Frequency and diversity of small plasmids in mesophilic Aeromonas isolates from fish, water and sediment

Pérez-García, Daniel; Larios-Serrato, Violeta; Rojas-Rios, Rogelio; Otero-Olarra, Jorge Erick; Mendoza‐Sanchez, Itza; Curiel-Quesada, Everardo; Pérez-Valdespino, Abigail

doi:10.1016/j.plasmid.2021.102607

Cited by 7 publications

(8 citation statements)

References 83 publications

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“…However, several studies have identified small cryptic plasmids in bacterial isolates that contain no recognizable replication machinery [89][90][91][92] . It has been speculated that cryptic plasmids may play roles in viral defense 93 or functionally serving as empty backbones acquisition of novel genes 94 Additionally, some of these cryptic elements may represent currently undescribed classes of MGEs. In recent years, numerous new classes of MGEs have been identified and described in the literature 95,96 .…”

Section: Discussionmentioning

confidence: 99%

Mixed Waste Contamination Selects for a Mobile Genetic Element Population Enriched in Multiple Heavy Metal Resistance Genes

Goff,

Lui,

Nielsen

et al. 2023

Preprint

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Mobile genetic elements (MGEs) like plasmids, viruses, and transposable elements can provide fitness benefits to their hosts for survival in the presence of environmental stressors. Heavy metal resistance genes (HMRGs) are frequently observed on MGEs, suggesting that MGEs may be an important driver of adaptive evolution in environments contaminated with heavy metals. Here, we report the meta-mobilome of the heavy metal contaminated regions of the Oak Ridge Reservation (ORR) subsurface. This meta-mobilome was compared to one derived from samples collected from unimpacted regions of the ORR subsurface. We assembled 1,615 unique circularized DNA elements that we propose to be MGEs. The circular elements from the highly contaminated subsurface were enriched in HMRG clusters relative to those from the nearby unimpacted regions. Additionally, we found that these HMRGs were associated with Gamma and Betaproteobacteria hosts in the contaminated subsurface and potentially facilitate the persistence and dominance of these taxa in this region. Finally, the HMRGs were associated with conjugative elements, suggesting their potential for future lateral transfer. We demonstrate how our understanding of MGE ecology, evolution, and function can be enhanced through the genomic context provided by completed MGE assemblies.

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Section: Discussionmentioning

confidence: 99%

Mixed Waste Contamination Selects for a Mobile Genetic Element Population Enriched in Multiple Heavy Metal Resistance Genes

Goff,

Lui,

Nielsen

et al. 2023

Preprint

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“…This difference in A . hydrophila biochemical profile might be related to their plasmid diversity as plasmids contain genetic determinants responsible for their replication and stability, but they may also carry genes that help bacteria adapt to different environments [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of environmental aquatic bacteria by mass spectrometry supported by biochemical differentiation

et al. 2022

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In this study, the purposefulness of using the API20E biochemical identification system as a supportive tool for enhancing the discrimination of environmental bacteria by MALDI-TOF MS method was evaluated. The identification results of MALDI-TOF MS and API20E for 321 Gram-negative strains isolated from the riverine freshwater and its sediment, and from the tissues of fish from the same water body were compared. Of 190 isolates identified with probable to highly probable species-level identification, and secure genus to probable species identification, 14 isolates (7.37%) had identification score over 2.300, and from the same group 19 isolates (10%) had excellent or very good identification to the genus by API20E system. With regard to agreement at genus level, out of 231 strains with genus designation available by API20E at any level of identification reliability, MALDI-TOF MS genus identification agreed in 163 (70.6%) strains. Of these, 135 (82.8%) were Aeromonas species and the remaining isolates belonged to 7 different genera. Although API20E resulted in frequent misidentification due to a limited profile index, its individual biochemical reactions might contribute to overall characterization of isolates. For example, for all reliable A. hydrophila strain identifications with MALDI-TOF MS, ONPG, GLU and OX reactions were unarguably positive for all fish and water/sediment isolates, whereas only fish isolates yielded additional 100% positive TDA and VP reactions. Thus, after initial identification with MALDI-TOF MS, environmental isolates with lower identification scores should be further analyzed. Before commencing confirmatory testing with nucleic acid-based methods, biochemical API20E tests could be applied as a purposeful and inexpensive identification support in targeting better identification accuracy. In this study, this was particularly evident with A. hydrophila, Chryseobacterium sp. and Pseudomonas sp. This identification strategy could significantly resolve methodological and cost-related shortcomings frequently occurring with large number of environmental isolates.

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“…Aeromonas species are classified into two main groups: (i) psychrophilic, usually non-motile strains which grow well between 22°C and 28°C, and (ii) mesophilic, motile with desirable growth temperature into 35°C–37°C or higher ( Parker and Shaw, 2011 ; Percival and Williams, 2014 ; Liu, 2015 ; Fernández-Bravo and Figueras, 2020 ). The psychrophilic group is represented by the Aeromonas salmonicida , which is described as an important cold-water fish pathogen ( Fernández-Bravo and Figueras, 2020 ; Pérez-García et al, 2021 ). However, this bacterium has already been detected from blood samples of an infected 20-year-old man diagnosed with endocarditis ( Salehi et al, 2019 ).…”

Section: Pathological Processes and Clinical Manifestationsmentioning

confidence: 99%

“…However, this bacterium has already been detected from blood samples of an infected 20-year-old man diagnosed with endocarditis ( Salehi et al, 2019 ). On the other hand, the mesophilic group is often related to both fish and human infections and is mainly represented by A. hydrophila , A. caviae , and A. veronii , but it embraces the other Aeromonas ’s species excepting the psychrophilic A. salmonicida ( Percival and Williams, 2014 ; Pérez-García et al, 2021 ).…”

Section: Pathological Processes and Clinical Manifestationsmentioning

confidence: 99%

Aeromonas and Human Health Disorders: Clinical Approaches

et al. 2022

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The genus Aeromonas comprises more than 30 Gram-negative bacterial species and naturally inhabitants from aquatic environments. These microorganisms, commonly regarded as pathogens of fish and several other animals, have been gaining prominence on medical trial due to its ability to colonize and infect human beings. Besides water, Aeromonas are widely spreaded on most varied sources like soil, vegetables, and food; Although its opportunistic nature, they are able to cause infections on immunocompromised or immunocompetent patients. Aeromonas species regarded as potential human pathogens are usually A. hydrophila, A. caviae, and A. veronii biovar sobria. The main clinical manifestations are gastrointestinal tract disorders, wound, and soft tissue infections, as well as septicemia. Regarding to antibiotic responses, the bacteria present a diversified susceptibility profile and show inherence resistance to ampicillin. Aeromonas, as an ascending genus in microbiology, has been carefully studied aiming comprehension and development of methods for detection and medical intervention of infectious processes, not fully elucidated in medicine. This review focuses on current clinical knowledge related to human health disorders caused by Aeromonas to contribute on development of efficient approaches able to recognize and impair the pathological processes.

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Frequency and diversity of small plasmids in mesophilic Aeromonas isolates from fish, water and sediment

Cited by 7 publications

References 83 publications

Mixed Waste Contamination Selects for a Mobile Genetic Element Population Enriched in Multiple Heavy Metal Resistance Genes

Mixed Waste Contamination Selects for a Mobile Genetic Element Population Enriched in Multiple Heavy Metal Resistance Genes

Identification of environmental aquatic bacteria by mass spectrometry supported by biochemical differentiation

Aeromonas and Human Health Disorders: Clinical Approaches

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