Frementation of biowaste to H2 by Bacillus licheniformis

Kalia, Vipin Chandra; Jain, Sweta; Kumar, Ashok; Joshi, Akshay

doi:10.1007/bf00360893

Cited by 122 publications

(50 citation statements)

References 15 publications

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“…Bacillus spp. have been shown to be unique in their abilities as they can produce both H 2 and PHB from a wide range of substrates such as glucose and biowastes [4,5,12,13,17,21]. A few of these Bacillus spp.…”

Section: Half Bacterial Biomass With Fresh Gm-2mentioning

confidence: 99%

Production of Polyhydroxyalkanoate Co-polymer by Bacillus thuringiensis

et al. 2012

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Integrative processes for the production of bioenergy and biopolymers are gaining importance in recent years as alternatives to fossil fuels and synthetic plastics. In the present study, Bacillus thuringiensis strain EGU45 has been used to generate hydrogen (H 2 ), polyhydroxybutyrate (PHB) and new co-polymers (NP). Under batch culture conditions with 250 ml synthetic media, B. thuringiensis EGU45 produced up to 0.58 mol H 2 /mol of glucose. Effluent from the H 2 production stage was incubated under shaking conditions leading to the production of PHB up to 95 mg/l along with NP of levulinic acid up to 190 mg/l. A twofold to fourfold enhancement in PHB and up to 1.5 fold increase in NP yields was observed on synthetic medium (mixture of M-9?GM-2 medium in 1:1 ratio) containing at 1-2 % glucose concentration. The novelty of this work lies in developing modified physiological conditions, which induce bacterial culture to produce NP.

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Section: Half Bacterial Biomass With Fresh Gm-2mentioning

confidence: 99%

Production of Polyhydroxyalkanoate Co-polymer by Bacillus thuringiensis

et al. 2012

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“…However, their growth was adversely affected at pH 6.0 and below [15]. As far as the influence of pH on the activities of Bacillus are concerned, B. licheniformis could produce 70 l H 2 /kg VS reduced at pH 6.0 and 74 l H 2 /kg VS reduced at pH 7.0 and 8.0 [8].…”

Section: Introductionmentioning

confidence: 97%

“…a facultative anaerobe has the capacity to adjust its metabolism and respiration depending upon the availability of oxygen (O 2 ) [7]. Bacillus grows well under aerobic conditions and can produce H 2 under anaerobic conditions [8][9][10][11]. It has reduced growth rate under anaerobic conditions and induce genes of metabolic pathways such as arginine deiminase, formate dehydrogenase and pyruvate formate-lyase [7].…”

Section: Introductionmentioning

confidence: 99%

Hydrogen and Polyhydroxybutyrate Producing Abilities of Bacillus spp. From Glucose in Two Stage System

2011

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Metabolic activities of four Bacillus strains to transform glucose into hydrogen (H 2 ) and polyhydroxybutyrate (PHB) in two stages were investigated in this study. Under batch culture conditions, Bacillus thuringiensis EGU45 and Bacillus cereus EGU44 evolved 1.67-1.92 mol H 2 /mol glucose, respectively during the initial 3 days of incubation at 37°C. In the next 2 days, the residual glucose solutions along with B. thuringiensis EGU45 shaken at 200 rpm was found to produce PHB yield of 11.3% of dry cell mass. This is the first report among the non-photosynthetic microbes, where the Bacillus spp.-B. thuringiensis and B. cereus strains have been shown to produce H 2 and PHB in same medium under different conditions.

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“…The hydrolysis of organic matters is influenced by its composition, the most difficult to digest are the lignocellulosic biowaste [5]. Another issue which demands attention is the fact that although H 2 is an intermediate of the AD process, however, in nature, it results in CH 4 as the final byproduct with little or no H 2 evolution [6]. It is because of the fact that thermodynamically, H 2 production process is not stable and the equilibrium shifts to CH 4 production.…”

Section: Introductionmentioning

confidence: 99%

“…Sterilization of biowaste to get rid of contaminating bacteria is a costly proposal. Attempts to produce H 2 from un-sterile wastes have been successful to some extent [6,[15][16][17]. The need is to look for a robust set of organisms, which can survive under harsh conditions and produce H 2 and CH 4 .…”

Section: Introductionmentioning

confidence: 99%

Ecobiotechnological Strategy to Enhance Efficiency of Bioconversion of Wastes into Hydrogen and Methane

et al. 2014

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Vegetable wastes (VW) and food wastes (FW) are generated in large quantities by municipal markets, restaurants and hotels. Waste slurries (250 ml) in 300 ml BOD bottles, containing 3, 5 and 7 % total solids (TS) were hydrolyzed with bacterial mixtures composed of: Bacillus, Acinetobacter, Exiguobacterium, Pseudomonas, Stenotrophomonas and Sphingobacterium species. Each of these bacteria had high activities for the hydrolytic enzymes: amylase, protease and lipase. Hydrolysate of biowaste slurries were subjected to defined mixture of H 2 producers and culture enriched for methanogens. The impact of hydrolysis of VW and FW was observed as 2.6-and 2.8-fold enhancement in H 2 yield, respectively. Direct biomethanation of hydrolysates of VW and FW resulted in 3.0-and 1.15-fold improvement in CH 4 yield, respectively. A positive effect of hydrolysis was also observed with biomethanation of effluent of H 2 production stage, to the extent of 1.2-and 3.5-fold with FW and VW, respectively. The effective H 2 yields were 17 and 85 l/kg TS fed, whereas effective CH 4 yields were 61.7 and 63.3 l/kg TS fed, from VW and FW, respectively. This ecobiotechnological strategy can help to improve the conversion efficiency of biowastes to biofuels.

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Frementation of biowaste to H2 by Bacillus licheniformis

Cited by 122 publications

References 15 publications

Production of Polyhydroxyalkanoate Co-polymer by Bacillus thuringiensis

Production of Polyhydroxyalkanoate Co-polymer by Bacillus thuringiensis

Hydrogen and Polyhydroxybutyrate Producing Abilities of Bacillus spp. From Glucose in Two Stage System

Ecobiotechnological Strategy to Enhance Efficiency of Bioconversion of Wastes into Hydrogen and Methane

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