Frequency of heavy-metal resistance in bacteria from inpatients in Japan

Nakahara, Hideomi; Ishikawa, Tomoaki; Sarai, Yasunaga; Kondo, Isamu

doi:10.1038/266165a0

Cited by 102 publications

(47 citation statements)

References 20 publications

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“…Mercury resistance was detected at the high frequency of 43% of the isolates (Table 3). Nakahara et al (16,17) reported finding mercury resistance at a high frequency in E. coli (57%), Klebsiella pneumoniae (66%), Pseudomonas aeruginosa (75%), and Staphylococcus aureus (36%) isolates of human origin in Japan. We (23) reported that all of the triple (SaSmAp) resistance determinants tested were associated with mercury resistance and were conjugative in Bordetella bronchiseptica isolates from pigs.…”

Section: Discussionmentioning

confidence: 99%

Appearance of Enterotoxigenic Escherichia coli in Piglets with Diarrhea in Connection with Feed Changes

Shimizu

Terashima

1982

Microbiology and Immunology

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Twelve weaned piglets (3-week-old) were divided into three groups according to the time of feed change and observed for diarrhea during the time they were 3 to 8 weeks of age. A total of 553 strains of Escherichia coli were isolated from rectal fecal samples and examined for heat-labile (LT) and heat-stable (ST) enterotoxins, pilus antigens (K88, K99, and 987P), hemolysin (Hly), raffinose utilization (Raf) and drug resistance. Enterotoxigenic and/or hemolytic E. coli strains appeared in the rectal feces of 5-to 6-week-old piglets with diarrhea in connection with feed change and changing temperatures. Most of the isolates showed multiple drug resistance to sulfonamides (Sa), streptomycin (Sm), chloramphenicol (Cp), tetracycline (Tc), kanamycin (Km), spectinomycin (Sp), ampicillin, and/or mercury. Enterotoxigenic E. coli isolates represented four phenotypes: K88+.LT+.Hly+.Raf+.(SaSmCpTcKmSp) (12 strains), K99+.ST+.Raf+. (TcKm) (7 strains), ST+.Raf+.(TcKm) (7 strains), and ST.+(SaSmKm) (25 strains). The drug resistance determinants were transferable concurrently and some of them mobilized the determinants for K88, LT, Hly, and Raf to an E. coli C strain.Enterotoxigenic Escherichia coli (ETEC) is now recognized as a cause of diarrheal disease in animals and man. Since early reports by Smith and Halls (27, 28), many reports have been published on ETEC (14,19,21,22,26,32), enterotoxins (2, 12), pilus antigens (15, 17), and their plasmids (4,13,24,31,34). The recent knowledge of E. coli diarrhea was adequately reviewed by the World Health Organization (36). However, the epidemiology of ETEC disease needs to be investigated in different geographical areas. Not many investigations on ETEC disease in animals have been published in Japan. The present study reports the appearance of ETEC in feces of piglets with diarrhea in connection with adverse factors of feed changes and temperature changes during the winter.IPresent address:

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Section: Discussionmentioning

confidence: 99%

Appearance of Enterotoxigenic Escherichia coli in Piglets with Diarrhea in Connection with Feed Changes

Shimizu

Terashima

1982

Microbiology and Immunology

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“…We never observed increased resistance to cadmium associated with any of the Escherchia coli and Pseudomonas aeruginosa plasmids that were tested. Part of the reason for this may be the inherently greater resistance to cadmium shown by E. coli and P. aeruginosa even in the absence of plasmids (9,22).…”

Section: Methodsmentioning

confidence: 99%

“…Cadmium resistance is the most common resistance determinant found on resistance plasmids (R plasmids) of Staphylococcus aureus, occurring at frequencies of 80% or greater in some some clinical collections (10,16,17,(20)(21)(22). Resistances to antibiotics and to other heavy metals occur at somewhat lower frequencies.…”

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confidence: 99%

Cation Transport Alteration Associated with Plasmid-Determined Resistance to Cadmium in Staphylococcus aureus

Weiss

Silver

Kinscherf

1978

Antimicrob Agents Chemother

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Plasmid-determined resistance to cadmium has only been found with plasmids from Staphylococcus aureus . Resistance to cadmium was associated with a lower accumulation of Cd 2+ ions by the plasmid-bearing resistant cells. Cadmium accumulation by susceptible cells was energy dependent and had those characteristics usually associated with a transmembrane active transport system. There was a specific interrelationship between cadmium accumulation and manganese accumulation and retention. Cd 2+ inhibited the uptake of Mn 2+ and accelerated the loss of intracellular Mn 2+ by the susceptible cells, but was without effect on Mn 2+ transport in resistant S. aureus cells. Under similar conditions, there was no differential effect of Cd 2+ on Mg 2+ , Zn 2+ , Co 2+ , Ni 2+ , or Rb + accumulation or exchange between the susceptible and the resistant strains.

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“…Furthermore, a high degree of DNA-sequence homology has been found between the virulence-associated plasmids of S. dublin and S. typhimurium (Baird, Manning & Jones, 1985) and between these plasmids and those of other salmonella serotypes (Popoff et al 1984). In Resistance to antibacterial agents and heavy metals Bacterial susceptibility to a number of antimicrobial agents and heavy metals was ascertained as previously described (Bauer et al 1966;Ericsson & Sherris, 1971;Anderson & Threlfall, 1974;Nakahara et al 1977). The following antibiotics were tested: ampicillin (Ap), 10 jug; carbenicillin (Cb), 100,ug; cephalothin (CD,30 ,ug;chloramphenicol (Cm) …”

Section: Introductionmentioning

confidence: 99%

Molecular relationship amongSalmonella dublinisolates identified at the Center for Enterobacteriaceae of Palermo during the years 1971–85

et al. 1987

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SUMMARYA molecular epidemiological study was carried out on 60Salmonella dublinisolates identified at the Southern Italy Enterobacteriaceae Center between 1971 and 1985. These included 23 isolates from children with diarrhoea in Palermo obtained during 1984.All isolates from the outbreak of gastroenteritis in children were resistant to chloramphenicol and streptomycin and harboured two plasmids of 50 MDa and 3 MDa molecular weight, whereas the majority of the isolates identified before 1984 were susceptible to these antibiotics and carried only a 50 MDa molecular weight plasmid. FourS. dublinstrains successively identified from cattle (Palermo, Foggia, Portici) and from a child (Palermo) were shown to possess similar antibiotic resistance patterns and plasmid profiles toS. dublinisolates from the outbreak of gastroenteritis in children.The 50 MDa plasmid was shown to be associated with virulence in mice, while it was not possible to assign any genetic function to the 3 MDa plasmid.

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Frequency of heavy-metal resistance in bacteria from inpatients in Japan

Cited by 102 publications

References 20 publications

Appearance of Enterotoxigenic Escherichia coli in Piglets with Diarrhea in Connection with Feed Changes

Appearance of Enterotoxigenic Escherichia coli in Piglets with Diarrhea in Connection with Feed Changes

Cation Transport Alteration Associated with Plasmid-Determined Resistance to Cadmium in Staphylococcus aureus

Molecular relationship amongSalmonella dublinisolates identified at the Center for Enterobacteriaceae of Palermo during the years 1971–85

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