Frequent epigenetic inactivation of chromosome 3p candidate tumor suppressor genes in gallbladder carcinoma

Riquelme, Erick; Tang, Moying; Báez, Sergio; Díaz, Alejandro; Pruyas, M; Wistuba, Ignacio I.; Corvalán, Alejandro

doi:10.1016/j.canlet.2006.09.019

Cited by 49 publications

(46 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DNA was treated with sodium bisulfite as described previously (16). Briefly, 1 Ag of genomic DNA was denatured by incubation with 0.2 mol/L NaOH for 10 min at 37jC.…”

Section: Methodsmentioning

confidence: 99%

Reprimo as a Potential Biomarker for Early Detection in Gastric Cancer

Bernal¹,

Aguayo

Villarroel

et al. 2008

Clinical Cancer Research

107

View full text Add to dashboard Cite

Purpose: Gastric cancer is a curable disease if diagnosed at early stage. However, most cases are diagnosed at advanced stage because of the lack of screening programs. Therefore, the identification of plasma biomarkers for early detection is necessary. Experimental Design: To search for these biomarkers, we evaluated the DNA methylation patterns of 24 genes by Methylation-specific PCR in primary tissues from 32 retrospectively collected gastric cancer cases (testing group). Correlation between methylation and gene expression was evaluated in the MKN-45 cell line after treatment with 5-aza-2 ¶-deoxycytidine. The most frequently hypermethylated genes were next evaluated in primary tissues and plasma samples from 43 prospectively collected gastric cancer cases as well as plasma samples from 31 asymptomatic age-and gender-matched controls (validation group). Results: In the testing group, 11 genes were hypermethylated in at least 50% of cases (APC, SHP1, E-cadherin, ER, Reprimo, SEMA3B, 3OST2, p14, p15, DAPK , and p16). Eight genes (BRCA1, p73, RARb, hMLH1, RIZI, RUNX3, MGMT, and TIMP3) were statistically associated with a particular variant of gastric cancer, the signet-ring cell type (P = 0.03). Seven genes (APC, SHP1, E-cadherin, ER, Reprimo, SEMA3B, and 3OST2) were next evaluated in the validation group. We confirm the high frequency of methylation in primary tumors for all seven genes. However, only APC and Reprimo were frequently methylated in pair plasma samples. In asymptomatic controls, only Reprimo was infrequently methylated in comparison with plasma from gastric cancer cases (P < 0.001). Conclusion:Our results identified specific methylation profile associated to signet-ring cell-type histology and aberrant hypermethylation of Reprimo as a potential biomarker for early detection of gastric cancer.

show abstract

“…DNA was treated with sodium bisulfite as described previously (16). Briefly, 1 Ag of genomic DNA was denatured by incubation with 0.2 mol/L NaOH for 10 min at 37jC.…”

Section: Methodsmentioning

confidence: 99%

Reprimo as a Potential Biomarker for Early Detection in Gastric Cancer

Bernal¹,

Aguayo

Villarroel

et al. 2008

Clinical Cancer Research

107

View full text Add to dashboard Cite

show abstract

“…LOH is a surrogate marked for chromosomal deletions. Riquelme et al [69] carried out analysis of methylation status of six candidate TSGs located in chromosome 3p, including DUTT1 (3p12), FHIT (3p14.2), BLU, RASSF1A and SEMA3B (3p21.3) and hMLH1 (3p21.3) in 50 GBC samples. A very high frequency of methylation was detected in SEMA3B (46/50, 92 %) and FHIT (33/50, 66 %), intermediate incidences in BLU (13/50, 26 %) and DUTT1 (11/50, 22 %) and very low frequencies in RASSF1A (4/50, 8 %) and hMLH1 (2/50, 4 %).…”

Section: Epigenetic Changes In Gbcmentioning

confidence: 99%

Epigenetic Changes in Carcinogenesis of Gallbladder

Tewari

Agarwal

Mishra

et al. 2013

Indian J Surg Oncol

View full text Add to dashboard Cite

Gallbladder cancer (GBC) is a lethal and a common malignancy affecting mostly females. There are restricted high incidence pockets across the world and in northern India highest incidence of GBC is reported from the Gangetic belt. The etiology of this disease remains largely unknown though several risk factors have been stated. The genetic aberrations in GBC involving mutations in tumor suppressor genes and oncogenes have been reported in literature. However, there is scarcity of data regarding epigenetic changes that may also be involved in gallbladder carcinogenesis. This review attempts to summarize our current understanding of the epigenetic changes in GBC.

show abstract

“…Promoter CpG island hypermethylation is coincident with loss of BLU/ZMYND10 expression, which can be restored following treatment with the DNA demethylating agent 5-aza-2 0 -deoxycytidine (5azaDC) (Agathanggelou et al, 2003b;Hesson et al, 2004;Qiu et al, 2004;Yau et al, 2006;Yi Lo et al, 2006). BLU/ZMYND10 hypermethylation and downregulation has been described as a frequent event in primary tumours such as glioma (80%), cervical squamous cell carcinomas (77%), NPC (66%), neuroblastoma (41-70%) and NSCLC (19-43%) with lower frequencies observed in gall bladder carcinomas (26%), ependymomas (13.6%) and SCLC (14%) (Agathanggelou et al, 2003b;Hesson et al, 2004;Qiu et al, 2004;Abe et al, 2005;Ito et al, 2005;Marsit et al, 2005;Lai et al, 2006;Michalowski et al, 2006;Riquelme et al, 2007). In some tumour types, BLU/ZMYND10 methylation is an early event detectable in stage II glioma and stage I/II cervical squamous cell carcinomas (at frequencies of 88 and 85/68%, respectively) Lai et al, 2006).…”

Section: Rassf1mentioning

confidence: 99%