Freeze-Etched Connective Tissue

Reed, R.

doi:10.1016/b978-0-12-363706-2.50012-x

Cited by 19 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The initial layer built from protein–surface interactions is rigid, whereas the following continuous protein–protein assembly results in a viscous phase, entrapping more water. The high water content of the assembled silk coatings (77%) is near the range of that in connective tissue, which is considered to be 50–75%. , In the silk coatings, the transition from a rigid protein layer to a viscous, water-rich phase could potentially serve as a gradient between a rigid metal implant and the viscoelastic connective tissue that will surround it. Such gradient junctions between implant and tissue have been suggested to lower the risk of implant failure in the context of bridging differences in mechanical properties …”

Section: Discussionmentioning

confidence: 99%

Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study

et al. 2017

View full text Add to dashboard Cite

Functionalization of biomaterials with biologically active peptides can improve their performance after implantation. By genetic fusion to self-assembling proteins, the functional peptides can easily be presented on different physical formats. Herein, a chemical-free coating method based on self-assembly of the recombinant spider silk protein 4RepCT is described and used to prepare functional coatings on various biomaterial surfaces. The silk assembly was studied in real-time, revealing the occurrence of continuous assembly of silk proteins onto surfaces and the formation of nanofibrillar structures. The adsorbed amounts and viscoelastic properties were evaluated, and the coatings were shown to be stable against wash with hydrogen chloride, sodium hydroxide, and ethanol. Titanium, stainless steel, and hydroxyapatite were coated with silk fused to an antimicrobial peptide or a motif from fibronectin. Human primary cells cultured on the functional silk coatings show good cell viability and proliferation, implying the potential to improve implant performance and acceptance by the body.

show abstract

Section: Discussionmentioning

confidence: 99%

Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study

et al. 2017

View full text Add to dashboard Cite

show abstract

“…A second point of interest is that collagen fibrils appear to be formed of a bundle of distinct parallel microfibrils. The existence of microfibrils is still debated in spite of numerous works on vertebrate collagens [8,19,32,36]. In this work, we contribute new evidence on microfibril presence, and the study of their spatial arrangement inside fibrils provides information on the relative orientations of invertebrate collagen molecules.…”

Section: Introductionmentioning

confidence: 92%

“…Replicas were obtained by platinum shadowing at an angle of 45°C and carbon coating. They were rinsed in a sodium hypochlorite solution and washed thoroughly in distilled water [32,38].…”

Section: Optical Microscopymentioning

confidence: 99%

Spatial organization of collagen in annelid cuticle: order and defects

Lepescheux

1988

Biology of the Cell

View full text Add to dashboard Cite

The epidermis of Paralvinella grasslei (Polychaete, Annelida) is covered by an extracellular matrix, the cuticle, mainly composed as in other annelids of superimposed layers of non-striated collagen fibrils. The collagen fibrils of annelid cuticle are shown to be composed of parallel and sinuous microfibrils (thin sections and freeze-fracture replicas). The 3-dimensional organization of collagen is characterized by 2 different types of geometrical order: (a) Fibrils form a quasiorthogonal network, whose structure is comparable to that of a "plywood"; (b) Fibrils are helical, and goniometric studies show that microfibrils present a definite order within each fibril, which is termed "cylindrical twist". These 2 characteristics are those which have recently been evidenced in "blue phases", i.e., liquid crystals which are closely related to cholesteric liquid crystalline phases. Non-fluid analogues of cholesteric liquids are widespread among invertebrate cuticles and the presence of blue phase analogues suggests that a self-assembly mechanisms is involved in cuticle morpho-genesis, which is derived from that governing blue phase growth. The cuticular network presents local rearrangements of fibrils called "defects", despite the fact that they are elaborate structures which trigonal and pentagonal singularities. Branched fibrils are regularly observed. We discuss the involvement of these pattern disruptions in the cuticle growth process.

show abstract

“…Few SEM studies have been carried out to get a three-dimensional picture of connective tissue in health and disease [Forrester et ah, 1969[Forrester et ah, , 1970Hunter and Finlay, 1973;Reed, 1973;Overton and Collins, 1976]. Only stages of collagenous fibre maturation were studied [Forrester et ah, 1970;Rizk, 1979a].…”

Section: Introductionmentioning

confidence: 99%

Stereoscopic visualization of various morphological types of collagenous fibres

Rizk¹

1980

Cells Tissues Organs

View full text Add to dashboard Cite

The connective tissue fibres of various parts of different fascial and aponeurotic planes of the ventral abdominal walls of 26 albino rats were examined with the scanning electron microscope. Five types of collagen fibres could be identified and visualized stereo-scopically: (1) the fine network seen around the muscle fibres; (2) the loose collagenous bundles and their investing reticular network in the deep fasciae; (3) the wavy parallel bundles in the free part of the aponeuroses; (4) the dense mature collagenous bundles and matted collagenous masses in the attached part of the aponeursoes, and (5) the immature fine irregular fibrils seen during healing of wounds. The relationship between these various types and the process of fibrillogenesis of collagenous bundles is discussed. The functional significance of each type is also pointed out.

show abstract

Freeze-Etched Connective Tissue

Cited by 19 publications

References 24 publications

Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study

Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study

Spatial organization of collagen in annelid cuticle: order and defects

Stereoscopic visualization of various morphological types of collagenous fibres

Contact Info

Product

Resources

About