Frequent occurrence of oxacillin-susceptible<i>mecA</i>-positive<i>Staphylococcus aureus</i>(OS-MRSA) strains in two African countries: Table 1.

Conceição, Teresa; Coelho, Catarina; Lencastre, Herminia Garcez; Aires-de-Sousa, Marta

doi:10.1093/jac/dkv261

Cited by 30 publications

(40 citation statements)

References 28 publications

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“…We have also found strains belonging to ST-239 and ST-72 lineages which were reported from cases of human and animal infections [9], [44]-[45]. A strain with ST-88 lineage was identified, which is a major human MRSA clone from Africa and also has been reported in Asia, Europe and South America [20]. Interestingly, a recent report by Conceicao et al, 2015, also showed ST-88 as a major prevalent OS-MRSA clone causing human infections [20].…”

Section: Discussionmentioning

confidence: 99%

“…A strain with ST-88 lineage was identified, which is a major human MRSA clone from Africa and also has been reported in Asia, Europe and South America [20]. Interestingly, a recent report by Conceicao et al, 2015, also showed ST-88 as a major prevalent OS-MRSA clone causing human infections [20]. Therefore, the findings of the strains with MLST and spa type known in human infection in the current study may point towards transmission between human to animals.…”

Section: Discussionmentioning

confidence: 99%

“…aureus strains (OS-MRSA) has further complicated the scenario of diagnosis and treatment. The OS-MRSA strains have been reported in animal and human infections worldwide [10], [20–23], however, in India these strains were only reported in human infections [22]. The slow but increasing prevalence of OS-MRSA strains across the globe indicates that they may outnumber the existing MRSA strains in the near future and become a huge concern as they are not easily detectable [24].…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Characterization of Oxacillin Susceptible mecA-Positive Clinical Isolates of Staphylococcus aureus Causing Bovine Mastitis in India

et al. 2016

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Bovine mastitis caused by multidrug resistant Staphylococcus aureus is a huge problem reported worldwide, resulting in prolonged antibiotic treatment and death of livestock. The current study is focused on surveillance of antibiotic susceptibility along with genotypic and phenotypic characterization of the pathogenic S. aureus strains causing mastitis in India. One hundred and sixty seven milk samples were collected from mastitis-affected cows from different farms in India resulting in thirty nine isolated S. aureus strains. Antibiotic sensitivity profiling revealed the majority of the strains (n = 24) to be multidrug resistant and eleven strains showed reduced susceptibility to vancomycin (MICs = 2μg/ml). All strains were oxacillin sensitive, but 19 strains were positive for the mecA gene, which revealed the occurrence of oxacillin susceptible mecA positive strains (OS-MRSA) for the first time from India. Additionally, 32 strains were positive for the pvl gene, a virulence determinant; of these 17 were also OS-MRSA strains. Molecular characterization based on multilocus sequence typing (MLST), spa typing, agr typing and SCCmec classification revealed strains belonging to different groups. Moreover, strains showed spa types (t2526, t9602) and MLST sequence types, ST-72, ST-88 and ST-239 which have been earlier reported in human infections. The prevalence of OS-MRSA strains indicates the importance of including both the genetic and phenotypic tests in characterizing S. aureus strains. Increased genotypic variability with strain related to human infections and pvl positive isolates indicates a worrisome situation with the possibility of bilateral transfer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and Characterization of Oxacillin Susceptible mecA-Positive Clinical Isolates of Staphylococcus aureus Causing Bovine Mastitis in India

et al. 2016

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“…All the 77 MRSA strains were resistant to SXT, FOX, and PEN . In a study to identify OXA‐susceptible mecA ‐positive S. aureus (OS‐MRSA) for the first time in Africa, a prevalence of 17.7% was detected among healthy healthcare workers in Angola and São Tomé and Príncipe, making them potential OS‐MRSA reservoirs . The OS‐MRSA isolates displayed MDR (Table S2, online only) and were characterized as being ST88 ( n = 15) and ST8 ( n = 9).…”

Section: Resultsmentioning

confidence: 99%

“…MRSA prevalence of 26.9% and 25.5% was reported in nasal swabs in 2014 and 2015, respectively, in São Tomé and Príncipe. Additionally, a high prevalence of OS‐MRSA was reported in both São Tomé and Príncipe and Angola . Dominant MRSA clones circulating in São Tomé and Príncipe include ST8 ( n = 34), ST5 ( n = 13), ST88 ( n = 28), ST5 ( n = 3), ST1 ( n = 2), and ST105 ( n = 2) .…”

Section: Resultsmentioning

confidence: 99%

Molecular epidemiology and mechanisms of antibiotic resistance in Enterococcus spp., Staphylococcus spp., and Streptococcus spp. in Africa: a systematic review from a One Health perspective

Sekyere

Mensah

2019

Annals of the New York Academy of Sciences

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A systematic review of antibiotic‐resistant Gram‐positive bacteria in Africa from a One Health perspective is lacking. Here, we report result from a search for English‐language articles on the resistance mechanisms and clonality of Gram‐positive bacteria in Africa between 2007 and 2019 reported in PubMed, Web of Science, ScienceDirect, and African Journals OnLine; 172 studies from 22 different African countries were identified. Resistance genes, such as mecA, erm(B), erm(C), tet(M), tet(K), tet(L), vanB, vanA, vanC, and tet(O), were found to be common. Staphylococcus spp., Enterococcus spp., and Streptococcus spp. were the main species reported by the studies, with clones such as Staphylococcus aureus ST5 (n = 218 isolates), ST8 (n = 127 isolates), ST80 (n = 133 isolates), and ST88 (n = 117 isolates), and mobile genetic elements such as IS16 (n = 28 isolates), IS256 (n = 96), Tn916 (n = 107 isolates), and SCCmec (n = 4437 isolates) identified. SCCmec IV (n = 747 isolates) was predominant, followed by SCCmec III (n = 305 isolates), SCCmec II (n = 163 isolates), SCCmec V (n = 135 isolates), and SCCmec I (n = 79 isolates). Resistance to penicillin (n = 5926 isolates), tetracycline (n = 5300 isolates), erythromycin (n = 5151 isolates), rifampicin (n = 3823 isolates), gentamycin (n = 3494 isolates), sulfamethoxazole/trimethoprim (n = 3089 isolates), and ciprofloxacin (n = 2746 isolates) was common in most reports from 22 countries. Clonal dissemination of resistance across countries and between humans, animals, and the environment was observed. Resistance rates ranged from 1.4% to 100% for 15 of the studies; 10 were One Health–related studies. Strict infection control measures, antimicrobial stewardship, and periodic One Health epidemiological surveillance studies are needed to monitor and contain the threat of increasing antibiotic resistance in Africa.

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Molecular epidemiology of Staphylococcus aureus from Lambaréné, Gabon

Okuda

Toepfner

Alabi

et al. 2016

Eur J Clin Microbiol Infect Dis

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While there is an abundance of data on the epidemiology and molecular typing of Staphylococcus aureus, especially those carrying Panton-Valentine leucocidin (PVL) genes or mecA from Western Europe, Northern America and Australia, comparably few studies target African strains. In this study, we characterised genes associated with virulence and resistance, as well the phylogenetic background of S. aureus from healthy carriers and outpatients in Gabon. In total, 103 isolates from 96 study participants were characterised. Seventy-nine isolates originated from throat swabs and 24 isolates from skin lesions. Three isolates carried mecA, although only one, belonging to CC8-MRSA-IV [PVL+] 'USA300', was found to be phenotypically oxacillin-resistant; two CC88-MRSA-IV isolates appeared to be oxacillin-susceptible. PVL genes were common, with a total of 44 isolates (43 %) found to be PVL-positive. CC15-MSSA [PVL+] (n = 29) and CC152-MSSA [PVL+] (n = 9) were the predominant clones among the PVL-positive isolates. Among PVL-negative isolates, CC5-MSSA (n = 12), CC101-MSSA (n = 10) and CC15 (n = 9) were the most frequent. A hitherto undescribed multilocus sequence type of S. schweitzeri was detected twice in unrelated patients. The data emphasise a need for further studies on the role of PVL in African populations and the clinical significance of S. schweitzeri.

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Frequent occurrence of oxacillin-susceptiblemecA-positiveStaphylococcus aureus(OS-MRSA) strains in two African countries: Table 1.

Cited by 30 publications

References 28 publications

Prevalence and Characterization of Oxacillin Susceptible mecA-Positive Clinical Isolates of Staphylococcus aureus Causing Bovine Mastitis in India

Prevalence and Characterization of Oxacillin Susceptible mecA-Positive Clinical Isolates of Staphylococcus aureus Causing Bovine Mastitis in India

Molecular epidemiology and mechanisms of antibiotic resistance in Enterococcus spp., Staphylococcus spp., and Streptococcus spp. in Africa: a systematic review from a One Health perspective

Molecular epidemiology of Staphylococcus aureus from Lambaréné, Gabon

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