Frequency of HLA-G exon 8 polymorphisms and kidney allograft outcome in Iranian population

Aghdaie, Mahdokht Hossein; Azarpira, Negar; Kazemi, Kourosh; Geramizadeh, Bita; Darai, Masumeh; Malekhoseini, Seid Ali

doi:10.1007/s11033-010-0470-y

Cited by 20 publications

(9 citation statements)

References 31 publications

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“…No statistically significant differences were found between the HLA-G 14 bp deletion/insertion allele and genotypes between kidney transplant rejection group and non-rejection group. This observation is in agreement with data reported by others [29,30]. In the group with non kidney graft rejection higher frequencies of homozygous -14/-14 bp genotype were observed in comparison to recipients with acute rejection, as confirmed by others [21,22].…”

Section: Discussionsupporting

confidence: 93%

Impact of HLA-G 14 bp polymorphism and soluble HLA-G level on kidney graft outcome

Ďurmanová

Bandžuchová²,

Žilinská

et al. 2016

Open Life Sciences

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Human leukocyte antigen G (HLA-G) is a non-classical HLA class I protein with various immunosuppressive functions. Besides its profound effect to induce fetal tolerance, HLA-G has been also found to enhance graft acceptance. The aim of the study was to analyse the association between HLA-G 14 bp insertion/deletion polymorphism, soluble HLA-G level and kidney graft outcome in the Slovak population. We investigated 69 kidney transplant recipients aged 27–65 years. Out of this group, 37 recipients developed acute rejection, confirmed by biopsy, and 32 patients had stable allograft function. Plasma was obtained from recipients at 1 day before transplantation and analyzed by ELISA. Genotyping of HLA-G polymorphism was performed by PCR. Significantly higher pre-transplantation levels of sHLA-G were found in the group with stable allograft function in comparison to group with acute rejection (P = 0.0409). In the homozygous −14/−14 recipients with stable allograft function, significantly higher values of sHLA-G were determined in comparison to the recipients with acute rejection (P = 0.0052). The study revealed an association between 14 bp deletion polymorphism and soluble HLA-G level that is proportional to kidney graft acceptance. It is suggested that pre-transplantation levels of soluble HLA-G should be monitored as additional marker to predict kidney graft outcome.

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Section: Discussionsupporting

confidence: 93%

Impact of HLA-G 14 bp polymorphism and soluble HLA-G level on kidney graft outcome

Ďurmanová

Bandžuchová²,

Žilinská

et al. 2016

Open Life Sciences

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“…Moreover, HLA-G 14 bp insertion/deletion polymorphism (rs16375) was amplified by polymerase chain reaction (PCR) [21] with the forward primer HLAG1 5′-GTGATGGGCTGTTTAAAGTGTCACC-3′ and the reverse primer HLAG2 5′-GGAAGGAATGCAGTTCAGCATGA-3′.…”

Section: Methodsmentioning

confidence: 99%

HLA-G Polymorphism (rs16375) and Acute Rejection in Liver Transplant Recipients

et al. 2014

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Background. HLA-G molecules exhibit immunomodulatory properties that can delay graft rejection. The 14 bp insertion/deletion polymorphism (INDEL) (rs16375) influences the stability of final HLA-G mRNA and its soluble isoforms. Objective. The present study aimed to investigate the possible association between this polymorphism and the incidence of acute rejection in Iranian liver transplant recipients. Methods. Different genotypes were evaluated by PCR. The patients who had acute rejection within 6 months after transplantation were classified as acute rejection (AR) group, while others were considered as nonacute rejection (NAR) group. Results. Among the recipients, 21 patients (21%) had at least one episode of AR, while the other 79 patients (79%) had normal liver function. No significant differences were found between the two groups regarding sex, MELD score, and primary liver disease. Also, no difference was observed concerning rs16375 genotype and allele frequency (P = 0.44, OR: 0.69; CI: 0.21–2.10). Conclusion. The study results revealed no significant difference between the AR and the NAR groups regarding the 14 bp INDEL genotypes and alleles. Further studies are recommended to be conducted on other polymorphic sites as well as monitoring of serum HLA-G concentration in order to ascertain the potential implications of this marker in our population.

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“…Included characteristics were the name of the first author, year of publication, country of study origin, source of control group, sample size, and HardyWeinberg equilibrium (Table 1). [4][5][6][7][8][9] Statistical analyses All statistical analyses were performed with software (Stata 11.0, StataCorp, College Station, TX, USA) using 2-sided tests. The chi-square test was used to compare genotype (likelihood ratio P value) and allele frequencies (continuity correction P value) between the kidney recipient and healthy control groups.…”

Section: Data Extractionmentioning

confidence: 99%

“…Therefore, 5 studies that included a total of 907 kidney transplant recipients (rejection, 271 patients; no rejection, 636 patients) and 887 healthy control subjects were included in the meta-analysis (Table 1). [5][6][7][8][9] According to the Newcastle-Ottawa quality assessment scale, all the studies were of high quality ( Table 2).…”

Section: Study Identification and Characteristicsmentioning

confidence: 99%

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2014

Exp Clin Transplant

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Objectives: The human leukocyte antigen-G may have a positive role in graft acceptance in human organ transplant. Several studies have reported an association between the human leukocyte antigen-G-14-base-pair-insertion/deletion polymorphism and risk of developing kidney graft rejection, but the results are inconclusive. We performed a metaanalysis to evaluate this association. Conclusions: There may be no association between 14-base-pair polymorphisms and risk of developing kidney allograft rejection. Additional studies with larger sample size and better study design are justified.

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Frequency of HLA-G exon 8 polymorphisms and kidney allograft outcome in Iranian population

Cited by 20 publications

References 31 publications

Impact of HLA-G 14 bp polymorphism and soluble HLA-G level on kidney graft outcome

Impact of HLA-G 14 bp polymorphism and soluble HLA-G level on kidney graft outcome

HLA-G Polymorphism (rs16375) and Acute Rejection in Liver Transplant Recipients

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