Freezability of Cattle and Buffalo Semen and Association of Fresh and Frozen-Thawed Sperm Quality Parameters

Chaudhary, Piyush; Dhami, A. J.; Chaudhari, D. V.; Parmar, S. C.

doi:10.20546/ijcmas.2017.612.162

Cited by 6 publications

(7 citation statements)

References 4 publications

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“…The mean ±SE values, per cent change in post-thaw quality over fresh sperm and the interrelationships amongst sperm motility and velocity/kinematics parameters of fresh semen as well as post-thawed semen assessed by Biovis CASA for Gir, Surti and Murrah bulls semen are presented in Tables 1 to 5. The mean values of initial and post-thaw sperm motility assessed subjectively under microscope for Similar to that of the present findings, the freezability of cattle and buffalo semen has also been previously reported by other workers (Rhodes et al, 1985;Dhami et al, 1990Dhami et al, , 1998Hoflack et al, 2007;Chaudhary et al, 2017). The CASA results reflected positive association between total motile sperm and subjectively assessed sperm motility, and concurred with the report of Patel et al (2012).…”

Section: Resultssupporting

confidence: 92%

“…The conventional subjective evaluation of bovine semen is relatively inaccurate, imprecise, time consuming, and subjected to individual variations (Mortimer et al, 1998;Christensen et al, 2005;Patel et al, 2012), while computer assisted sperm analysis (CASA) is a quite faster, precise and objective tool to identify differences in sperm parameters, related to motility, velocity and morphology, and it avoids subjective errors (Johnson et al, 1996;Amann and Waberski, 2014;Kumar et al, 2018). Moreover, the correlations of the physical attributes of semen with its fertility are highly variable and relatively poor (Shelke and Dhami, 2001;Chaudhary et al, 2017). Now-a-days, there is increasing interest in evaluating sperm motion characteristics by CASA (Amann and Waberski, 2014;Rodriguez-Montaña and Roa-Guerrero, 2017), which covers different velocities of sperm movement (Mortimer et al, 1998;Hoflack et al, 2007;Pathak et al, 2018), and thus provide quantitative assessment of sperms.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Evaluation of Motility and Kinematics of Fresh Versus Frozen-Thawed Spermatozoa of Cattle and Buffalo Bull by CASA

Pathak

Dhami

Chaudhari

et al. 2019

IJAR

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A study was undertaken on semen of three mature bulls each of Gir, Surti and Murrah breed to evaluate the comparative motion characteristics and kinematics of their fresh and frozen-thawed spermatozoa by Biovis CASA. The ejaculates (n= 24/breed) having >75% initial motility were diluted @ 80 million sperm/ml using TFYG extender and were assessed. Amongst motility traits, the total motile, rapid progressive motile and slow progressive motile spermatozoa percentage decreased significantly by 23.08 - 30.09, 43.57 - 55.18, 9.12 - 22.75 %, respectively, plessthan0.01), while non-progressive motile sperm (4.78 - 21.48%) and immotile sperm (164.38 - 178.38 %) percentage increased significantly ( plessthan0.01) in frozen-thawed semen compared to that of fresh semen. The post-thaw quality of semen of all three breeds was in acceptable range. The mean values of sperm velocity/kinematic parameters observed in frozen-thawed semen of Gir, Surti and Murrah bulls, based on total motile sperms, viz., average path velocity, curvilinear velocity, straight line velocity, linearity, straightness, beat-cross frequency, amplitude of lateral head displacement, dancing frequency and dancing mean decreased significantly by 13.70 - 17.79; 9.76 - 12.95; 13.28 - 21.90; 7.28 - 9.68; 4.36 - 7.79; 15.56 - 25.15; 8.78 - 10.50; 6.16 - 18.67 and 12.98 - 15.96 %, respectively, as compared to that of their fresh semen samples. However, wobbling index remained almost same for both fresh and frozen semen. All motility traits differed but none of kinematics/velocity traits differed significantly between breeds/species. The values of all velocity parameters for progressive motile sperms were higher than total motile sperms in all three breeds. The effect of freezing-thawing on velocity and kinematic attributes was much less compared to absolute sperm motility, and both the fresh and frozen-thawed sperms behaved identically with respect to their velocity and kinematics. The rapid progressive motile sperm in both fresh (r=0.41 to 0.92) and frozen-thawed (r=044 to 0.88) semen had significant correlations with most of their velocity traits, and the later were significantly and positively or negatively inter-related among each other in semen of all three breeds. It was therefore concluded that cryopreservation process significantly reduces the motility and kinematics attributes of bovine spermatozoa and, CASA analysis of fresh semen for motility and velocity traits could predict the post-thawed sperm motility and velocity/ kinematics of spermatozoa.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of Motility and Kinematics of Fresh Versus Frozen-Thawed Spermatozoa of Cattle and Buffalo Bull by CASA

Pathak

Dhami

Chaudhari

et al. 2019

IJAR

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show abstract

“…However, no single test or combinations of tests have been proved to be totally reliable for accurate prediction of semen quality in relation to fertility. The correlations of the physical characters with fertility are highly variable and relatively poor (Shelke and Dhami, 2001;Tiwari et al, 2009;Chaudhary et al, 2017). There are numerous…”

Section: Introductionmentioning

confidence: 99%

Correlations of Fresh Sperm Quality and Kinematic Attributes to Those of Post-Thawed Sperms of Bulls Assessed by CASA

Pathak¹,

Dhami²,

Chaudhari³

2018

Int.J.Curr.Microbiol.App.Sci

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“…Hence recently more attention has been given to evaluating sperm motion characteristics using CASA (Amann and Waberski, 2014). The correlations of the physical characters with fertility are highly variable and relatively poor (Shelke and Dhami, 2001;Chaudhary et al, 2017). The literature on the use of CASA in bovine semen laboratory, and the interrelationships of spermatozoal attributes of fresh and cryopreserved bovine semen…”

Section: Correlations Of Motion Characteristics and Kinematicmentioning

confidence: 99%

Correlations of Motion Characteristics and KinematicAttributes of Fresh and Frozen-thawed Spermatozoaof Gir Bulls

Pathak

Dhami

Chaudhari

2019

IJVSBT

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This investigation was carried out on semen of three healthy mature breeding bulls of Gir breed to evaluate the interrelationships among sperm quality attributes of fresh and frozen-thawed semen assessed by Biovis CASA. The ejaculates (n = 24) having >75% initial motility were diluted @80 million sperm/mL using TFYG extender, filled in French mini straws, and were frozen using a programmable bio freezer after 4 hours of equilibration. The straws were thawed in a water bath at 37°C for 30 sec. The freshly diluted and frozenthawed samples were assessed for routine subjective tests and various motion characteristics/kinematics by Biovis CASA. The Pearson’s correlations for sperm motility and velocity/kinematic parameters of total motile sperm as well as of progressively motile sperm were studied in freshly diluted and frozen-thawed semen. In fresh semen, total motile sperm assessed by CASA had significant (p less than 0.05, 01) correlations with rapid progressive motile sperm (r = 0.46), wobbling index (r = 0.52) and dancing frequency (r = -0.43) in fresh semen. In frozen-thawed semen, it was significantly correlated only with linearity (r = 0.46). The rapid progressive motile sperm in both fresh (r = 0.41 to 0.92) and frozen-thawed (r = 044 to 0.88) semen, however, had significant correlations with most of their velocity traits. Further, the average path velocity (VAP), curvilinear velocity (VCL), straight line velocity (VSL), linearity (LIN), straightness (STR), wobbling (WOB), beat-cross frequency (BCF), amplitude of lateral head displacement (ALH), and dancing mean (DNM) of sperm showed significant positive or negative interrelationships among each other in both fresh (r = 0.41 to 0.91) as well as post-thawed (r = 0.44 to 0.90) semen. Moreover, the correlations of motility and kinematics parameters of total motile sperm in both fresh and frozen-thawed semen were highly significant with velocity/kinematics traits of only progressively motile sperm, and the velocity traits among only motile sperm were highly significantly interrelated in both fresh (r = 0.46 to 0.98) and frozen-thawed (r = 0.43 to 0.93) semen of Girbulls, though the magnitudes of correlations were lower in frozen-thawed semen as compared to fresh semen. Thus, CASA analysis offresh semen for motility and velocity traits could predict the post-thawed sperm motility and velocity/kinematics of bovine semen.

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Freezability of Cattle and Buffalo Semen and Association of Fresh and Frozen-Thawed Sperm Quality Parameters

Cited by 6 publications

References 4 publications

Comparative Evaluation of Motility and Kinematics of Fresh Versus Frozen-Thawed Spermatozoa of Cattle and Buffalo Bull by CASA

Comparative Evaluation of Motility and Kinematics of Fresh Versus Frozen-Thawed Spermatozoa of Cattle and Buffalo Bull by CASA

Correlations of Fresh Sperm Quality and Kinematic Attributes to Those of Post-Thawed Sperms of Bulls Assessed by CASA

Correlations of Motion Characteristics and KinematicAttributes of Fresh and Frozen-thawed Spermatozoaof Gir Bulls

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