Frequency and Type-distribution of Human Papillomavirus from Paraffin-embedded Blocks of High Grade Cervical Intraepithelial Neoplasia Lesions in Thailand

Abstract

Cervical cancer is the most important female gynecological cancer, the second leading cause of cancer mortality in women worldwide and the second most common cancer in Thai women. The major cause of cervical cancer is persistent infection of human papillomavirus (HPV), leading to abnormal epithelial lesions, with progression to precancerous and invasive cancer. This study was conducted to investigate the frequency and type distribution of HPV in Thai women who had abnormal cytology. HPV detection from FFPE confirmed abnormal of high grade cervical intraepithelial lesions were for SPF-10-Innogenic Line Probe Assay. HPV-positivity was detected in 320/355 cases (90.14%) and HPV-negativity in 35/355 (9.86%). HPV-positive was found 147/320 cases (41.4%) of single infection, whereas 173/320 cases (48.7%) showed the multiple HPV infection. The most common seven types were HPV-16, -52, -18, -11, -51, -31 and -33, in that order. HPV 16 and 18, the important oncogenic HPV type, were observed in 64.8% of HSIL cases. Interestingly, a high proportion of multiple infections was found in this study and more than ten types could be detected in one case. Therefore, HPV infection screening program in women is essential, particularly in Thailand. Effective primary and secondary prevention campaigns that reinforce HPV screening for HPV detection and typing may be decrease the incidence and mortality of cervical cancer in the future and may lead to significantly improve the quality of life in Thai women.

HPV Detection and Genotyping in Vulvar Squamous Cell Carcinoma in Northern Thailand

“…The role of HPV as an etiologic factor of SCC in the other sites of anogenital tract has been increasingly recognized and genotype distribution has been assessed in cervical lesions in Thailand (Natphopsuk et al, 2013;Swangvaree et al, 2013). In vulvar carcinoma, the overall prevalence of HPV infection has been reported as 40.4%, which is lower than that of anal cancer in women (90.8%) and vaginal cancer (69.9%) (De Vuyst et al, 2009).…”

Human Papillomavirus Genotypes Profile in Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital, Bandung, Indonesia

“…The discrepancy in this result might be due to the different tests used in various study sites which may have different specificity for HPV detection (Panigoro et al, 2013). For example, in the neighboring countries of Indonesia several tests were used i.e LightPower iVAHPV Genotype RDB Kit in Vietnam (Vu et al, 2012), INNO-LiPA HPV genotyping Extra Amp in Thailand (Swangvaree, et al, 2013), the biotin labeled PCR genotyped using reverse line blot hybridization (RLBH) in North Thailand (Natphopsuk et al, 2013), and many other tests available in the market. The biospecimens used in HPV genotypes detection were also various ranging from cervical swab, parafine block, fresh or frozen biopsy like in our study.…”

Molecular Genotyping of Human Papillomavirus L1 Gene in Low-Risk and High-Risk Populations in Bangkok

“…The 6 genotypes detected in this study have been reported previously in Thai women and of particular interest are the oncogenic types HPV 16 and 18. (18, 34, 35) A prospective case-control study in Northeast Thailand concluded that 66% of cervical cancer cases was caused by HPV 16 or 18, which increased the risk of cervical cancer with an odds ratio of 130. (36) Others have reported that the majority of HPV positive female sex workers in Thailand harbored the oncogenic genotypes HPV 16 or 18.…”