Non-viral gene delivery systems have attracted many attentions in the field of gene therapy. Liposome nanoparticles are regarded as one of the most effective non-viral gene delivery vectors. With attention to the fact that one important feature of these carries is their transfection efficiency, in this study we compared the efficiency of unilamellar and multilamellar liposomes in transfecting human embryonic kidney cells by LacZ gene. A liposomal formulation made of DOTAP/DOPE/Cholesterol was fabricated through modified lipid film hydration method. In order to obtain unilamellar lipoplexes extrusion was performed. Lipoplexes were prepared by complexation of liposomes with LacZ gene. Zeta potential, size and polydispersity of lipoplexes and liposomes were measured. In order to determine transfection ability of them, LacZ expression was measured using beta-galactosidase assay. The results show that multilamellar liposomes in spite of their larger sizes have higher transfection compared to unilamellar ones. This is indicative of better performance of multilamellar lipoplexes in gene therapy applications.
With the emergence of drug delivery systems, liposome's attracted many attentions as drug and gene delivery vehicles. In this regard, long-term stability of liposomes becomes important in the clinical use of these particles and their success in the market of therapeutics. Lyophilisation is one of the most common methods for preservation of liposome nanoparticles. This process may change some key properties of particles such as size and polydispersity. Hence, in this article we investigated the effects of lyophilization process on these two parameters. A multilamellar liposomal formulation of DOTAP/DOPE/Cholesterol was fabricated through modified lipid film hydration method while extrusion was employed to obtain unilamellar ones. Lyophilization was performed in the presence of sucrose used as the cryoprotectant. The results show that after lyophilization, the mean particle size of both multilamellar and unilamellar vesicles had no significant change (p > 0.1) in contrast to poly dispersity index (p < 0.05
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