Frequency and Distribution of Rickettsiae, Borreliae, and Ehrlichiae Detected in Human-Parasitizing Ticks, Texas, USA

Mitchell, Elizabeth A.; Williamson, Phillip; Billingsley, Peggy M.; Seals, Janel P.; Ferguson, Erin E.; Allen, Michael S.

doi:10.3201/eid2202/150469

Cited by 12 publications

(2 citation statements)

References 11 publications

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“…To better characterize the samples identified to contain Rickettsia sequences by microbiome analysis, we performed PCR using Rr.190 70P and Rr.190 602N primers for the Rickettsia ‐specific ompA gene on a subset of Rickettsia ‐positive samples. The resultant amplicons were Sanger sequencing as previously described (Mitchell et al., ). Obtained ompA sequences were compared with the NCBI GenBank nucleotide reference database using BLAST.…”

Section: Methodsmentioning

confidence: 99%

Effects of temperature on bacterial microbiome composition in Ixodes scapularis ticks

2018

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Ixodes scapularis , the blacklegged deer tick, is the principal vector of Lyme disease in North America. Environmental factors are known to influence regional and seasonal incidence of Lyme disease and possibly the endemicity of the disease to the northeastern and upper mid‐western regions of the United States. With a goal to understand the impact of environmental temperature on microbial communities within the tick, we investigated the bacterial microbiome of colony‐reared I. scapularis ticks statically incubated at different temperatures (4, 20, 30, and 37°C) at a constant humidity in a controlled laboratory setting by comparison of sequenced amplicons of the bacterial 16S V4 rRNA gene to that of the untreated baseline controls. The microbiomes of colony‐reared I. scapularis males were distinct than that of females, which were entirely dominated by Rickettsia . In silico removal of Rickettsia sequences from female data revealed the underlying bacterial community, which is consistent in complexity with those seen among male ticks. The bacterial community composition of these ticks changes upon incubation at 30°C for a week and 37°C for more than 5 days. Moreover, the male ticks incubated at 30 and 37°C exhibited significantly different bacterial diversity compared to the initial baseline microbiome, and the change in bacterial diversity was dependent upon duration of exposure. Rickettsia ‐free data revealed a significantly different bacterial diversity in female ticks incubated at 37°C compared to that of 4 and 20°C treatments. These results provide experimental evidence that environmental temperature can impact the tick bacterial microbiome in a laboratory setting.

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Section: Methodsmentioning

confidence: 99%

Effects of temperature on bacterial microbiome composition in Ixodes scapularis ticks

2018

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“…Determining the exact causal organism of these cases is difficult as serologic tests for R. rickettsii cannot reliably differentiate between members of the SFGR family (Vaughn et al, ). There is evidence of R. rickettsii and Rickettsia parkeri as well as several other nonpathogenic species of SFGR family in the tick population in Texas (Mitchell et al, ). Without PCR confirmation, we cannot determine the exact rickettsial pathogen.…”

Section: Discussionmentioning

confidence: 99%

Evidence of locally acquired spotted fever group rickettsioses in Southeast Texas, 2008–2016

Erickson

Gunter

Starke

et al. 2018

Zoonoses and Public Health

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We identified six paediatric case-patients from southeast Texas diagnosed with confirmed or probable Spotted Fever Group Rickettsiosis (SFGR) between 2008 and 2016. Only one case had a history of travel to an endemic area. Clinical and laboratory findings strongly suggest locally acquired Rocky Mountain Spotted Fever in one Houston patient. Public health reporting of SFGR is critical for monitoring emergence in nonendemic regions.

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Red imported fire ant (Solenopsis invicta) aggression influences the behavior of three hard tick species

et al. 2019

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Frequency and Distribution of Rickettsiae, Borreliae, and Ehrlichiae Detected in Human-Parasitizing Ticks, Texas, USA

Cited by 12 publications

References 11 publications

Effects of temperature on bacterial microbiome composition in Ixodes scapularis ticks

Effects of temperature on bacterial microbiome composition in Ixodes scapularis ticks

Evidence of locally acquired spotted fever group rickettsioses in Southeast Texas, 2008–2016

Red imported fire ant (Solenopsis invicta) aggression influences the behavior of three hard tick species

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