HLA-G Polymorphism (rs16375) and Acute Rejection in Liver Transplant Recipients

Abstract

Background. HLA-G molecules exhibit immunomodulatory properties that can delay graft rejection. The 14 bp insertion/deletion polymorphism (INDEL) (rs16375) influences the stability of final HLA-G mRNA and its soluble isoforms. Objective. The present study aimed to investigate the possible association between this polymorphism and the incidence of acute rejection in Iranian liver transplant recipients. Methods. Different genotypes were evaluated by PCR. The patients who had acute rejection within 6 months after transplantation were classified as acute rejection (AR) group, while others were considered as nonacute rejection (NAR) group. Results. Among the recipients, 21 patients (21%) had at least one episode of AR, while the other 79 patients (79%) had normal liver function. No significant differences were found between the two groups regarding sex, MELD score, and primary liver disease. Also, no difference was observed concerning rs16375 genotype and allele frequency (P = 0.44, OR: 0.69; CI: 0.21–2.10). Conclusion. The study results revealed no significant difference between the AR and the NAR groups regarding the 14 bp INDEL genotypes and alleles. Further studies are recommended to be conducted on other polymorphic sites as well as monitoring of serum HLA-G concentration in order to ascertain the potential implications of this marker in our population.

Identification of the role of new genetic biomarkers in susceptibility and pathogenesis of spondyloarthropathies in Morocco.

“…Four gene polymorphisms in CTLA-4 CT60 region (dbSNP rs182533364 C/T, rs345031880 T/C, rs3087243 G/A, rs11571319 G/A) were analyzed by the SBT technique, using primers already described in literature (Azarpira et al, 2010;Canossi et al, 2013). Typing was obtained by alignment of the processed sequences with exon 4 sequences of the human CTLA-4 gene reference from the Genebank.…”

Tolerogenic Dendritic Cells on Transplantation: Immunotherapy Based on Second Signal Blockage

“…This molecule has local immunomodulatory properties, due to its limited polymorphism, contributing to the survival of allogeneic liver transplants. HLA-G1 to G4 are membrane-bound molecules while HLA-G5 to G7 are soluble molecules (sHLA-G) [ 87 , 88 ]. HLA-G was previously identified as a naturally occurring tolerance-inducing molecule.…”

HLA-G 14 bp INSERTION/ DELETION POLYMORPHISM IN RECURRENT PREGNANCY LOSS AMONG A SAMPLE OF IRAQI WOMEN.

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