Frequency of micronuclei in peripheral blood lymphocytes from subjects occupationally exposed to low levels of ionizing radiation

Joseph, Lebana J.; Patwardhan, Uma N.; Samuel, A.M.

doi:10.1016/j.mrgentox.2004.07.012

Cited by 47 publications

(27 citation statements)

References 31 publications

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“…However, other authors have shown such an association [24–26]. In addition, the present study did not find any association between micronucleus occurrence and gender, concordant with the results from some studies [23, 27, 28], although this association has been described by others [29–31]. Like Bloching et al [26], the present study did not find any association between micronucleus occurrence and oral hygiene.…”

Section: Discussionsupporting

confidence: 92%

Chromosomal Damage and Apoptosis in Exfoliated Buccal Cells from Individuals with Oral Cancer

Dórea

Meireles

Lessa

et al. 2012

International Journal of Dentistry

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This study aimed to investigate cytological abnormalities indicative of chromosome damage (micronuclei) and apoptosis (karyorrhexis, pyknosis, and condensed chromatin) in exfoliated cells from the buccal mucosa of patients with oral cancer and control subjects. The sample included twenty individuals with oral cancer and forty individuals with normal buccal mucosa. Material was collected from the cheek epithelium in areas with lesions and areas without abnormalities. A minimum of one thousand cells was analyzed. Micronuclei were found significantly more frequently in cells collected from lesions than in cells from normal areas, independent of the presence/absence of cancer (P < 0.0001). They were also significantly more frequent in smokers and in mouthwash users (P < 0.0001). Apoptosis occurred significantly less frequently in individuals with oral cancer (P < 0.0001). These results show that oral cancer is associated with higher frequency of chromosomal damage and suggest that apoptosis is compromised in the buccal cells of individuals with this kind of neoplasia.

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Section: Discussionsupporting

confidence: 92%

Chromosomal Damage and Apoptosis in Exfoliated Buccal Cells from Individuals with Oral Cancer

Dórea

Meireles

Lessa

et al. 2012

International Journal of Dentistry

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“…Studies suggest that this process is associated with an increase in genomic instability due to the reduced capacity to repair damaged DNA. 1,2 It has been reported that, in old age, biomarkers of genomic instability, such as micronuclei (MN), are more common in the peripheral lymphocytes 3,4 and exfoliated epithelial cells. 5,6 The accumulation of DNA damage is a significant event in the aging of cells.…”

Section: Introductionmentioning

confidence: 99%

Effects of age on the frequency of micronuclei and degenerative nuclear abnormalities

Ferraz

Neto

Cerqueira

et al. 2016

Rev. bras. geriatr. gerontol.

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The effects of aging, gender and lifestyle factors on inducing chromosomal damage (micronuclei) and nuclear degenerative changes were assessed using the micronucleus test on exfoliated cells of the oral mucosa. The sample included 80 healthy subjects divided into four groups according to age and gender: men and women aged 19-29 years (M19, W19) and men and women aged over sixty years (M60, W60). An interview questionnaire was used to characterize the sample and to determine an index reflecting lifestyle (HLI). The frequency of micronuclei and nuclear degenerative changes was significantly higher among the elderly ( p<0.001) and did not differ by gender among young people ( p>0.05). The occurrence of micronuclei was similar among elderly men and women ( p>0.10), but karyorrhexis and karyolysis were more frequent among men ( p<0.005 and p<0.025, respectively), who also had a lower HLI than the other groups ( p<0.0004). The results of the study indicate that age is the main factor associated with the induction of genetic material damage.

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“…This finding is in agreement with the study of Gourabi and Mozdarani (1998), who observed that the distribution and frequency of MN in lymphocytes of individuals occupationally exposed to radiation and controls were not different when genders were compared. Joseph et al (2004) also found no significant differences between both genders regarding the MN frequencies of individuals occupationally exposed to radiation.…”

Section: Discussionmentioning

confidence: 65%

Cytogenetic biomonitoring of inhabitants of a large uranium mineralization area: the municipalities of Monte Alegre, Prainha, and Alenquer, in the State of Pará, Brazil

et al. 2010

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Uranium is a natural radioactive metallic element; its effect on the organism is cumulative, and chronic exposure to this element can induce carcinogenesis. Three cities of the Amazon region-Monte Alegre, Prainha, and Alenquer-in North Brazil, are located in one of the largest uranium mineralization areas of the world. Radon is a radioactive gas, part of uranium decay series and readily diffuses through rock. In Monte Alegre, most of the houses are built of rocks removed from the Earth's crust in the forest, where the uranium reserves lie. The objective of the present work is to determine the presence or absence of genotoxicity and risk of carcinogenesis induced by natural exposure to uranium and radon in the populations of these three cities. The frequency of micronuclei (MN) and chromosomal aberrations (CA) showed no statistically significant differences between the control population and the three study populations (P > 0.05). MN was also analyzed using the fluorescence in situ hybridization (FISH) technique, with a centromere-specific probe. No clastogenic and/or aneugenic effects were found in the populations. Using FISH analysis, other carcinogenesis biomarkers were analyzed, but neither the presence of the IGH/BCL2 translocation nor an amplification of the MYC gene and 22q21 region was detected. Clastogenicity and DNA damage were also not found in the populations analyzed using the alkaline comet assay. The mitotic index showed no cytotoxicity in the analyzed individuals' lymphocytes. Once we do not have data concerning radiation doses from other sources, such as cosmic rays, potassium, thorium, or anthropogenic sources, it is hard to determine if uranium emissions in this geographic region where our study population lives are too low to cause significant DNA damage. Regardless, genetic analyses suggest that the radiation in our study area is not high enough to induce DNA alterations or to interfere with mitotic apparatus formation. It is also possible that damages caused by radiation doses undergo cellular repair.

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Frequency of micronuclei in peripheral blood lymphocytes from subjects occupationally exposed to low levels of ionizing radiation

Cited by 47 publications

References 31 publications

Chromosomal Damage and Apoptosis in Exfoliated Buccal Cells from Individuals with Oral Cancer

Chromosomal Damage and Apoptosis in Exfoliated Buccal Cells from Individuals with Oral Cancer

Effects of age on the frequency of micronuclei and degenerative nuclear abnormalities

Cytogenetic biomonitoring of inhabitants of a large uranium mineralization area: the municipalities of Monte Alegre, Prainha, and Alenquer, in the State of Pará, Brazil

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