrimers and Polymer Macromoleculesmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

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The intrinsic sensitivity (r 6 dependence) of F€ orster (or fluorescence) resonance energy transfer (FRET) to nanoscale changes in the donor/acceptor separation distance has made FRET an invaluable biophysical tool in a variety of applications, ranging from studying the structure and conformation of proteins and nucleic acids to examining biomolecular interactions, including its use in in vitro and in vivo bioassays [1][2][3][4][5][6][7]. While the myriad of FRET configurations and techniques currently in use are covered throughout this book, here we focus primarily on the materials utilized as donor or acceptor probes in FRET rather than the process itself [3,5,8,9]. Our 2006 review paper on this topic serves as the foundation for this updated chapter [3]. The materials were divided into three main categories: organic materials that include "traditional" dye fluorophores, dark quenchers, polymers, and carbon nanomaterials (NMs); inorganic materials such as metal chelates, metal, and semiconductor nanocrystals; and fluorophores of biological origin such as fluorescent proteins (FPs), amino acids, and fluorescence generated from enzymatic bioluminescence (BL) and chemiluminescence (CL). These materials may function as FRET donors and/or acceptors, depending upon experimental design. Many of the new materials developed and/or new donor-acceptor probe combinations used address some of the inherent complications of more traditional FRET materials, including photobleaching, spectral cross talk, and direct excitation of the acceptor species, and examples of these will be highlighted and discussed throughout the chapter. Since the vast majority of FRET applications are biological in nature, they routinely involve some type of biomolecule labeling strategy, which ultimately plays a significant and fundamental role in the success and interpretation of the resulting FRET. Therefore, we begin the chapter with a brief discussion of the bioconjugation techniques commonly utilized for FRET and points to consider, followed by sections highlighting the current and emerging materials that are used, or have the potential to be used, in FRET applications.

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FRET detection of DNA sequence via electrostatic interaction of polycationic phenyleneethynylene dendrimer with DNA/PNA hybrid

Cited by 17 publications

References 39 publications

A Review of Peptide Nucleic Acid

A Review of Peptide Nucleic Acid

Fluorogenic PNA probes

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

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