Frequencies of Micronuclei in Peripheral Lymphocytes in Korean Populations after Chronic Low-dose Radiation Exposure

An, Mi Young; Kim, So Yeon

doi:10.4142/jvs.2002.3.3.213

Cited by 12 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings were in agreement with other studies examining Korean and Romanian workers exposed to occupational IR, where a higher MN frequency was reported after exposure to chronic low-doses of IR. 31 Additionally, we also conducted DNA damage-related gene expression profiling to reveal changes in gene expression levels following low-dose IR exposure. We concluded that the differently expressed genes were related to duration of IR exposure.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Genomic Instability in Medical Workers Exposed to Chronic Low-Dose X-Rays in Northern China

Fang

et al. 2019

Dose-Response

View full text Add to dashboard Cite

The increasing use of ionizing radiation (IR) in medical diagnosis and treatment has caused considerable concern regarding the effects of occupational exposure on human health. Despite this concern, little information is available regarding possible effects and the mechanism behind chronic low-dose irradiation. The present study assessed potential genomic damage in workers occupationally exposed to low-dose X-rays. A variety of analyses were conducted, including assessing the level of DNA damage and chromosomal aberrations (CA) as well as cytokinesis-block micronucleus (CBMN) assay, gene expression profiling, and antioxidant level determination. Here, we report that the level of DNA damage, CA, and CBMN were all significantly increased. Moreover, the gene expression and antioxidant activities were changed in the peripheral blood of men exposed to low-dose X-rays. Collectively, our findings indicated a strong correlation between genomic instability and duration of low-dose IR exposure. Our data also revealed the DNA damage repair and antioxidative mechanisms which could result in the observed genomic instability in health-care workers exposed to chronic low-dose IR.

show abstract

Section: Discussionmentioning

confidence: 99%

Assessment of Genomic Instability in Medical Workers Exposed to Chronic Low-Dose X-Rays in Northern China

Fang

et al. 2019

Dose-Response

View full text Add to dashboard Cite

show abstract

“…The MN assay was used to monitor hospital workers exposed to low doses of ionizing radiation [31] and as a biological dosimeter for radiation therapy patients [32]. The MN index in human populations correlates with age, sex and life-style factors [33]. The reproducibility of individual radiosensitivity with the MN assays is questionable [34].…”

Section: The Micronucleus (Mn) and G2 Assaysmentioning

confidence: 99%

Biomarkers for human radiation exposure

Chaudhry

2008

J Biomed Sci

100

View full text Add to dashboard Cite

There is a concern over the potential use of radioactive isotopes as a weapon of terror. The detonation of a radiation dispersal device, the so-called "dirty bomb" can lead to public panic. In order to estimate risks associated with radiation exposure, it is important to understand the biological effects of radiation exposure. Based on this knowledge, biomarkers to monitor potentially exposed populations after a radiological accident can be developed and would be extremely valuable for emergency response. While the traditional radiation exposure biomarkers based on cytogenetic assays serve as standard, the development of rapid and noninvasive tests for radiation exposure is needed. The genomics based knowledge is providing new avenues for investigation. The examination of gene expression after ionizing radiation exposure could serve as a potential molecular marker for biodosimetry. Microarray based studies are identifying new radiation responsive genes that could potentially be used as biomarkers of human exposure to radiation after an accident.

show abstract

“…Efforts have been made in the development of techniques for measuring radiation damage in biological systems. Among these techniques, MN test has shown as a good biomarker of DNA damage, being widely used in environmental monitoring to detect genotoxic agents[27] and also very useful in monitoring a large number of population[28] because of its simplified technique. MN frequencies in human T lymphocytes represent accumulated genetic changes resulting from the spontaneous or induced chromosome breakage/loss induced by physical and chemical agents.…”

Section: Discussionmentioning

confidence: 99%

Micronucleus Assay-based Evaluation of Radiosensitivity of Lymphocytes among Inhabitants Living in High Background Radiation Area of Mamuju, West Sulawesi, Indonesia

et al. 2018

View full text Add to dashboard Cite

Naturally occurring radiation can be found all around us and account for most of the radiation received by human beings each year. Indonesia has a region with high-dose natural radiation located in the suburb of West Sulawesi province with a dose rate up to 2800 nSv/h; however, its impact was not fully understood. The aim of this study was to evaluate the radiosensitivity of 12 peripheral blood lymphocytes of inhabitant from high background radiation area (HBRA) and 10 from normal background radiation area (NBRA) based on cytokinesis-block micronucleus (CBMN) assay after challenged with 1.5 Gy of gamma ray. The analysis of CBMN was done according to standard procedure as per IAEA guidelines, and frequency of binucleate (mitotic) cells with micronuclei (MN) was scored in around 2000 binucleate lymphocytes cells per culture in microscopic analysis. Mean MN frequency for HBRA was lower than that of NBRA (0.121 vs. 0.189) after irradiation, indicating an adaptive response in HBRA group that resulted in less radiosensitivity; however, there was no statistically significant different ( P > 0.05) between these two groups. The MN number was higher in women compared to men for both HBRA (0.15 vs. 0.09) and NBRA (0.216 vs. 0.147) groups. Besides, there was no statistically significant difference ( P > 0.05) in Nuclear Division Index (NDI), as measured in 500 metaphase cells with published formula, between HBRA and NBRA samples (1.24 vs. 1.21). The lower MN frequency prompts us to conclude that there is an adaptive response in the lymphocytes of inhabitants as an indicator of lower radiosensitivity to the high natural radiation exposure. Further studies using large number of samples are required to obtain more comprehensive conclusion along with the assessment of other types of radiosensitivity-related biomarkers.

show abstract

Frequencies of Micronuclei in Peripheral Lymphocytes in Korean Populations after Chronic Low-dose Radiation Exposure

Cited by 12 publications

References 0 publications

Assessment of Genomic Instability in Medical Workers Exposed to Chronic Low-Dose X-Rays in Northern China

Assessment of Genomic Instability in Medical Workers Exposed to Chronic Low-Dose X-Rays in Northern China

Biomarkers for human radiation exposure

Micronucleus Assay-based Evaluation of Radiosensitivity of Lymphocytes among Inhabitants Living in High Background Radiation Area of Mamuju, West Sulawesi, Indonesia

Contact Info

Product

Resources

About