Freezing of epididymal spermatozoa from dogs after cool storage for 2 or 4 days

Ponglowhapan, Suppawiwat; Chatdarong, Kaywalee; Sirivaidyapong, S.; Lohachit, Chainarong

doi:10.1016/j.theriogenology.2006.01.032

Cited by 29 publications

(45 citation statements)

References 7 publications

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“…In this study, it was observed that after 48 hours of storage of the epididymides at 4˚C, there was no significant difference (p > 0.05) in the mean percent acrosome integrity of the cauda epididymal spermatozoa between different storage groups. This was in agreement with the findings of [4] in beagle dogs and [34] in a mixture of pure and mixed breeds of dogs. Similar findings were obtained in Red Sokoto goats after storage of their epididymides at 4˚C for 48 hours [15] and also in ram [19].…”

Section: Discussionsupporting

confidence: 92%

“…However, [4] and [33] did not detect any significant difference (p > 0.05) in the livability of canine epididymal spermatozoa after 48 hours of storage at 4˚C. Furthermore, a study showed that though there was no significant difference (p > 0.05) in sperm livability after 48 hours (day 2), the sperm livability decreased significantly after 96 hours of storage of cauda epididymal spermatozoa from a variety of pure and mixed breeds of dogs at 5˚C [34]. Taken together, these researchers observed variations in the value of livability of canine epididymal spermatozoa after 48 hours of refrigeration.…”

Section: Discussionmentioning

confidence: 91%

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Effect of Storage Time on Cauda Epididymal Sperm Parameters of Nigerian Local Dogs

Chima¹,

Abu²,

Dawuda³

et al. 2017

OJVM

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Aim: Storage of testes-epididymides at refrigeration temperature is a promising method of short-term sperm preservation for use in reproductive biotechnology in dogs. This study aimed to determine the effect of storage time at 4˚C on the quality of Nigerian local dogs' cauda epididymal spermatozoa recovered post-castration. Materials and Methods: Testes along with their epididymides collected immediately after castration of local dogs were either processed immediately (0th hour) or stored at 4˚C in the refrigerator for 12th, 24th or 48th hours and then processed. Each storage group comprised 5 pairs of testes-epididymides. Sperm motility, concentration, livability, acrosome integrity and morphology from the various storage groups were evaluated and means with standard error of the mean (±SEM) were recorded. Results: Mean percent sperm motility at 0th hour (77.0 ± 2.6) decreased significantly (p < 0.05) after 12 hours (68.0 ± 1.2), 24 hours (66.0 ± 2.4) and 48 hours (60.0 ± 1.6) of storage at 4˚C. No significant difference (p > 0.05) was found when the mean sperm concentrations (×10 7 /ml) of the storage groups (5.86 ± 47, 5.24 ± 26 and 5.38 ± 31) were compared with the control (6.48 ± 0.75). Mean percent sperm livability did not differ significantly (p > 0.05) between the 0th, 12th, 24th and 48th hour groups (86.4 ± 1.5, 78.0 ± 3.1 and 75.0 ± 1.8 respectively). However, there was a significant decrease (p < 0.05) in mean percent sperm livability after 48 hours of storage (69.8 ± 4.2) as compared with the 0th hour group. No significant difference (p > 0.05) was detected in the mean percent acrosome integrity between the 0th, 12th, 24th and 48th hour groups (90.0 ± 1.5, 83.0 ± 3.5, 84.6 ± 4.6 and 82.2 ± 3.9 respectively). A significant increase (p

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 91%

Effect of Storage Time on Cauda Epididymal Sperm Parameters of Nigerian Local Dogs

Chima¹,

Abu²,

Dawuda³

et al. 2017

OJVM

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“…La motilidad total antes de la criopreservación fue superior a la reportada por Tittarelli et al El mayor porcentaje de motilidad total luego de la criopreservación obtenido por Ponglowhapan et al (2006) pudo deberse a que después del descongelamiento, la pajilla fue vaciada en una solución Tris-fructosa citrato a 37 ºC, lo cual pudo permitir la liberación de algunas sustancias esenciales utilizadas en la congelación de semen como el glicerol, y que algunos autores sugieren que ejerce efectos tóxicos en el espermatozoide (Curry, 2000;Holt, 2000).…”

Section: Discussionunclassified

“…Los resultados de integridad funcional de membrana antes y después de la criopreservación son similares a los encontrados por Ponglowhapan et al (2006), donde tampoco se encontraron diferencias significativas; sin embargo, son ligeramente inferiores a los encontrados por Tittarelli et al (2006). En el presente estudio, la membrana plasmática, a pesar de no ser afectada tan drásticamente como la motilidad, también se vio afectada por los procesos de autolisis y congelación.…”

Section: Discussionunclassified

Determinación Del Tiempo Máximo Para Recuperar Y Criopreservar Espermatozoides Obtenidos De La Cola Del Epidídimo en Caninos Post Orquiectomía.

et al. 2011

Rev. investig. vet. Perú

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RESUMENEl objetivo del estudio fue determinar el tiempo máximo para recuperar y criopreservar espermatozoides obtenidos de la cola del epidídimo de caninos post orquiectomía. Se obtuvo testículos de 20 caninos entre 1 y 8 años de edad. Los testículos se colocaron en cloruro de sodio al 0.9% y se almacenaron a 5 ºC durante 0, 24, 48 y 72 horas. Los espermatozoides fueron recuperados al cortar la cola del epidídimo en un dilutor en base a Tris-citrato-fructosa. Para el proceso de criopreservación, se añadió yema de huevo (20%) y glicerol (5%) a la muestra diluida (espermatozoides + dilutor). La nueva dilución fue envasada en pajillas de 0.5 ml y sometidas a una curva de enfriamiento para luego ser colocadas en nitrógeno líquido. Se evaluó motilidad total, motilidad progresiva e integridad funcional de membrana. Previo a la criopreservación, la motilidad total varió significativamente después de 24 y 72 horas de almacenamiento a 5 ºC (p<0.05). Sin embargo, la motilidad total encontrada después del proceso de criopreservación sólo varió después de 72 horas de almacenamiento a 5 ºC (p<0.05). Similar tendencia se observó para la motilidad progresiva. La integridad funcional de membrana plasmática, tanto antes como después del proceso de criopreservación, no sufrió cambios significativos entre los grupos. Los resultados demuestran que es posible recuperar y criopreservar espermatozoides epididimarios hasta después de 48 horas de la orquiectomía del animal.Palabras clave: espermatozoide, epidídimo, canino, criopreservación de semen ABSTRACTThe aim of the study was to determine the maximum time to recover and cryopreserve spermatozoa from the tail of canine epididymis post orchiectomy. The testes were obtained through orchiectomy from 20 dogs aged 1 to 8 years. The testis were placed in sodium chloride 0.9% and stored at 5 °C for 0, 24, 48 and 72 hours. Spermatozoa were recovered by cutting the tail of the epididymis in an extender based on Tris-citrate-fructose. For the

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“…Epididymal spermatozoa have been harvested from a variety of species like cats (Filliers et al, 2008;Hermansson & AxnÈr, 2007), dogs (Garcia-Macias et al, 2006;Hewitt et al, 2001;Nothling et al, 2007;Ponglowhapan et al, 2006), rats (Yamashiro et al, 2007), horses (Braun et al, 1994;Bruemmer, 2006;Heise et al, 2011;Johnson & Coutinho da Silva, 2008;Melo et al, 2008), cattle (Goovaerts et al, 2006;Martins, Rumpf, Pereira, & Dode, 2007), pigs (Ikeda et al, 2002), sheep (Garcia-Macias et al, 2006), goats (Blash, Melican, & Gavin, 2000), red deer (Fernandez-Santos et al, 2006;Garcia-Macias et al, 2006;MartÌnez-Pastor et al, 2006), Spanish Ibex , African buffalo (Herold et al, 2006), North American buffalo (Lessard at al., 2009) and monkeys (Goff et al, 2009;Ng et al, 2002). Pregnancies and offspring after AI with epididymal spermatozoa have been produced amongst others in horses (Barker & Gandier, 1957;Heise et al, 2010;Papa et al, 2008), dogs (Hori, Hagiuda, Kawakami, & Tsutsui, 2005) and Spanish Ibex (SantiagoMoreno et al, 2006).…”

Section: Use Of Epididymal Spermatozoa For Aimentioning

confidence: 99%

Artificial Insemination in Veterinary Science

Heise¹

2012

A Bird's-Eye View of Veterinary Medicine

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Freezing of epididymal spermatozoa from dogs after cool storage for 2 or 4 days

Cited by 29 publications

References 7 publications

Effect of Storage Time on Cauda Epididymal Sperm Parameters of Nigerian Local Dogs

Effect of Storage Time on Cauda Epididymal Sperm Parameters of Nigerian Local Dogs

Determinación Del Tiempo Máximo Para Recuperar Y Criopreservar Espermatozoides Obtenidos De La Cola Del Epidídimo en Caninos Post Orquiectomía.

Artificial Insemination in Veterinary Science

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