FRET-based fluorescence probes for hydrolysis study and pig liver esterase activity

Yi, Long; Cao, Li; Liu, Liangliang; Xi, Zhen

doi:10.1016/j.tet.2008.06.051

Cited by 12 publications

(3 citation statements)

References 35 publications

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“…As shown in Figure S14, the K m = 34.92 μM, V max = 0.82 μM s À 1 , K cat = 4.12 s À 1 and K cat /K m = 1.18×10 5 M À 1 s À 1 , the turnover number (TON) was 706, which were consisted with the previous literatures. [14] Then, we studied the influence of human serum albumin (HSA) on the catalytic reaction between NIRÀ CE and CE. Absorption and emission spectra of probe NIRÀ CE remained unchanged in HSA (50 mg/mL) solution after incubation for 30 min (Figure S15).…”

Section: Methodsmentioning

confidence: 99%

Carboxylesterase Activatable Molecular Probe for Personalized Treatment Guidance by Analyte‐Induced Molecular Transformation

Li,

Liu,

Zhao

et al. 2024

Angewandte Chemie

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Accurate visualization of tumor microenvironment is of great significance for personalized medicine. Here, we develop a near‐infrared (NIR) fluorescence/photoacoustic (FL/PA) dual‐mode molecular probe (denoted as NIR‐CE) for distinguishing tumors based on carboxylesterase (CE) level by an analyte‐induced molecular transformation (AIMT) strategy. The recognition moiety for CE activity is the acetyl unit of NIR‐CE, generating the pre‐product, NIR‐CE‐OH, which undergoes spontaneous hydrogen atom exchange between the nitrogen atoms in the indole group and the phenol hydroxyl group, eventually transforming into NIR‐CE‐H. In cellular experiments and in vivo blind studies, the human hepatoma cells and tumors with high level of CE were successfully distinguished by both NIR FL and PA imaging. Our findings provide a new molecular imaging strategy for personalized treatment guidance.

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Section: Methodsmentioning

confidence: 99%

Carboxylesterase Activatable Molecular Probe for Personalized Treatment Guidance by Analyte‐Induced Molecular Transformation

Li,

Liu,

Zhao

et al. 2024

Angewandte Chemie

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“…Recently, the carboxylesterase (CaE) activity/level in human plasma was reported to be a novel serological biomarker candidate for HCC, as proven in previously reported research results [ 27 , 28 , 29 , 30 , 31 , 32 ]. Encouraged by this aspect, small-molecular fluorescent probes for CaE have been reported and applied in liver-related imaging and diagnosis [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 ]. Unfortunately, these probes suffer from the aggregation-caused quenching (ACQ) of fluorescence after their accumulation into cells or aggregation in physiological environments [ 43 , 44 , 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

A Fast-Response AIE-Active Ratiometric Fluorescent Probe for the Detection of Carboxylesterase

Xia

Liu

et al. 2022

Biosensors

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Hepatocellular carcinoma (HCC) is associated with a high mortality rate worldwide. The therapeutic outcomes can be significantly improved if diagnosis and treatment are initiated earlier in the disease process. Recently, the carboxylesterase (CaE) activity/level in human plasma was reported to be a novel serological biomarker candidate for HCC. In this article, we fabricated a new fluorescent probe with AIE characteristics for the rapid detection of CaE with a more reliable ratiometric response mode. The TCFISE probe showed high sensitivity (LOD: 93.0 μU/mL) and selectivity toward CaE. Furthermore, the good pH stability, superior resistance against photobleaching, and low cytotoxicity highlight the high potential of the TCFISE probe for application in the monitoring of CaE activity in complex biological samples and in live cells, tissues, and animals.

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“…Among them, biomolecular probes based on different fluorogenic substrates have received much attention due to the simplicity, speed, and sensitivity of their assays 10. Although these probes have been proven successful in carboxylesterase activity assays, the development of water‐soluble11 and synthetically readily accessible fluorogenic probes12 with improved assay sensitivity and selectivity is still desirable.…”

Section: Introductionmentioning

confidence: 99%

A Fluorogenic Probe with Aggregation‐Induced Emission Characteristics for Carboxylesterase Assay through Formation of Supramolecular Microfibers

Wang

Liu

et al. 2014

Chemistry An Asian Journal

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Herein, we report a novel fluorescent "light-up" probe useful for carboxylesterase assay that is based on a tetraphenylethylene derivative containing carboxylic ester groups. The specific cleavage of the carboxylic ester bonds by carboxylesterase results in the generation of a relatively hydrophobic moiety that self-assembles into supramolecular microfibers, thus giving rise to "turn-on" fluorescent signals. A high sensitivity towards carboxylesterase was achieved with a detection limit as low as 29 pM, which is much lower than the corresponding assays based on other fluorescent approaches.

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FRET-based fluorescence probes for hydrolysis study and pig liver esterase activity

Cited by 12 publications

References 35 publications

Carboxylesterase Activatable Molecular Probe for Personalized Treatment Guidance by Analyte‐Induced Molecular Transformation

Carboxylesterase Activatable Molecular Probe for Personalized Treatment Guidance by Analyte‐Induced Molecular Transformation

A Fast-Response AIE-Active Ratiometric Fluorescent Probe for the Detection of Carboxylesterase

A Fluorogenic Probe with Aggregation‐Induced Emission Characteristics for Carboxylesterase Assay through Formation of Supramolecular Microfibers

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