Freeze-gelled alginate/gelatin scaffolds for wound healing applications: An in vitro, in vivo study

Afjoul, Homa; Shamloo, Amir; Kamali, Ali

doi:10.1016/j.msec.2020.110957

Cited by 93 publications

(62 citation statements)

References 60 publications

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“…Calcium ions, also known as clotting factor IV, can trigger the intrinsic coagulation cascade along with other clotting factors, thus accelerating the synthesis of enough thrombin to facilitate early fibrin formation [ 56 , 57 ]. Calcium ions mediate the binding of the tenase and prothrombinase complexes to the phospholipid surfaces expressed by platelets to the procoagulant microparticles or microvesicles secreted by them and are required for stable platelet incorporation into the developing thrombus [ 58 ].…”

Section: Calcium and Wound Healingmentioning

confidence: 99%

The Role of Calcium in Wound Healing

Subramaniam

Fauzi

Lokanathan

et al. 2021

IJMS

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Skin injury is quite common, and the wound healing is a complex process involving many types of cells, the extracellular matrix, and soluble mediators. Cell differentiation, migration, and proliferation are essential in restoring the integrity of the injured tissue. Despite the advances in science and technology, we have yet to find the ideal dressing that can support the healing of cutaneous wounds effectively, particularly for difficult-to-heal chronic wounds such as diabetic foot ulcers, bed sores, and venous ulcers. Hence, there is a need to identify and incorporate new ideas and methods to design a more effective dressing that not only can expedite wound healing but also can reduce scarring. Calcium has been identified to influence the wound healing process. This review explores the functions and roles of calcium in skin regeneration and reconstruction during would healing. Furthermore, this review also investigates the possibility of incorporating calcium into scaffolds and examines how it modulates cutaneous wound healing. In summary, the preliminary findings are promising. However, some challenges remain to be addressed before calcium can be used for cutaneous wound healing in clinical settings.

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Section: Calcium and Wound Healingmentioning

confidence: 99%

The Role of Calcium in Wound Healing

Subramaniam

Fauzi

Lokanathan

et al. 2021

IJMS

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“…c. Fluid(exudate) handling properties: The Fluid exudate handling properties of the wafer formulation can be determined by pouring 5 g of SWF in a petri dish placed on the weighing machine and then immersing the wafer formulation in 5 g SWF for 3 hours to know the exudate handling property. At the same time, it will also be used to measured to know the water-absorbing capacity, water holding capacity, and rate of water uptake by the wafer formulation just by pouring a more weighed quantity of SWF until it forms into liquid [3,47].…”

Section: Post Formulation Studies a Sem (Scanning Electron Microscopy)mentioning

confidence: 99%

“…e. DSC studies: Differential Scanning Calorimetry (DSC) is an instrument used to measure enthalpy changes due to change in the material's physical and chemical nature due to exposure to a different temperature [3,4,48]. f. In-vitro studies: In this study, the drug release profile of drug-loaded wafer formulation will be studying using culture plates containing human primary epidermal keratinocytes, Franz diffusion cell, and culture plates containing Gram-Positive and Gram-Negative bacterial growth [46,47,49]. g. In-vivo studies: This study will be performing on the animal by following the guidelines as per the Ethics committee with the permission of IAEC (Institutional Animal Ethical Committee).…”

Section: Foldingmentioning

confidence: 99%

“…Rathnanand, Diabetes should be induced in the rats of either sex with Streptozotocin, and excision of 1×1 cm 2 should be made on the rats after anesthetized with the help of Ketamine HCL + Xylazine. Afterward, wafer formulation is placed with adhesive paper tape, and shrinking of the wound to be measured with a scale and observed for 14 days [29,46,47]. h. Gamma-irradiation: It is a Gamma radiation emitting device that will help sterilize the wafer formulation and know any rheological changes in wafer formulation due to gamma rays [50,51].…”

Section: Mahalaxamimentioning

confidence: 99%

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A Review of Diabetic Foot Ulcer Infections and Lyophilized Wafer Formulation

Jani

Puri

Ubana

2021

JPRI

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Diabetic foot difficulties are the most usually occurring problems globally, resulting in economic disasters for the patients, families, and society. In patients with diabetes, the risk of emerging foot ulcers is 25% high. It has also been in the record that one lower limb amputation occurs every 30 seconds in patients with diabetes worldwide. Novel methods of drug delivery and wound dressing have to develop to solve the lower limb amputation crisis. One such novel method is "Lyophilized wafer formulation." It is an upcoming medicated dressing material that can enhance wound healing and the potential to ingest vast quantities of exudates from Chronic wounds. That can have been formulating by lyophilizing hydrogel of absorbent polymers such as Calcium Alginate, Carrageenan, Thiolated Chitosan, and plasticizer to enhance flexibility withstand the day to day mechanical stress, covered with some adhesive and protective backing layer. Unless it passes evaluation tests such as Fourier-transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC), Scanning electron microscopy (SEM), Exudate handling property, Folding endurance, In-vitro, In-vivo drug release profile, and Gamma-irradiation sterilizes wafer formulation, and it should not administrate directly. Lyophilized wafer formulation will be the most acceptable medicated dressing material in the future that will be useful to treat the normal wound. The wound formation because of diabetic foot ulcer (DFU) infections as there will be site-specific delivery of the drug, packed with an advantage to self administer and easy termination of the drug that can achieve just by removing the wafer case of drug toxicity.

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“…These natural polymer-based scaffolds, however, often hold poor mechanical properties [ 46 , 47 , 48 ]. Hence, a combination of these materials is preferable to provide support for both soft and hard tissues, with a range of mechanical properties and possible facilitated cellular activities [ 49 , 50 , 51 , 52 ].…”

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

et al. 2020

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Tissue engineering (TE) is a highly multidisciplinary field that focuses on novel regenerative treatments and seeks to tackle problems relating to tissue growth both in vitro and in vivo. These issues currently involve the replacement and regeneration of defective tissues, as well as drug testing and other related bioapplications. The key approach in TE is to employ artificial structures (scaffolds) to support tissue development; these constructs should be capable of hosting, protecting and releasing bioactives that guide cellular behaviour. A straightforward approach to integrating bioactives into the scaffolds is discussed utilising polyelectrolyte multilayer capsules (PEMCs). Herein, this review illustrates the recent progress in the use of CaCO3 vaterite-templated PEMCs for the fabrication of functional scaffolds for TE applications, including bone TE as one of the main targets of PEMCs. Approaches for PEMC integration into scaffolds is addressed, taking into account the formulation, advantages, and disadvantages of such PEMCs, together with future perspectives of such architectures.

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Freeze-gelled alginate/gelatin scaffolds for wound healing applications: An in vitro, in vivo study

Cited by 93 publications

References 60 publications

The Role of Calcium in Wound Healing

The Role of Calcium in Wound Healing

A Review of Diabetic Foot Ulcer Infections and Lyophilized Wafer Formulation

Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

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