Frequency of plasmid-mediated AmpC in Enterobacteriaceae isolated in a Brazilian Teaching Hospital

Campana, Eloiza Helena; Barbosa, Paula P.; Fehlberg, Lorena Cristina Corrêa; Gales, Ana Cristina

doi:10.1590/s1517-83822013000200023

Cited by 11 publications

(7 citation statements)

References 13 publications

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“…FOX-5 like and CMY-2-like were the first pAmpC beta-lactamases reported in Brazilian isolates and were detected in E. coli from patients in hospitals [10, 11]. Studies have shown an increase in the frequency of pAmpC in human clinical setting, but few studies have described the frequency of pAmpC in Enterobacteriaceae in Brazil [6, 11, 28, 29].…”

Section: Discussionmentioning

confidence: 99%

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

et al. 2019

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Background Food-producing animals, mainly poultry, have been associated with the maintenance and dissemination of antibiotic-resistant bacteria, such as plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae, to humans, thus impacting food safety. Many studies have shown that Escherichia coli strains isolated from poultry and humans infections share identical cephalosporin resistance, suggesting that transmission of resistance from poultry meat to humans may occur. The aim of this study was to characterize pAmpC-producing E. coli strains isolated from chicken carcasses and human infection in a restrict area and to determine their antimicrobial resistance profiles, and molecular type by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Results A total of 14 pAmpC-producing E. coli strains were isolated, including eight strains from chicken carcasses and six strains from human infections (from urine, tissue and secretion). The bla CMY-2 gene was identified in all pAmpC-producing E. coli strains by polymerase chain reaction (PCR) and DNA sequencing. High percentages of strains resistant to tetracycline, nalidixic acid and sulfamethoxazole-trimethoprim (78–92%) were detected, all of which were considered multidrug-resistant. Among the non-beta-lactam resistance genes, the majority of the strains showed tet A, tet B, sul I and sul II. No strain was considered an extended-spectrum beta-lactamases (ESBL) producer, and the bla TEM-1 gene was found in 2 strains isolated from human infection. Six strains from chicken carcasses and four strains from humans infections were linked to an ISE cp1 -like element. Through MLST, 11 sequence types were found. Three strains isolated from human infection and one strain isolated from chicken carcasses belonged to the same sequence type (ST354). However, considerable heterogeneity between the strains from chicken carcasses and humans was confirmed by PFGE analysis. Conclusion This study showed the prevalence of E. coli strains producing bla CMY-2 linked to ISE cp1 that were present in both chickens and humans in a restricted area. Our results also suggest the presence of a highly diverse strains that harbor pAmpC, indicating no clonal dissemination. Therefore, continuous monitoring and comparative analyses of resistant bacteria from humans and food-producing animals are needed.

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Section: Discussionmentioning

confidence: 99%

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

et al. 2019

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“…Studies have related the presence of the CIT group in poultry in other countries [ 4 , 12 , 13 ]. In Brazil, the presence of plasmid-mediated AmpC-producing in human isolates has been sporadically reported [ 38 , 39 ]. The presence of 11 AmpC-producing strains indicates the importance of studies both in human and in veterinary clinical practice.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Antibiotic Resistance and Virulence Factors amongEscherichia coliIsolated from Conventional and Free-Range Poultry

Koga

Scandorieiro

Vespero

et al. 2015

BioMed Research International

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Microbiological contamination in commercial poultry production has caused concerns for human health because of both the presence of pathogenic microorganisms and the increase in antimicrobial resistance in bacterial strains that can cause treatment failure of human infections. The aim of our study was to analyze the profile of antimicrobial resistance and virulence factors of E. coli isolates from chicken carcasses obtained from different farming systems (conventional and free-range poultry). A total of 156 E. coli strains were isolated and characterized for genes encoding virulence factors described in extraintestinal pathogenic E. coli (ExPEC). Antimicrobial susceptibility testing was performed for 15 antimicrobials, and strains were confirmed as extended spectrum of β-lactamases- (ESBLs-) producing E. coli by phenotypic and genotypic tests. The results indicated that strains from free-range poultry have fewer virulence factors than strains from conventional poultry. Strains from conventionally raised chickens had a higher frequency of antimicrobial resistance for all antibiotics tested and also exhibited genes encoding ESBL and AmpC, unlike free-range poultry isolates, which did not. Group 2 CTX-M and CIT were the most prevalent ESBL and AmpC genes, respectively. The farming systems of poultries can be related with the frequency of virulence factors and resistance to antimicrobials in bacteria.

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“…A study conducted in a public tertiary hospital from São Paulo included 41 E. coli , five Klebsiella oxytoca , 65 Klebsiella pneumoniae , 18 P. mirabilis , and four Salmonella spp. detected during the period from January and July 2006 and found a 0.75% plasmid-mediated AmpC production rate and a single isolate producing a CMY-2-like enzyme was identified 45 . The most recent study on pAmpCs in Brazil evaluated the frequency of plasmid-mediated AmpCs in E. coli isolated from urine cultures from both outpatients and inpatients.…”

Section: Plasmid-mediated Ampcsmentioning

confidence: 99%

Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins

Sampaio

Gales

2016

Brazilian Journal of Microbiology

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During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96.2%) in carbapenem resistant K. pneumoniae. To date KPC-2 is the only variant reported in Brazil. Polymyxin B resistance in KPC-2-producing K. pneumoniae has come to an alarming rate of 27.1% in 2015 in São Paulo, the largest city in Brazil. New Delhi metallo-β-lactamase was detected in Brazil in 2013, has been reported in different Brazilian states but are not widely disseminated. Antimicrobial resistance in Enterobacteriaceae in Brazil is a very serious problem that needs urgent actions which includes both more strict adherence to infection control measures and more judicious use of antimicrobials.

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Frequency of plasmid-mediated AmpC in Enterobacteriaceae isolated in a Brazilian Teaching Hospital

Cited by 11 publications

References 13 publications

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

Comparison of Antibiotic Resistance and Virulence Factors amongEscherichia coliIsolated from Conventional and Free-Range Poultry

Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins

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