Freeze-drying of ATP entrapped in cationic, low lipid liposomes

Vincourt, Véronique; Nguyễn, Luân; Chaumeil, Jean‐Claude; Dumortier, G.

doi:10.1016/j.cryobiol.2010.01.004

Cited by 11 publications

(3 citation statements)

References 31 publications

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“…The stress factors can be controlled to a large extent by altering process parameters such as the freezing rate, freezing temperature, processing time, type and amount of lyoprotectant [43] . In a study by Vincourt et al, the influence of lyoprotectant/lipid ratio on the particle size of SPC (soybean phosphatidylcholine)/cholesterol/DOTAP liposomal formulations was evaluated; interestingly, the mean particle size increased, remained constant or reduced after lyophilization when a low (3:1), moderate (5:1) or high (10:1) ratio of sugar/lipid was used, respectively [48] . Table 1 also shows average particle size, PDI and zeta potential of lipoplexes compared to the corresponding liposomes.…”

Section: Particle Size and Surface Charge Of Liposomes And Lipoplexesmentioning

confidence: 99%

Development of a DNA-liposome complex for gene delivery applications

Rasoulianboroujeni

Kupgan

Moghadam

et al. 2017

Materials Science and Engineering: C

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Section: Particle Size and Surface Charge Of Liposomes And Lipoplexesmentioning

confidence: 99%

Development of a DNA-liposome complex for gene delivery applications

Rasoulianboroujeni

Kupgan

Moghadam

et al. 2017

Materials Science and Engineering: C

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“…Vincourt et al examined the effect of cryoprotectant (sucrose)/lipid ratio on the particle size of SPC/Cholesterol/DOTAP liposomal formulations. Interestingly, particle size declined significantly through lyophilization when a high ratio of sucrose/lipid (10:1) was employed [33] . In another interesting study, Wieber et al reported a decrease in the size of DOTAP/Cholesterol multilamellar vesicles after freeze-drying [20] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Lyophilization on the Size and Polydispersity of Unilamellar and Multilamellar Liposomes

Tavallaie¹

2016

JNMS

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With the emergence of drug delivery systems, liposome's attracted many attentions as drug and gene delivery vehicles. In this regard, long-term stability of liposomes becomes important in the clinical use of these particles and their success in the market of therapeutics. Lyophilisation is one of the most common methods for preservation of liposome nanoparticles. This process may change some key properties of particles such as size and polydispersity. Hence, in this article we investigated the effects of lyophilization process on these two parameters. A multilamellar liposomal formulation of DOTAP/DOPE/Cholesterol was fabricated through modified lipid film hydration method while extrusion was employed to obtain unilamellar ones. Lyophilization was performed in the presence of sucrose used as the cryoprotectant. The results show that after lyophilization, the mean particle size of both multilamellar and unilamellar vesicles had no significant change (p > 0.1) in contrast to poly dispersity index (p < 0.05

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“…6A, both sugars offered protection against fusion during the lyophilization process, but trehalose showed a more cryoprotective capability with respect to sucrose at every molar ratio tested. However, exceedingly higher or lower sugars concentrations could diminish the cryoprotective effect, as already suggested by previous studies [33,44]; thus, trehalose at lipid:sugar molar ratio of 1:10 was selected (Fig. 6A, B).…”

Section: Lyophilisation Of Liposomal-bortezomibmentioning

confidence: 96%