Freeing Sweet and Sour Chery Trees from Prunus Necrotic Ringspot Virus by Injections of 2,4‐Dioxohexahydro‐1,3,5,‐triazine (DHT)

Bogusch, L. J.; Verderevskaja, T. D.; Schuster, Gottfried

doi:10.1111/j.1439-0434.1985.tb00841.x

Cited by 9 publications

(2 citation statements)

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“…colchesteri. The effect of DHT on a relative wide spectrum of plant viruses has been reported extensively in many plant species, such as cherry, potato, and strawberry, and it is known that treatment reduced the concentration of viruses (Bittner et al, 1987;Bogusch et al, 1985;Borissenko et al, 1985;Kondakova and Schuster, 1991;Schuster et al, 1979). Xu et al (2000) demonstrated that the application of DHT or Virazole reduces the CMV and LSV infection rate in L. longiflorum, and the eradicable effect of DHT on CMV and LSV is higher than that of Virazole (Xu et al, 2000).…”

Section: Second Meristematic Tip Culturementioning

confidence: 99%

Production of Virus-free Bulblets by Meristematic Tip Culture with Antiviral Chemical in Lilium brownii var. colchesteri

Masuda

Thien

Hải

et al. 2011

J. Japan. Soc. Hort. Sci.

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Lilium brownii var. colchesteri has unique and ornamental floral characteristics in graceful harmony with flower and anther colors, flower shape, elegant fragrance, and flower color change from yellowish cream to white during anthesis. There are, however, few accessions conserved in Japan up to the present, and they often show abnormally shaped flowers and mosaic leaves seemingly due to virus infection. Virus-free bulblets were established by combining meristematic tip culture and chemotherapy. At initial diagnosis with RT-PCR, Cucumber mosaic virus (CMV), Lily mottle virus (LMoV) and Lily symptomless virus (LSV) were detected from leaf tissues of the mother plants. All regenerated bulblets obtained by meristematic tip culture of the mother plants were still infected by at least one of the viruses. The meristematic tip of the bulblets infected with either LSV or LMoV was selected for subsequent culture with 2,4-dioxohexahydro-1,3,5-triazine (DHT), an antiviral chemical. LSV was eliminated successfully in mericlones from bulblets with LSV, whereas LMoV was not from those with LMoV. The virusfree bulblets were transferred to new medium without DHT, and multiplied by in vitro scaling. They were then acclimated in a phytotron glass room at 20°C. The plants were confirmed to be virus-free after 18-months' acclimation. It was concluded that the combination of meristem tip culture and chemotherapy is practical for producing virus-free plants of L. brownii var. colchesteri.

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Section: Second Meristematic Tip Culturementioning

confidence: 99%

Production of Virus-free Bulblets by Meristematic Tip Culture with Antiviral Chemical in Lilium brownii var. colchesteri

Masuda

Thien

Hải

et al. 2011

J. Japan. Soc. Hort. Sci.

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“…BiTTNER und SCHENK Unterschiede in der Effektivitat antiphytoviraler Substanzen bei verschiedenen Kartoffelsorten (CASSELS und LONG 1982, BORISSENKO et al 1985, BOGUSCH et al 1985, BiTTNER et al 1987, 1989 Die Inhibitorwirkung in der gegen PVM-resistenteren Sorte 'Galina' ubertraf die Wirkung in den Sorten 'Arkula' und 'Adretta' mit niedrigerer quantitativer PVM-Resistenz {Tab. 2).…”

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Beziehungen zwischen dem Wirkungsgrad antiphytoviraler Substanzen und dem Niveau der quantitativen Sortenresistenz sowie der Virulenz von Virusisolaten an Virus/Wirt‐Systemen der Kartoffel

Bittner

Schenk

1990

Journal of Phytopathology

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Relationship between the efficiency of antiphytoviral substances, the degree of quantitative resistance of cultivars, and the virulence of virus isolates on virus/host‐systems of potato. In vitro systematically infected with potato virus S, potato virus M or potato virus X stem cuttings of different potato varieties were treated with 2,4‐dioxohexahydro‐1,3,5‐triazine and Ribavirin. The reduction of virus replication by chemotherapy differed between varieties depending on their level of quantitative resistance and the virulence of virus isolates. An increasing resistance level of cultivars and a decreasing virulence of the virus isolates resulted in a relative enhancement of the inhibitory of the antiphytoviral substances.

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Antiphytovirale Aktivität von Rhamnolipid aus Pseudomonas aeruginosa

Haferburg

Hommel²,

Kleber

et al. 1987

Acta Biotechnologica

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A rhamnolipid released by Pseudomonas aeruginosa 196 Aa into the culture medium reduced the number of local lesions induced by tobacco mosaic virus on leaves of the hypersensitive host iiicotiana glutinosa L. by up t o 90%. The content of potato virus X in the systemically infected host Nicotiana tabacum L. 'Samsun' is decreased in inoculated as well as in secondarily infected leaves by up to 50%. In a smaller degree red clover mottle virus is influenced in the systemic host Pisum sativum convar. speciosum (Dierb.) Alef 'Sadja'. I n den letzten Jahrzehnten ist eine Anzahl an Verbindungen aufgef linden iind entwickelt worden, mit denen eine Hemniung der Vermehrung oder Ausbreitung von Pflanzenviren moglich ist. Einige von ihnen werden heute bereits zur Viruseliminierung in %ell-, Meristem-und SproBspitzenkulturen eingesetzt [ 1-41. Antiphytovirale Verbindungen finden sich sowohl unter Synthetika als auch unter Extrakten oder Ausscheidungsprodukten von hoheren Organismen und Vikroorganismen. Letztere geben Ansatzpunkte zur biotechnologischen Produktion bzw. fur neue Einsatzgebiete entsprechender Priipamte, z. B. fur lipophile Fraktionen aus der Hefe Lodderomyces elongisprus IMET H 128 [5]. Da letztere ebenso wie antiphytoviral aktive Lysolecithine [6, 71 und Alkansulfonsiiuren [8] neben einer oder zwei hydrophoben, zumeist langeren Alkanketten hydrophile Bereiche unterschiedlicher Struktur besitzen, lag es nahe, auch andere Substenzen ahnlicher Struktur bezuglich antiphytoviraler Aktivitaten zu iintersuchen. Nachfolgend wird uber antiphytovirale Aktivitiiten eines Rhamnolipids berichtet, das von Psezcdomonas aervginosa in das Kulturmedium ausgeschieden wird. Naterial und MethodenKultiviemng und Wachstumskontrolle v m P . aeruginosa P . aeruginosa 196 Aa (Inst. Franpais du Petrole, Frankreich) wurde in Schuttelkultur in einem Minimalmedium nach [9] mit Glycerol (30 g/l) als Kohlenstoff-und Ammoniumsulfat (1 g/l) als Stickstoff-Quelle bei 30°C kultiviert. Der Verlauf der Kultivierung und die Ausscheidung von Rhamnolipid wurde iiber die Messung der optischen Dichte (600 nm) und der Oberflachenspannung verfolgt. Die Konzentration a n oberfliichenaktivem Rhamnolipid murde durch die kritische Micellbildungskonzentration (CMC) er-

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Freeing Sweet and Sour Chery Trees from Prunus Necrotic Ringspot Virus by Injections of 2,4‐Dioxohexahydro‐1,3,5,‐triazine (DHT)

Cited by 9 publications

References 1 publication

Production of Virus-free Bulblets by Meristematic Tip Culture with Antiviral Chemical in Lilium brownii var. colchesteri

Production of Virus-free Bulblets by Meristematic Tip Culture with Antiviral Chemical in Lilium brownii var. colchesteri

Beziehungen zwischen dem Wirkungsgrad antiphytoviraler Substanzen und dem Niveau der quantitativen Sortenresistenz sowie der Virulenz von Virusisolaten an Virus/Wirt‐Systemen der Kartoffel

Antiphytovirale Aktivität von Rhamnolipid aus Pseudomonas aeruginosa

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