Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

Jacinto, Rogério de Castilho; Montagner, Francisco; Signoretti, Fernanda Graziela Corrêa; Almeida, Geovania C.; Gomes, Brenda Paula Figueiredo de Almeida

doi:10.1016/j.joen.2008.08.036

Cited by 63 publications

(45 citation statements)

References 33 publications

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“…Clinical procedures and sample collection procedures were adapted from Jacinto et al 7 After application of local anesthesia, antisepsis of the child's oral cavity was performed with 0.12% chlorhexidine gluconate. The involved tooth received coronary polishing and was isolated with a rubber dam.…”

Section: Clinical Proceduresmentioning

confidence: 99%

An investigation of the presence of specific anaerobic species in necrotic primary teeth

et al. 2013

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Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC and RC samples were collected from 15 subjects and analyzed by PCR for the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii. The frequency of each species was determined in the PC and RC of each case. The species most frequently detected in PCs were P. nigrescens (86.7%), P. gingivalis (73.3%), and F. alocis (73.3%). Of the PC samples, 13.3% contained P. micra and T. denticola, and 6.7% contained T. forsythia. The species most frequently detected in RCs were P. gingivalis (100%) and P. nigrescens (93.3%). P. tannerae, P. micra, and T. denticola were found in 40% of the RC samples; T. forsythia was found in 26.7% of the RC samples. The "red complex", which comprises P. gingivalis, T. denticola, and T. forsythia, was not found in the PC of any tooth but was found in 30% of the RC samples. The detection of P. nigrescens in the PC was statistically associated with the presence of P. nigrescens in the RC (p = 0.04). The results suggest high heterogeneity among the samples, even among those from the same subject.

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Section: Clinical Proceduresmentioning

confidence: 99%

An investigation of the presence of specific anaerobic species in necrotic primary teeth

et al. 2013

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“…N. perflava and N. mucosa have also been associated with meningitis (Saez Nieto et al, 1998). F. nucleatum and P. nigrescens are normal flora of the mouth, which can cause significant periodontal disease (Falagas and Siakavellas, 2000;Jacinto et al, 2008). Workers have suggested that F. nucleatum forms synergistic relationships with Prevotella spp.…”

Section: Potentially Pathogenic Bacteria Were Able To Colonize Et Tubmentioning

confidence: 99%

Endotracheal tube biofilm inoculation of oral flora and subsequent colonization of opportunistic pathogens

Perkins

Woeltje

Angenent

2010

International Journal of Medical Microbiology

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“…O E-test é um teste padrão amplamente utilizado em microbiologia médica, fácil de utilizar e interpretar, além de ser confiável para 206 se testar a suscetibilidade antimicrobiana de bactérias envolvidas em infecções endodônticas 16,24 . De acordo com os resultados obtidos, levando-se em consideração os valores de concentração inibitória mínima (CIM) determinados pela NCCLS, todas as cepas de E. faecalis, E. faecium, A. viscosus e S. aureus mostraram-se 100% suscetíveis a Benzilpenicilina (PG), Amoxicilina (AC), Amoxicilina + ácido clavulânico (XL), Vancomicina (VA) e Moxifloxacina (MX), corroborando com os achados de outros estudos 9,17,19,25 .…”

Section: Discussionunclassified

“…De acordo com os resultados obtidos, levando-se em consideração os valores de concentração inibitória mínima (CIM) determinados pela NCCLS, todas as cepas de E. faecalis, E. faecium, A. viscosus e S. aureus mostraram-se 100% suscetíveis a Benzilpenicilina (PG), Amoxicilina (AC), Amoxicilina + ácido clavulânico (XL), Vancomicina (VA) e Moxifloxacina (MX), corroborando com os achados de outros estudos 9,17,19,25 . Este resultado confirma a utilização da penicilina e seus derivados como antibióticos de primeira escolha no tratamento de infecções de origem endodôntica, e a utilização na terapêutica profilática contra a endocardite bacteriana 16,24 .…”

Section: Discussionunclassified

Avaliação da suscetibilidade antimicrobiana de bactérias anaeróbias facultativas isoladas de canais radiculares de dentes com insucesso endodôntico frente aos antibióticos de uso sistêmico

Santi

Barbosa-Ribeiro

Endo

et al. 2015

Rev. odontol. UNESP

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ResumoIntrodução: Bactérias associadas ao insucesso do tratamento endodôntico são capazes de adquirir e expressar resistência aos agentes antimicrobianos comumente empregados para tratar infecções, o que torna necessário, em determinadas situações, a realização de testes laboratoriais para detectar a resistência ou a suscetibilidade antimicrobiana desses micro-organismos. Objetivo: avaliar a suscetibilidade antimicrobiana das cepas de Enterococcus faecalis, Enterococcus faecium, Actinomyces viscosus e Staphylococcus aureus isoladas de canais radiculares de dentes com insucesso endodôntico. Material e método: Cepas clínicas de Enterococcus faecalis (n=3), Enterococcus faecium (n=3), Actinomyces viscosus (n=3) e Staphylococcus aureus (n=3), coletadas in vivo de canais radiculares com insucesso endodôntico, foram testadas quanto à suscetibilidade antimicrobiana por meio do método E-test em duplicata, utilizando os antibióticos: Amoxicilina (AC), Rifampicina (RI), Moxifloxacina (MX), Vancomicina (VA), Tetraciclina (TC), Ciprofloxacina (CI), Cloranfenicol (CL), Benzilpenicilina (PG), Amoxicilina + ácido clavulânico (XL), Doxiciclina (DC), Eritromicina (EM) e Azitromicina (AZ). Resultado: Todas as cepas clínicas testadas foram suscetíveis aos antibióticos AC, XL, PG, DC, MX, TC e VA. Todos os isolados das espécies de S. aureus foram suscetíveis aos 12 antibióticos testados. As cepas de E. faecalis, E. faecium e A. viscosus mostraram padrão de suscetibilidade intermediário contra EM. Algumas cepas de E. faecalis e E. faecium foram resistentes a AZ e RI. Conclusão: As cepas clínicas isoladas dos canais radiculares de dentes com insucesso endodôntico mostraram perfis diferentes de suscetibilidade antimicrobiana e nenhum isolado de E. faecalis e E. faecium apresentou-se suscetível a AZ e EM.Descritores: Endodontia; bactéria; antibióticos. AbstractIntroduction: Bacteria associated with failure endodontic treatment are capable of acquiring and expressing resistance against antimicrobial agents usually used to treat infections, which makes necessary, in some cases, laboratory tests in order to detect the resistance or antimicrobial susceptibility of these microorganisms. Objective: to evaluate the antimicrobial susceptibility of Enterococcus faecalis, Enterococcus faecium, Actinomyces viscosus and Staphylococcus aureus strains isolated from root canals of teeth with failure endodontic treatment. Material and method: Enterococcus faecalis (n=3), Enterococcus faecium (n=3), Actinomyces viscosus (n=3) and Staphylococcus aureus (n=3) clinical strains collected in vivo from root-filled canals with failure endodontic treatment thad been their antimicrobial susceptibility tested by the E-test method in duplicate using the antibiotics: Amoxicillin (AC), Rifampicin (RI), Moxifloxacin (MX), Vancomycin (VA), Tetracycline (TC), Ciprofloxacin (CI), Chloranphenicol (CL), Benzilpenicillin (PG), Amoxicillin + clavulanic acid (XL), Doxycycline (DC), Erythromycin (EM) e Azithromycin (AZ). Result: all the clinical strains tested were susceptib...

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Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

Cited by 63 publications

References 33 publications

An investigation of the presence of specific anaerobic species in necrotic primary teeth

An investigation of the presence of specific anaerobic species in necrotic primary teeth

Endotracheal tube biofilm inoculation of oral flora and subsequent colonization of opportunistic pathogens

Avaliação da suscetibilidade antimicrobiana de bactérias anaeróbias facultativas isoladas de canais radiculares de dentes com insucesso endodôntico frente aos antibióticos de uso sistêmico

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