Freshly isolated cells and cell lines from the intestine as an in vitro model for toxicological studies

Zucco, Flavia

doi:10.1016/0887-2333(93)90035-4

Cited by 16 publications

(2 citation statements)

References 44 publications

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“…The suitability is due to their easy handling, quicker response and the presence of most of biochemical pathways similar to those present in the cells of higher animals. [9][10][11][36][37][38] Similarities in most of the tested responses and effects of aspirin between IEC, IEC-6 cell line and bacteria as observed in the present study further strengthen the notion that gut bacteria and the IEC-6 cell line can be used as an alternate to animals for screening of GI cellular toxicity caused by new NSAIDs.…”

Section: Discussionsupporting

confidence: 86%

Experimental impact of aspirin exposure on rat intestinal bacteria, epithelial cells and cell line

2010

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Aspirin, a commonly used therapeutic non-steroidal anti-inflammatory drug (NSAID) is known to cause gastric mucosal damage. Intestinal bacteria having a regulatory effect on intestinal homeostasis play significant role in NSAID-induced intestinal injury. Bacteria and specific cell lines are considered to be suitable for toxicity screening and testing of chemicals. Therefore, to evaluate and compare in vitro toxicity, cultures of rat intestinal epithelial cells (IEC), isolated bacteria and IEC-6 cell line were assessed for viability, morphometric analysis, membrane transport enzymes and structural constituents for membrane damage, dehydrogenase activity test for respiratory and energy producing processes and esterase activity test for intra- and extra-cellular degradation, following the post exposure to aspirin (0—50 µg mL- 1). Similar pattern of dose-dependent changes in these parameters were observed in three types of cells. Similar in situ effects on IEC validated the in vitro findings. These findings indicate that higher aspirin concentrations may alter cellular functions of IEC and gut bacteria. Furthermore, results suggest that gut bacteria and IEC-6 cell line can be used for the initial screening of gastrointestinal cellular toxicity caused by NSAIDs.

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Section: Discussionsupporting

confidence: 86%

Experimental impact of aspirin exposure on rat intestinal bacteria, epithelial cells and cell line

2010

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“…Intestinal cell culture systems have recently received increased attention as models for toxicological studies, as the intestine is the primary site of exposure to the toxicants in food [16]. We have reported that membrane‐perturbing toxicants such as benzalkonium chloride and Quillaja saponin induce a decrease in the TEER of Caco‐2 monolayers much earlier than they express their cytotoxic effects as reflected by lactate dehydrogenase release [17].…”

Section: Resultsmentioning

confidence: 99%

Purification and cDNA cloning of a protein derived from Flammulina velutipes that increases the permeability of the intestinal Caco‐2 cell monolayer

Watanabe

Narai

Shimizu

1999

European Journal of Biochemistry

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A new protein that decreases transepithelial electrical resistance (TEER) in the human intestinal Caco-2 cell monolayer was found in a water-soluble fraction of the mushroom Flammulina velutipes. This protein, termed TEER-decreasing protein (TDP), is not cytotoxic and does not induce cell detachment, but rapidly increases the tight junctional permeability for water-soluble marker substances such as Lucifer Yellow CH (M r 457) through the paracellular pathway. TDP was isolated and purified from the aqueous extract of F. velutipes by chromatographic means. Purified TDP was found to be a simple, nonglycosylated protein without intermolecular disulfide bonds, and the apparent molecular mass as estimated by SDS/PAGE and gel filtration is 30 kDa. It was revealed that the N-terminal amino-acid sequence of purified TDP is identical to the recently reported N-terminal sequence of flammutoxin, a membrane-perturbing hemolytic protein, for which the complete primary structure has not yet been reported [Tomita, T., Ishikawa, D., Noguchi, T., Katayama, E., and Hashimoto, Y. (1998) Biochem. J. 333, 24794±24799]. The cDNA coding for TDP was cloned by 5H and 3 H rapid amplification of cDNA ends. The ORF encodes a protein with 272 amino-acid residues showing no homology to known proteins. Relevant studies using TDP cDNA will provide insight into the structure±function relationships of membrane poreforming toxins.

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Intestinal Cell Culture Models

Sambruy¹,

Ferruzza²,

Ranaldi³

et al. 2001

Cell Culture Methods for in Vitro Toxicology

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Freshly isolated cells and cell lines from the intestine as an in vitro model for toxicological studies

Cited by 16 publications

References 44 publications

Experimental impact of aspirin exposure on rat intestinal bacteria, epithelial cells and cell line

Experimental impact of aspirin exposure on rat intestinal bacteria, epithelial cells and cell line

Purification and cDNA cloning of a protein derived from Flammulina velutipes that increases the permeability of the intestinal Caco‐2 cell monolayer

Intestinal Cell Culture Models

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