Abstract:In order to clarify the failure to isolate Shigella spp. by direct plating, we compared frequencies of Shigella spp. isolation by direct plating and by plating after enrichment in selenite broth. A total 67 strains were isolated in this study. The strains of 38 (56.7%) were isolated only by direct plating, and 25 (37.3%) strains were isolated by both direct plating and after enrichment. Four strains (6.0%) were isolated after enrichment but not by direct plating. Since 6% of isolated Shigella spp. were not iso… Show more
“…53 After incubation, surviving colonies are streaked onto triple sugar iron agar to isolate Shigella species. Use of transport media (e.g., Cary -Blair, buffered glycerol saline) improves the yield of stool samples for Shigella .…”
“…53 After incubation, surviving colonies are streaked onto triple sugar iron agar to isolate Shigella species. Use of transport media (e.g., Cary -Blair, buffered glycerol saline) improves the yield of stool samples for Shigella .…”
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